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Mucosal pentraxin (Mptx), a novel rat gene 10-fold down-regulated in colon by dietary heme

CINDY VAN DER MEER-VAN KRAAIJ^*,, ESTHER M. M. VAN LIESHOUT^*,, EVELIEN KRAMER^*,, ROELOF VAN DER MEER^*, and JAAP KEIJER^*,,1

^* Wageningen Centre for Food Sciences (WCFS),
RIKILT, Institute of Food Safety, 6700 AE, Wageningen; and
NIZO Food Research, 6710 BA, Ede, the Netherlands.

¹Correspondence: WCFS, Nutrition and Health, Bornsesteeg 45, P.O box 230, 6700 AE, Wageningen, the Netherlands. E-mail: jaap.keijer{at}wur.nl

Consumption of red meat is associated with increased colon cancer risk. Our previous work indicated that this association might be due to the heme content of red meat. In rat studies, dietary heme increased colonic cytotoxicity and epithelial cell turnover, carcinogenesis biomarkers. Here we apply DNA microarray technology to examine effects of heme on colonic gene expression. A rat colon-specific microarray was constructed and hybridized in duplicate to RNA extracts from colon scrapings of rats fed diets with or without heme (n=6–7). We were able to reproducibly identify changes in colonic mRNA abundance in response to heme. Most striking was a >10-fold down-regulation of a single rat gene, an unprecedented gene-modulating effect of a dietary component. Based on homology, the novel gene encodes a pentraxin, the first identified in colon. Pentraxins are postulated to be involved in dealing with dying cells. Quantitative PCR confirmed the strong heme-induced down-regulation of this gene, which we named mucosal pentraxin (Mptx). Overall, our data support the efficacy of cDNA array expression profiling to investigate effects of specific nutrients in an in vivo system and may provide an approach to establishing markers for diet-induced stress of mammalian colonic mucosa.—van der Meer-van Kraaij, C., van Lieshout, E. M. M., Kramer, E., van der Meer, R., Keijer, J. Mucosal pentraxin (Mptx), a novel rat gene 10-fold down-regulated in colon by dietary heme.

Key Words: red meat • microarray • colon cancer

This article has been cited by other articles:

				C. van der Meer-van Kraaij, E. Kramer, D. Jonker-Termont, M.B. Katan, R. van der Meer, and J. Keijer Differential gene expression in rat colon by dietary heme and calcium Carcinogenesis, January 1, 2005; 26(1): 73 - 79. [Abstract] [Full Text] [PDF]