FASEB J. Mp Biomedicals
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow Supplemental Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ZHAO, M.
Right arrow Articles by McCAIG, C. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ZHAO, M.
Right arrow Articles by McCAIG, C. D.
(The FASEB Journal. 2003;17:397-406.)
© 2003 FASEB

Direct visualization of a stratified epithelium reveals that wounds heal by unified sliding of cell sheets

MIN ZHAO12, BING SONG2, JIN PU, JOHN V. FORRESTER* and COLIN D. McCAIG

Departments of Biomedical Sciences and
* Ophthalmology, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, UK

1Correspondence: Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, UK. E-mail: m.zhao{at}abdn.ac.uk or c.mccaig{at}abdn.ac.uk

Observing cells in their original niche is a key link between the information gleaned from planar culture and in vivo physiology and pathology. A new approach combining the transparency of the cornea, Hoffman modulation optics, and digital imaging allowed movements of individual corneal cells to be viewed directly in situ. 3-Dimensional time-lapse movies imaging unstained cells within the stratified corneal epithelium during wound healing were made. Tracking cell movements dynamically provided a definitive answer to the long-standing question: does a stratified epithelium heal by "sliding" of cell sheets as a coherent unit or do individual cells "leap frog" each other at the wound margin? A wound in the corneal epithelium healed primarily by sliding of the whole epithelium, with ~95% of cells moving with similar speed and trajectories and with little change in their relative position. Only 5% of cells changed layers, with equal proportions moving up or down. Epithelial healing in situ occurred in three phases: a latency, migration, and reconstruction phase. This model provides a unique system to study the behaviors of individual cells in their original niche. It shows that cells slide into a wound as a unified unit to heal a stratified epithelium.—Zhao, M., Song, B., Pu, J., Forrester, J. V., McCaig, C. D. Direct visualization of a stratified epithelium reveals that wounds heal by unified sliding of cell sheets.


Key Words: wound healing • stratified epithelium • corneal epithelium • corneal organ culture • cell migration




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
P. Chen, J. K. McGuire, R. C. Hackman, K.-H. Kim, R. A. Black, K. Poindexter, W. Yan, P. Liu, A. J. Chen, W. C. Parks, et al.
Tissue Inhibitor of Metalloproteinase-1 Moderates Airway Re-Epithelialization by Regulating Matrilysin Activity
Am. J. Pathol., May 1, 2008; 172(5): 1256 - 1270.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
P. Carrier, A. Deschambeault, M. Talbot, C. J. Giasson, F. A. Auger, S. L. Guerin, and L. Germain
Characterization of Wound Reepithelialization Using a New Human Tissue-Engineered Corneal Wound Healing Model
Invest. Ophthalmol. Vis. Sci., April 1, 2008; 49(4): 1376 - 1385.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Z. Li, A. R. Burns, R. E. Rumbaut, and C. W. Smith
{gamma}{delta} T Cells Are Necessary for Platelet and Neutrophil Accumulation in Limbal Vessels and Efficient Epithelial Repair after Corneal Abrasion
Am. J. Pathol., September 1, 2007; 171(3): 838 - 845.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. J. Shanley, P. Walczysko, M. Bain, D. J. MacEwan, and M. Zhao
Influx of extracellular Ca2+ is necessary for electrotaxis in Dictyostelium
J. Cell Sci., November 15, 2006; 119(22): 4741 - 4748.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Z. Li, R. E. Rumbaut, A. R. Burns, and C. W. Smith
Platelet Response to Corneal Abrasion Is Necessary for Acute Inflammation and Efficient Re-epithelialization
Invest. Ophthalmol. Vis. Sci., November 1, 2006; 47(11): 4794 - 4802.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Z. Li, A. R. Burns, and C. W. Smith
Lymphocyte Function-Associated Antigen-1-Dependent Inhibition of Corneal Wound Healing
Am. J. Pathol., November 1, 2006; 169(5): 1590 - 1600.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Z. Li, A. R. Burns, and C. W. Smith
Two Waves of Neutrophil Emigration in Response to Corneal Epithelial Abrasion: Distinct Adhesion Molecule Requirements
Invest. Ophthalmol. Vis. Sci., May 1, 2006; 47(5): 1947 - 1955.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
C. D. McCaig, A. M. Rajnicek, B. Song, and M. Zhao
Controlling Cell Behavior Electrically: Current Views and Future Potential
Physiol Rev, July 1, 2005; 85(3): 943 - 978.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Farooqui and G. Fenteany
Multiple rows of cells behind an epithelial wound edge extend cryptic lamellipodia to collectively drive cell-sheet movement
J. Cell Sci., January 1, 2005; 118(1): 51 - 63.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by The Federation of American Societies for Experimental Biology.