HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Koo, B. C. Articles by Kim, T. Search for Related Content PubMed PubMed Citation Articles by Koo, B. C. Articles by Kim, T.

Production of germline transgenic chickens expressing enhanced green fluorescent protein using a MoMLV-based retrovirus vector

Bon Chul Koo^*,1, Mo Sun Kwon^*,1, Bok Ryul Choi^,1, Jin-Hoi Kim^,2, Seong-Keun Cho, Sea Hwan Sohn, Eun Jung Cho, Hoon Taek Lee, Wonkyung Chang^||, Iksoo Jeon^||, Jin-Ki Park^||, Jae Bok Park^¶ and Teoan Kim^*,3

^* Department of Physiology,

^¶ Department of Pathology, Catholic University of Daegu School of Medicine, Daegu, Korea;

Division of Applied Life Science, GyeongSang National University, Jinju, Korea;

Department of Animal Science and Biotechnology, Jinju National University, Jinju, Korea;

Animal Resources Research Center, Konkuk University, Seoul, Korea; and

^|| National Livestock Research Institute, RDA, Suwon, Korea

³Correspondence: Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Korea. E-mail: takim{at}cu.ac.kr

The Moloney murine leukemia virus (MoMLV) -based retrovirus vector system has been used most often in gene transfer work, but has been known to cause silencing of the imported gene in transgenic animals. In the present study, using a MoMLV-based retrovirus vector, we successfully generated a new transgenic chicken line expressing high levels of enhanced green fluorescent protein (eGFP). The level of eGFP expression was conserved after germline transmission and as much as 100 µg of eGFP could be detected per 1 mg of tissue protein. DNA sequencing showed that the transgene had been integrated at chromosome 26 of the G₁ and G₂ generation transgenic chickens. Owing to the stable integration of the transgene, it is now feasible to produce G₃ generation of homozygous eGFP transgenic chickens that will provide 100% transgenic eggs. These results will help establish a useful transgenic chicken model system for studies of embryonic development and for efficient production of transgenic chickens as bioreactors.—Koo, B. C., Kwon, M. S., Choi, B. R., Kim, J-H., Cho, S-K., Sohn, S. H., Cho, E. J., Lee, H. T., Chang, W., Jeon, I., Park, J-K., Park, J. B., Kim, T. Production of germline transgenic chickens expressing enhanced green fluorescent protein using a MoMLV-based retrovirus vector.

Key Words: Moloney murine leukemia virus

This article has been cited by other articles:

				S. S. Shin, T. M. Kim, S. Y. Kim, T. W. Kim, H. W. Seo, S. K. Lee, S. C. Kwon, G. S. Lee, H. Kim, J. M. Lim, et al. Generation of transgenic quail through germ cell-mediated germline transmission FASEB J, July 1, 2008; 22(7): 2435 - 2444. [Abstract] [Full Text] [PDF]

				X. J. Yin, H. S. Lee, X. F. Yu, E. Choi, B. C. Koo, M. S. Kwon, Y. S. Lee, S. J. Cho, G. Z. Jin, L. H. Kim, et al. Generation of Cloned Transgenic Cats Expressing Red Fluorescence Protein Biol Reprod, March 1, 2008; 78(3): 425 - 431. [Abstract] [Full Text] [PDF]