FASEB J. Thermo Fisher Scientific
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bruewer, M.
Right arrow Articles by Nusrat, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bruewer, M.
Right arrow Articles by Nusrat, A.
(The FASEB Journal. 2005;19:923-933.)
© 2005 FASEB

Interferon-{gamma} induces internalization of epithelial tight junction proteins via a macropinocytosis-like process

Matthias Bruewer*,{dagger},1, Markus Utech*,{dagger}, Andrei I. Ivanov*, Ann M. Hopkins*, Charles A. Parkos* and Asma Nusrat*,2

* Epithelial Pathobiology Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA; and
{dagger} Department of General Surgery, University of Münster, Münster, Germany

2Correspondence: Department of Pathology and Laboratory Medicine, Emory University, Whitehead Research Building, Room 105E, 615 Michael St., Atlanta, GA 30322, USA. E-mail: anusrat{at}emory.edu

Increased epithelial permeability is observed in inflammatory states. However, the mechanism by which inflammatory mediators such as IFN-{gamma} increase epithelial permeability is unknown. We recently observed that IFN-{gamma} induces disassembly of tight junctions (TJ); in this study we asked whether such TJ disassembly is mediated by endocytosis of junctional proteins. The role of three major internalization pathways in disruption of TJ in IFN-{gamma}-treated intestinal epithelial cells was analyzed using selective inhibitors and markers of the pathways. No role for the clathrin- and caveolar-mediated endocytosis in the IFN-{gamma}-induced internalization of TJ proteins was observed. However, inhibitors of macropinocytosis blocked internalization of TJ proteins and junctional proteins colocalized with macropinocytosis markers, dextran and phosphatidylinositol-3,4,5-trisphosphate. Internalized TJ proteins were identified in early and recycling endosomes but not in late endosomes/lysosomes. These results for the first time suggest that IFN-{gamma} produces a leaky epithelial barrier by inducing macropinoytosis of TJ proteins.—Bruewer, M., Utechm M., Ivanov, A. I., Hopkins, A. M., Parkos, C. A., Nusrat, A. Interferon-{gamma} induces internalization of epithelial tight junction proteins via a macropinocytosis-like process.


Key Words: cytokines • inflammation • mucosa




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
E. A. Severson, L. Jiang, A. I. Ivanov, K. J. Mandell, A. Nusrat, and C. A. Parkos
Cis-Dimerization Mediates Function of Junctional Adhesion Molecule A
Mol. Biol. Cell, May 1, 2008; 19(5): 1862 - 1872.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. Forster, M. Burek, I. A. Romero, B. Weksler, P.-O. Couraud, and D. Drenckhahn
Differential effects of hydrocortisone and TNF{alpha} on tight junction proteins in an in vitro model of the human blood-brain barrier
J. Physiol., April 1, 2008; 586(7): 1937 - 1949.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Yang, C. Qiu, N. Biswas, J. Jin, S. C. Watkins, R. C. Montelaro, C. B. Coyne, and T. Wang
Correlation of the Tight Junction-like Distribution of Claudin-1 to the Cellular Tropism of Hepatitis C Virus
J. Biol. Chem., March 28, 2008; 283(13): 8643 - 8653.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Yamamura, N. Nishimura, H. Nakatsuji, S. Arase, and T. Sasaki
The Interaction of JRAB/MICAL-L2 with Rab8 and Rab13 Coordinates the Assembly of Tight Junctions and Adherens Junctions
Mol. Biol. Cell, March 1, 2008; 19(3): 971 - 983.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
J. Pannequin, N. Delaunay, C. Darido, T. Maurice, P. Crespy, M. A. Frohman, M. S. Balda, K. Matter, D. Joubert, J.-F. Bourgaux, et al.
Phosphatidylethanol Accumulation Promotes Intestinal Hyperplasia by Inducing ZONAB-Mediated Cell Density Increase in Response to Chronic Ethanol Exposure
Mol. Cancer Res., November 1, 2007; 5(11): 1147 - 1157.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. M. Bryant, M. C. Kerr, L. A. Hammond, S. R. Joseph, K. E. Mostov, R. D. Teasdale, and J. L. Stow
EGF induces macropinocytosis and SNX1-modulated recycling of E-cadherin
J. Cell Sci., May 15, 2007; 120(10): 1818 - 1828.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. T. Blikslager, A. J. Moeser, J. L. Gookin, S. L. Jones, and J. Odle
Restoration of Barrier Function in Injured Intestinal Mucosa
Physiol Rev, April 1, 2007; 87(2): 545 - 564.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. M. Al-Sadi and T. Y. Ma
IL-1beta Causes an Increase in Intestinal Epithelial Tight Junction Permeability
J. Immunol., April 1, 2007; 178(7): 4641 - 4649.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
M. BRUEWER, S. SAMARIN, and A. NUSRAT
Inflammatory bowel disease and the apical junctional complex.
Ann. N.Y. Acad. Sci., August 1, 2006; 1072: 242 - 252.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
H. Fujita, H. Chiba, H. Yokozaki, N. Sakai, K. Sugimoto, T. Wada, T. Kojima, T. Yamashita, and N. Sawada
Differential Expression and Subcellular Localization of Claudin-7, -8, -12, -13, and -15 Along the Mouse Intestine
J. Histochem. Cytochem., August 1, 2006; 54(8): 933 - 944.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. Shen, E. D. Black, E. D. Witkowski, W. I. Lencer, V. Guerriero, E. E. Schneeberger, and J. R. Turner
Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure
J. Cell Sci., May 15, 2006; 119(10): 2095 - 2106.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Terai, N. Nishimura, I. Kanda, N. Yasui, and T. Sasaki
JRAB/MICAL-L2 Is a Junctional Rab13-binding Protein Mediating the Endocytic Recycling of Occludin
Mol. Biol. Cell, May 1, 2006; 17(5): 2465 - 2475.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Shen and J. R. Turner
Role of Epithelial Cells in Initiation and Propagation of Intestinal Inflammation. Eliminating the static: tight junction dynamics exposed
Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G577 - G582.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. Stamatovic, O. B. Dimitrijevic, R. F. Keep, and A. V. Andjelkovic
Protein Kinase C{alpha}-RhoA Cross-talk in CCL2-induced Alterations in Brain Endothelial Permeability
J. Biol. Chem., March 31, 2006; 281(13): 8379 - 8388.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
H. Chiba, T. Kojima, M. Osanai, and N. Sawada
The Significance of Interferon-{gamma}-Triggered Internalization of Tight-Junction Proteins in Inflammatory Bowel Disease
Sci. Signal., January 3, 2006; 2006(316): pe1 - pe1.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Utech, A. I. Ivanov, S. N. Samarin, M. Bruewer, J. R. Turner, R. J. Mrsny, C. A. Parkos, and A. Nusrat
Mechanism of IFN-{gamma}-induced Endocytosis of Tight Junction Proteins: Myosin II-dependent Vacuolarization of the Apical Plasma Membrane
Mol. Biol. Cell, October 1, 2005; 16(10): 5040 - 5052.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by The Federation of American Societies for Experimental Biology.