| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The FASEB Journal, Vol 2, 3097-3103, Copyright © 1988 by The Federation of American Societies for Experimental Biology
RESEARCH COMMUNICATIONS |
J Lomako, WM Lomako and WJ Whelan
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101.
In this paper we elucidate part of the mechanism of the early stages of the biosynthesis of glycogen. This macromolecule is constructed by covalent apposition of glucose units to a protein, glycogenin, which remains covalently attached to the mature glycogen molecule. We have now isolated, in a 3500-fold purification, a protein from rabbit muscle that has the same Mr as glycogenin, is immunologically similar, and proves to be a self-glucosylating protein (SGP). When incubated with UDP-[14C]glucose, an average of one molecular proportion of glucose is incorporated into the protein, which we conclude is the same as glycogenin isolated from native glycogen. The native SGP appears to exist as a high-molecular-weight species that contains many identical subunits. Because the glucose that is self-incorporated can be released almost completely from the acceptor by glycogenolytic enzymes, the indication is that it was added to a preformed chain or chains of 1,4- linked alpha-glucose residues. This implies that SGP already carries an existing maltosaccharide chain or chains to which the glucose is added, rather than glucose being added directly to protein. The putative role of SGP in glycogen synthesis is confirmed by the fact that glucosylated SGP acts as a primer for glycogen synthase and branching enzyme to form high-molecular-weight material. SGP itself is completely free from glycogen synthase. The quantity of SGP in muscle is calculated to be about one-half the amount of glycogenin bound in glycogen.
This article has been cited by other articles:
|
|
 |
|
  R. J. Wilson, J. E. Gusba, D. L. Robinson, and T. E. Graham Glycogenin protein and mRNA expression in response to changing glycogen concentration in exercise and recovery Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1815 - E1822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Marchand, M. Tarnopolsky, K. B. Adamo, J. M. Bourgeois, K. Chorneyko, and T. E. Graham Quantitative assessment of human muscle glycogen granules size and number in subcellular locations during recovery from prolonged exercise J. Physiol., April 15, 2007; 580(2): 617 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Katz Glycogenin, proglycogen, and glycogen biogenesis: what's the story? Am J Physiol Endocrinol Metab, April 1, 2006; 290(4): E757 - E758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Nilsson, Y. C. Long, S. Martinsson, S. Glund, P. Garcia-Roves, L. T. Svensson, L. Andersson, J. R. Zierath, and M. Mahlapuu Opposite Transcriptional Regulation in Skeletal Muscle of AMP-activated Protein Kinase {gamma}3 R225Q Transgenic Versus Knock-out Mice J. Biol. Chem., March 17, 2006; 281(11): 7244 - 7252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bazan and J. A. Curtino The size of the C-chain maltosaccharide of glycogen: evidence for the presence of only a single branch Glycobiology, October 1, 2005; 15(10): 14C - 18C. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. D. Hurley, S. Stout, E. Miner, J. Zhou, and P. J. Roach Requirements for Catalysis in Mammalian Glycogenin J. Biol. Chem., June 24, 2005; 280(25): 23892 - 23899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Barbier, C. Oesterhelt, M. D. Larson, R. G. Halgren, C. Wilkerson, R. M. Garavito, C. Benning, and A. P.M. Weber Comparative Genomics of Two Closely Related Unicellular Thermo-Acidophilic Red Algae, Galdieria sulphuraria and Cyanidioschyzon merolae, Reveals the Molecular Basis of the Metabolic Flexibility of Galdieria sulphuraria and Significant Differences in Carbohydrate Metabolism of Both Algae Plant Physiology, February 1, 2005; 137(2): 460 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M.J. Langeveld, M. Vennik, M. Kottenhagen, R. van Wijk, A. Buijk, J. W. Kijne, and S. de Pater Glucosylation Activity and Complex Formation of Two Classes of Reversibly Glycosylated Polypeptides Plant Physiology, May 1, 2002; 129(1): 278 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Hahn, A. Blaschitz, E.T. Korgun, I. Lang, G. Desoye, G. Skofitsch, and G. Dohr From maternal glucose to fetal glycogen: expression of key regulators in the human placenta Mol. Hum. Reprod., December 1, 2001; 7(12): 1173 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. F. Hansen, W. Derave, P. Jensen, and E. A. Richter No limiting role for glycogenin in determining maximal attainable glycogen levels in rat skeletal muscle Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E398 - E404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shearer, I. Marchand, P. Sathasivam, M. A. Tarnopolsky, and T. E. Graham Glycogenin activity in human skeletal muscle is proportional to muscle glycogen concentration Am J Physiol Endocrinol Metab, January 1, 2000; 278(1): E177 - E180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mu and P. J. Roach Characterization of Human Glycogenin-2, a Self-glucosylating Initiator of Liver Glycogen Metabolism J. Biol. Chem., December 25, 1998; 273(52): 34850 - 34856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Adamo and T. E. Graham Comparison of traditional measurements with macroglycogen and proglycogen analysis of muscle glycogen J Appl Physiol, March 1, 1998; 84(3): 908 - 913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mu, A. V. Skurat, and P. J. Roach Glycogenin-2, a Novel Self-glucosylating Protein Involved in Liver Glycogen Biosynthesis J. Biol. Chem., October 31, 1997; 272(44): 27589 - 27597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mu, C. Cheng, and P. J. Roach Initiation of Glycogen Synthesis in Yeast. REQUIREMENT OF MULTIPLE TYROSINE RESIDUES FOR FUNCTION OF THE SELF-GLUCOSYLATING Glg PROTEINS IN VIVO J. Biol. Chem., October 25, 1996; 271(43): 26554 - 26560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Alonso, J. Lomako, W. M. Lomako, and W. J. Whelan Catalytic Activities of Glycogenin Additional to Autocatalytic Self-glucosylation J. Biol. Chem., June 23, 1995; 270(25): 15315 - 15319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kozarov, H. van der Wel, M. Field, M. Gritzali, R. D. Brown Jr., and C. M. West Characterization of FP21, a Cytosolic Glycoprotein from Dictyostelium J. Biol. Chem., February 17, 1995; 270(7): 3022 - 3030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Pederson, C. Cheng, W. A. Wilson, and P. J. Roach Regulation of Glycogen Synthase. IDENTIFICATION OF RESIDUES INVOLVED IN REGULATION BY THE ALLOSTERIC LIGAND GLUCOSE-6-P AND BY PHOSPHORYLATION J. Biol. Chem., September 1, 2000; 275(36): 27753 - 27761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. B. NGIMBOUS, F. BOURGEOIS, C. MAS, M. SIMONNEAU, and J.-M. MOALIC Heart transplantation changes the expression of distinct gene families Physiol Genomics, December 21, 2001; 7(2): 115 - 126. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
