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,1
* Department of Biological Chemistry and Israel Center for Neuronal Computation, The Hebrew University of Jerusalem, Jerusalem, Israel;
School of Chemistry, The Raymond and Beverly Sackler Faculty of Exact Sciences, Tel-Aviv University, Ramat Aviv, Israel; and
Tel Aviv Sourasky Medical Center, Human Brain Imaging Unit, The Wohl Institute for Advanced Imaging, Tel Aviv, Israel
4Correspondence: Department of Biological Chemistry and Israel Center for Neuronal Computation, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. E-mail: soreq{at}cc.huji.ac.il
Cholinergic imbalances occur after traumatic effects and in the initial stages of neurodegenerative diseases, but their long-lasting effects remained largely unexplained. To address this, we used TgS transgenic mice constitutively overexpressing synaptic acetylcholinesterase (AChE-S) and presenting a complex phenotype of progressive neurodeterioration. T1- and T2-weighted magnetic resonance (MR) brain images appeared similar. However, diffusion-weighted MRI showed decreased baseline water apparent diffusion coefficient in the brains of TgS animals. Furthermore, contrast-enhanced MRI after gadolinium diethylenetriaminepentaacetic acid (Gd-DTPA) injection demonstrated slower recovery of normal signals in the TgS brains than with controls. Perfusion MR imaging and difference T1 maps calculated from pre- postcontrast T1-weighted MR images indicated accumulation of more Gd-DTPA molecules in the TgS brains than in the parent strain, reflecting impaired blood-brain barrier (BBB) functioning in these transgenic mice. To explore the molecular mechanism(s) underlying these global phenotypes, we performed microarray analysis in the stress-controlling prefrontal cortex of TgS vs. strain-matched wild-type animals. Profound overexpression of numerous ion channels, transporters, and adhesion genes was confirmed by real time RT-PCR tests. Immunohistochemical and immunoblot analyses revealed corresponding increases in the level and cellular distributions of the chloride channel CLCN3 and the water channel AQP4, both of which contribute to BBB maintenance. Our study attributes to balanced cholinergic neurotransmission, a central role in the brain’s maintenance of water diffusion and ion transport, and indicates that chronic impairments in this maintenance facilitate neurodeterioration through interference with BBB function.—Meshorer, E., Biton, I. E., Ben-Shaul, Y., Ben-Ari, S., Assaf, Y., Soreq, H., Cohen, Y. Chronic cholinergic imbalances promote brain diffusion and transport abnormalities.
Key Words: cholinergic neurotransmission • acetylcholinesterase • blood-brain barrier • aquaporin 4
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