| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Molecular Neuropsychiatry Branch, National Institute on Drug Abuse-Intramural Research Program, National Institutes of Health, DHHS, Baltimore, Maryland, USA
1 Correspondence: Molecular Neuropsychiatry Branch, National Institute on Drug Abuse, Intramural Research Program, National Institute of Health, DHHS, 5500 Nathan Shock Dr., Baltimore, MD 21224, USA. E-mail: jcadet{at}intra.nida.nih.gov
Methamphetamine (METH) is an illicit drug that causes neurodegenerative effects in humans. In rodents, METH induces apoptosis of striatal glutamic acid decarboxylase (GAD) -containing neurons. This paper provides evidence that METH-induced cell death occurs consequent to interactions of ER stress and mitochondrial death pathways. Specifically, injections of METH are followed by an almost immediate activation of proteases calpain and caspase-12, events consistent with drug-induced ER stress. Involvement of ER stress was further supported by observations of increases in the expression of GRP78/BiP and CHOP. Participation of the mitochondrial pathway was demonstrated by the transition of AIF, smac/DIABLO, and cytochrome c from mitochondrial into cytoplasmic fractions. These changes occur before the apoptosome-associated pro-caspase-9 cleavage. Effector caspases-3 and -6, but not -7, were cleaved with the initial time of caspase-3 activation occurring before caspase 9 cleavage; this suggests possible earlier cleavage of caspase-3 by caspase-12. These events preceded proteolysis of the caspase substrates DFF-45, lamin A, and PARP in nuclear fractions. These findings indicate that METH causes neuronal apoptosis in part via cross-talks between ER- and mitochondria-generated processes, which cause activation of both caspase-dependent and -independent pathways.—Jayanthi, S., Deng, X., Noailles, P.-A. H., Ladenheim, B., Cadet, J. L. Methamphetamine induces neuronal apoptosis via cross-talks between endoplasmic reticulum and mitochondria-dependent death cascades.
Key Words: apoptosis-inducing factor • CHOP transcription factor • BiP/GRP78 • DNA fragmentation
This article has been cited by other articles:
|
|
 |
|
  T. A. Green, I. N. Alibhai, S. Unterberg, R. L. Neve, S. Ghose, C. A. Tamminga, and E. J. Nestler Induction of Activating Transcription Factors (ATFs) ATF2, ATF3, and ATF4 in the Nucleus Accumbens and Their Regulation of Emotional Behavior J. Neurosci., February 27, 2008; 28(9): 2025 - 2032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-j. Huang, C.-y. Lin, L. Haataja, T. Gurlo, A. E. Butler, R. A. Rizza, and P. C. Butler High Expression Rates of Human Islet Amyloid Polypeptide Induce Endoplasmic Reticulum Stress Mediated {beta}-Cell Apoptosis, a Characteristic of Humans With Type 2 but Not Type 1 Diabetes Diabetes, August 1, 2007; 56(8): 2016 - 2027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tan, N. Dourdin, C. Wu, T. De Veyra, J. S. Elce, and P. A. Greer Ubiquitous Calpains Promote Caspase-12 and JNK Activation during Endoplasmic Reticulum Stress-induced Apoptosis J. Biol. Chem., June 9, 2006; 281(23): 16016 - 16024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Hu, Z. Han, A. D. Couvillon, R. J. Kaufman, and J. H. Exton Autocrine Tumor Necrosis Factor Alpha Links Endoplasmic Reticulum Stress to the Membrane Death Receptor Pathway through IRE1{alpha}-Mediated NF-{kappa}B Activation and Down-Regulation of TRAF2 Expression Mol. Cell. Biol., April 15, 2006; 26(8): 3071 - 3084. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-C. Lee, C.-H. Lee, C.-L. Su, C.-W. Huang, H.-S. Liu, C.-N. Lin, and S.-J. Won Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells Carcinogenesis, October 1, 2005; 26(10): 1716 - 1730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ebensperger, R. Ebensperger, E. A Herrera, R. A Riquelme, E. M Sanhueza, F. Lesage, J. J Marengo, R. I Tejo, A. J Llanos, and R. V Reyes Fetal brain hypometabolism during prolonged hypoxaemia in the llama J. Physiol., September 15, 2005; 567(3): 963 - 975. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Thiriet, X. Deng, M. Solinas, B. Ladenheim, W. Curtis, S. R. Goldberg, R. D. Palmiter, and J. L. Cadet Neuropeptide Y Protects against Methamphetamine-Induced Neuronal Apoptosis in the Mouse Striatum J. Neurosci., June 1, 2005; 25(22): 5273 - 5279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hong, M.-y. Lin, J.-m. Huang, P. Baumeister, S. Hakre, A. L. Roy, and A. S. Lee Transcriptional Regulation of the Grp78 Promoter by Endoplasmic Reticulum Stress: ROLE OF TFII-I AND ITS TYROSINE PHOSPHORYLATION J. Biol. Chem., April 29, 2005; 280(17): 16821 - 16828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Yun, K.-H. Kim, M. W. Calcutt, and F. P. Guengerich Kinetic Analysis of Oxidation of Coumarins by Human Cytochrome P450 2A6 J. Biol. Chem., April 1, 2005; 280(13): 12279 - 12291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jayanthi, X. Deng, B. Ladenheim, M. T. McCoy, A. Cluster, N.-s. Cai, and J. L. Cadet Calcineurin/NFAT-induced up-regulation of the Fas ligand/Fas death pathway is involved in methamphetamine-induced neuronal apoptosis PNAS, January 18, 2005; 102(3): 868 - 873. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
