HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by MORDELET, E. Articles by GRANON, S. Search for Related Content PubMed PubMed Citation Articles by MORDELET, E. Articles by GRANON, S.

Histopathological and cognitive defects induced by Nef in the brain

ELODIE MORDELET¹, KARIMA KISSA^*, ARNAUD CRESSANT, FRANCOISE GRAY, SIMONA OZDEN, CATHERINE VIDAL^||, PIERRE CHARNEAU^** and SYLVIE GRANON

Unité Postulante "Mycologie Moléculaire," Institut Pasteur, Paris, France;
^* Unité Postulante "Macrophages et Développement de l’Immunité," Institut Pasteur, Paris, France,
Unité "Rétrovirus & Transfert génétique," Institut Pasteur, Paris, France;
Service d’Anatomie et de Cytologie Pathologiques, Hôpital Lariboisiere, Paris, France;
Unité "Epidémiologie & Physiopathologie des Virus Oncogènes," Institut Pasteur, Paris, France;
^|| CEA, Service de Neurologie, CRSSA, EPHE, Fontenay-aux-Roses, France;
^** Groupe "Virologie Moléculaire & Vectorologie," Institut Pasteur, Paris, France; and
Unité "Récepteurs & Cognition," Institut Pasteur, Paris, France

¹ Correspondence: Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, France. E-mail:mordelet{at}pasteur.fr; or granon{at}pasteur.fr

Complex mechanisms of human immunodeficiency virus type-1 (HIV-1) brain pathogenesis suggest the contribution of individual HIV-1 gene products. Among them, the Nef protein has been reported to harbor a major determinant of pathogenicity in AIDS-like disease. The goal of the present study was to determine whether Nef protein expressed in vivo by primary macrophages could induce a brain toxicity also affecting the behavior of the rat. To achieve this goal we grafted Nef-transduced macrophages into the rat hippocampus. Two months post-transplantation, we observed that Nef induces monocyte/macrophage recruitment, expression of TNF-, and astrogliosis. No apoptotic event was detected. We further demonstrated that Nef neurotoxicity is associated with cognitive deficits.—Mordelet, E., Kissa, K., Cressant, A., Gray, F., Ozden, S., Vidal, C., Charneau, P., Granon, S. Histopathological and cognitive defects induced by Nef in the brain.

Key Words: neuropathology • behavior • bone marrow-derived macrophages

This article has been cited by other articles:

				D. Hoffmann, J. Seebach, A. Cosma, F. D. Goebel, K. Strimmer, H. M. Schatzl, and V. Erfle Therapeutic vaccination reduces HIV sequence variability FASEB J, February 1, 2008; 22(2): 437 - 444. [Abstract] [Full Text] [PDF]