FASEB J. Pierce now sold as Thermo Scientific
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LEE, H. J.
Right arrow Articles by KOH, G. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LEE, H. J.
Right arrow Articles by KOH, G. Y.
(The FASEB Journal. 2004;18:1200-1208.)
© 2004 FASEB

Biological characterization of angiopoietin-3 and angiopoietin-4

HYUEK JONG LEE, CHUNG-HYUN CHO, SU-JEONG HWANG, HAN-HO CHOI, KYUNG-TAE KIM, SO YOUNG AHN, JU-HYUN KIM, JONG-LARK OH, GYUN MIN LEE and GOU YOUNG KOH1

Biomedical Research Center and Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea

1Correspondence: Biomedical Research Center and Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Daejeon, 305-701, Republic of Korea. E-mail: gykoh{at}kaist.ac.kr

The angiopoietin (Ang) family of growth factors includes Ang1, Ang2, Ang3, and Ang4, all of which bind to the endothelial receptor tyrosine kinase Tie2. Ang3 (mouse) and Ang4 (human) are interspecies orthologs. In experiments with human endothelial cell lines, Ang3 was identified as an antagonist of Tie2 and Ang4 was identified as an agonist of Tie2. However, the biological roles of Ang3 and Ang4 are unknown. We examined the biological effect of recombinant Ang3 and Ang4 proteins in primary cultured endothelial cells and in vivo in mice. Recombinant Ang3 and Ang4 formed disulfide-linked dimers. Ang4 (400 ng/mL) markedly increased Tie2 and Akt phosphorylation in primary cultured HUVECs whereas Ang3 (400 ng/mL) did not produce significant changes. Accordingly, Ang4, but not Ang3, induced survival and migration in primary cultured HUVECs. Unexpectedly, intravenously administered Ang3 (30 µg) was more potent than Ang4 (30 µg) in phosphorylating the Tie2 receptor in lung tissue from mice in vivo. Accordingly, Ang3 was more potent than Ang4 in phosphorylating Akt in primary cultured mouse lung microvascular endothelial cells. Ang3 and Ang4 both produced potent corneal angiogenesis extending from the limbus across the mouse cornea in vivo. Thus, Ang3 and Ang4 are agonists of Tie2, but mouse Ang3 has strong activity only on endothelial cells of its own species.—Lee, H. J., Cho, C.-H., Hwang, S.-J., Choi, H.-H., Kim, K.-T., Ahn, S. Y., Kim, J.-H., Oh, J.-L., Lee, G. M., Koh, G. Y. Biological characterization of angiopoietin-3 and angiopoietin-4.


Key Words: Ang3 • Ang4 • Ang1 • Tie2




This article has been cited by other articles:


Home page
 DevelopmentHome page
M. J. Laws, R. N. Taylor, N. Sidell, F. J. DeMayo, J. P. Lydon, D. E. Gutstein, M. K. Bagchi, and I. C. Bagchi
Gap junction communication between uterine stromal cells plays a critical role in pregnancy-associated neovascularization and embryo survival
Development, August 1, 2008; 135(15): 2659 - 2668.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Mofarrahi, T. Nouh, S. Qureshi, L. Guillot, D. Mayaki, and S. N. A. Hussain
Regulation of angiopoietin expression by bacterial lipopolysaccharide
Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L955 - L963.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
H. T. Yuan, S. Venkatesha, B. Chan, U. Deutsch, T. Mammoto, V. P. Sukhatme, A. S. Woolf, and S. A. Karumanchi
Activation of the orphan endothelial receptor Tie1 modifies Tie2-mediated intracellular signaling and cell survival
FASEB J, October 1, 2007; 21(12): 3171 - 3183.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
W. S.N. Shim, I. A.W. Ho, and P. E.H. Wong
Angiopoietin: A TIE(d) Balance in Tumor Angiogenesis
Mol. Cancer Res., July 1, 2007; 5(7): 655 - 665.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
K. Eun Kim, C.-H. Cho, H.-Z. Kim, P. Baluk, D. M. McDonald, and G. Young Koh
In Vivo Actions of Angiopoietins on Quiescent and Remodeling Blood and Lymphatic Vessels in Mouse Airways and Skin
Arterioscler. Thromb. Vasc. Biol., March 1, 2007; 27(3): 564 - 570.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Y. Sato and H. Sonoda
The Vasohibin Family: A Negative Regulatory System of Angiogenesis Genetically Programmed in Endothelial Cells
Arterioscler. Thromb. Vasc. Biol., January 1, 2007; 27(1): 37 - 41.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. R. Macdonald, P. Progias, B. Ciani, S. Patel, U. Mayer, M. O. Steinmetz, and R. A. Kammerer
Structure of the Extracellular Domain of Tie Receptor Tyrosine Kinases and Localization of the Angiopoietin-binding Epitope
J. Biol. Chem., September 22, 2006; 281(38): 28408 - 28414.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
S M Hyder
Sex-steroid regulation of vascular endothelial growth factor in breast cancer.
Endocr. Relat. Cancer, September 1, 2006; 13(3): 667 - 687.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
W. Bureau, P. Van Slyke, J. Jones, R. N. N. Han, N. L. Ward, D. J. Stewart, and D. J. Dumont
Chronic systemic delivery of angiopoietin-2 reveals a possible independent angiogenic effect
Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H948 - H956.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
T. Shibuya, K. Watanabe, H. Yamashita, K. Shimizu, H. Miyashita, M. Abe, T. Moriya, H. Ohta, H. Sonoda, T. Shimosegawa, et al.
Isolation and Characterization of Vasohibin-2 as a Homologue of VEGF-Inducible Endothelium-Derived Angiogenesis Inhibitor Vasohibin
Arterioscler. Thromb. Vasc. Biol., May 1, 2006; 26(5): 1051 - 1057.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
N. P.J. Brindle, P. Saharinen, and K. Alitalo
Signaling and Functions of Angiopoietin-1 in Vascular Protection
Circ. Res., April 28, 2006; 98(8): 1014 - 1023.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
F. Roviezzo, S. Tsigkos, A. Kotanidou, M. Bucci, V. Brancaleone, G. Cirino, and A. Papapetropoulos
Angiopoietin-2 Causes Inflammation in Vivo by Promoting Vascular Leakage
J. Pharmacol. Exp. Ther., August 1, 2005; 314(2): 738 - 744.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. Xu and R. L. Stouffer
Local Delivery of Angiopoietin-2 into the Preovulatory Follicle Terminates the Menstrual Cycle in Rhesus Monkeys
Biol Reprod, June 1, 2005; 72(6): 1352 - 1358.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K.-T. Kim, H.-H. Choi, M. O. Steinmetz, B. Maco, R. A. Kammerer, S. Y. Ahn, H.-Z. Kim, G. M. Lee, and G. Y. Koh
Oligomerization and Multimerization Are Critical for Angiopoietin-1 to Bind and Phosphorylate Tie2
J. Biol. Chem., May 20, 2005; 280(20): 20126 - 20131.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
P. Saharinen, K. Kerkela, N. Ekman, M. Marron, N. Brindle, G. M. Lee, H. Augustin, G. Y. Koh, and K. Alitalo
Multiple angiopoietin recombinant proteins activate the Tie1 receptor tyrosine kinase and promote its interaction with Tie2
J. Cell Biol., April 25, 2005; 169(2): 239 - 243.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by The Federation of American Societies for Experimental Biology.