| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto city, Kyoto, Japan
1Correspondence: Department of Pediatrics, Graduate School of Medicine, Kyoto University, Shogoin Kawahara-cho 54, Sakyo-ku, Kyoto city, Kyoto, Japan 606-8507. E-mail: heike{at}kuhp.kyoto-u.ac.jp
We evaluated the expression of the FLK1, one of the lateral mesoderm early markers where cardiogenesis occurs, to characterize and isolate cardiac stem/progenitor cells from ES cells. Dissociated cells from embryoid bodies (EBs) on day 3, 4, or 5 were collected into two subpopulations with or without FLK1 expression and coculture on OP9 stromal cells was continued to examine whether contracting colonies came out or not. FLK1+ cells from EBs at days 3 and 4 formed spontaneous contracting colonies more efficiently than FLK1– cells on the same days, but not at day 5. Most contracting cardiac colonies derived from FLK1+cells mainly on day 4 were detected on endothelial cells along with hematopoietic cells. Further characterization of cells with these capabilities into three lineages revealed the FLK1+ CD31–VE-cadherin– phenotype. Our findings indicate that FLK1+cells, especially FLK1+ CD31–VE-cadherin– cells, could act as cardiohemangioblasts to form cardiac cells as well as endothelial cells and hematopoietic cells.—Iida, M., Heike, T., Yoshimoto, M., Baba, S., Doi, H., Nakahat, T. Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation.
Key Words: embryoid bodies • ES cells • cardiomyocyte differentiation • blastocysts
This article has been cited by other articles:
|
|
 |
|
  S. Baba, T. Heike, M. Yoshimoto, K. Umeda, H. Doi, T. Iwasa, X. Lin, S. Matsuoka, M. Komeda, and T. Nakahata Flk1+ cardiac stem/progenitor cells derived from embryonic stem cells improve cardiac function in a dilated cardiomyopathy mouse model Cardiovasc Res, October 1, 2007; 76(1): 119 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Baba, T. Heike, K. Umeda, T. Iwasa, S. Kaichi, Y. Hiraumi, H. Doi, M. Yoshimoto, M. Kanatsu-Shinohara, T. Shinohara, et al. Generation of Cardiac and Endothelial Cells from Neonatal Mouse Testis-Derived Multipotent Germline Stem Cells Stem Cells, June 1, 2007; 25(6): 1375 - 1383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tsuchiya, T. Heike, S. Baba, H. Fujino, K. Umeda, Y. Matsuda, M. Nomoto, T. Ichida, Y. Aoyagi, and T. Nakahata Long-Term Culture of Postnatal Mouse Hepatic Stem/Progenitor Cells and Their Relative Developmental Hierarchy Stem Cells, April 1, 2007; 25(4): 895 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Lugus, Y. S. Chung, J. C. Mills, S.-I. Kim, J. A. Grass, M. Kyba, J. M. Doherty, E. H. Bresnick, and K. Choi GATA2 functions at multiple steps in hemangioblast development and differentiation Development, January 15, 2007; 134(2): 393 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen, I. Amende, T. G. Hampton, Y. Yang, Q. Ke, J.-Y. Min, Y.-F. Xiao, and J. P. Morgan Vascular endothelial growth factor promotes cardiomyocyte differentiation of embryonic stem cells Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1653 - H1658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Rippon, J. M. Polak, M. Qin, and A. E. Bishop Derivation of Distal Lung Epithelial Progenitors from Murine Embryonic Stem Cells Using a Novel Three-Step Differentiation Protocol Stem Cells, May 1, 2006; 24(5): 1389 - 1398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. W. Chaudhary, N. X. Barrezueta, M. B. Bauchmann, A. J. Milici, G. Beckius, D. B. Stedman, J. E. Hambor, W. L. Blake, J. D. McNeish, A. Bahinski, et al. Embryonic Stem Cells in Predictive Cardiotoxicity: Laser Capture Microscopy Enables Assay Development Toxicol. Sci., March 1, 2006; 90(1): 149 - 158. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
