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* INSERM U433, IFR des Neurosciences de Lyon, Faculté de Médecine Laënnec, Lyon, France;
ENS/CNRS 5161, Laboratoires de Biologie Moléculaire de la Cellule, Lyon, France; and
Laboratoire d’Immunologie, Hôpital Neurologique, Lyon, France
2Correspondence: INSERM U433, Faculté de Médecine Laennec, 07 rue Guillaume Paradin, 69372 Lyon, France. E-mail: nataf{at}lyon.inserm.fr
Recent evidence indicates that microglial cells may not derive from blood circulating mature monocytes as they express features of myeloid progenitors. Here, we observed that a subpopulation of microglial cells expressed CD34 and B220 antigens during brain development. We thus hypothesized that microglia, or a subset of microglial cells, originate from blood circulating CD34+/B220+ myeloid progenitors, which could target the brain under developmental or neuroinflammatory conditions. Using experimental allergic encephalomyelitis (EAE) as a model of chronic neuroinflammation, we found that a discrete population of CD34+/B220+ cells expands in both blood and brain of diseased animals. In EAE mice, intravenous transfer experiments showed that macrophage-colony stimulating factor (M-CSF) -expanded CD34+ myeloid progenitors target the inflamed central nervous system (CNS) while keeping their immature phenotype. Based on these results, we then assessed whether CD34+/B220+ cells display in vitro differentiation potential toward microglia. For this purpose, CD34+/B220+ cells were sorted from M-CSF-stimulated bone marrow (BM) cultures and exposed to a glial cell conditioned medium. Under these experimental conditions, CD34+/B220+ cells were able to differentiate into microglial-like cells showing the morphological and phenotypic features of native microglia. Overall, our data suggest that under developmental or neuroinflammatory conditions, a subpopulation of microglial cells derive from CNS-invading CD34+/B220+ myeloid progenitors.—Davoust, N., Vuaillat, C., Cavillon, G., Domenget, C., Hatterer, E., Bernard, A., Dumontel, C., Jurdic, P., Malcus, C., Confavreux, C., Belin, M. F., Nataf, S. Bone marrow CD34+/B220+ progenitors target the inflamed brain and display in vitro differentiation potential toward microglia.
Key Words: glial cell • bone marrow • stem cells • cell transfer
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