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A novel mechanism of regulation of cardiac contractility by mitochondrial functional state

ALLEN KAASIK^*,, FREDERIC JOUBERT^*, RENÉE VENTURA-CLAPIER^* and VLADIMIR VEKSLER^*,1

^* U-446 INSERM, Laboratoire de Cardiologie Cellulaire et Moléculaire, Faculté de Pharmacie, Université Paris-Sud, Châtenay-Malabry, France; and
Department of Pharmacology, Centre of Molecular and Clinical Medicine, University of Tartu, Estonia

¹Correspondence: U-446 INSERM, Faculté de Pharmacie, Université Paris-Sud, 5 rue J-B Clément, 92296 Châtenay-Malabry, France. E-mail: Vladimir.VEKSLER{at}cep.u-psud.fr

It is generally considered that mitochondria regulate cardiac cell contractility by providing ATP for cellular ATPases and by participating in Ca²⁺ homeostasis. However, other possible mechanisms by which mitochondria can influence contractility have been largely overlooked. Here, we demonstrate that inhibition of the mitochondrial electron transport chain strongly increases Ca²⁺-dependent and independent isometric force development in rat ventricular fibers with selectively permeabilized sarcolemma. This effect is unrelated to the ATP-generating activity of mitochondria or Ca²⁺ homeostasis. Furthermore, various conditions that increase K⁺ accumulation in the mitochondrial matrix (activation of ATP- or Ca²⁺-dependent K⁺ channels as well as inhibition of the K⁺ efflux pathway via the K⁺/H⁺ exchanger) induce a similar mechanical response. Modulators of mitochondrial function that augment isometric force also cause swelling of mitochondria in the vicinity of myofibrils in situ, as shown by confocal microscopy. Osmotic compression of intracellular structures abolishes the effect of mitochondria-induced force modulation, suggesting a mechanical basis for the interaction between the organelles. These findings suggest a novel mechanism for cellular regulation of myofibrillar function, whereby increases in mitochondrial volume can impose mechanical constraints inside the cell, leading to an increase in force developed by myofibrils.—Kaasik, A., Joubert, F., Ventura-Clapier, R., Veksler, V. A novel mechanism of regulation of cardiac contractility by mitochondrial functional state.

Key Words: myocardial contractility • mitochondria • myofibrils • potassium

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