|
|
||||||||
HELIOS Klinikum Berlin, Franz Volhard Clinic and Max Delbrück Center for Molecular Medicine, Medical Faculty of the Charité, Humboldt University of Berlin, Germany
2Correspondence: Franz-Volhard Klinik, Wiltbergstrasse 50, 13125 Berlin, Germany. E-mail: sharma@fvk-berlin.de or gollasch{at}fvk-berlin.de
Virtually all blood vessels are surrounded by adventitial fat. Adipocytes produce a host of vasoactive substances that may influence vascular contraction. We tested whether or not perivascular adipose tissue modulates contraction of aortic ring preparations. We studied aortic rings surrounded by periadventitial adipose tissue from adult Sprague-Dawley rats. At a maximum concentration of 300 nM angiotensin II, 6.5 µM serotonin, and 5 µM phenylephrine, the contractile response of intact rings was 95%, 80%, and 30% lower than that of vessels without periadventitial fat. The anticontractile effect of periadventitial fat was reduced by inhibition of ATP-dependent K+ channels with glibenclamide (3 µM) and by the tyrosine kinase inhibitor genistein (10 µM). Blocking NOS, cyclo-oxygenase, cytochrome P450, or adenosine receptors did not restore the vascular response in intact vessels. The anticontractile effect of perivascular fat was present in Zucker fa/fa rats, suggesting that leptin receptors were not responsible. Transferring the bath solution from intact vessels, isolated periadventitial tissue, and cultured rat adipocytes to precontracted vessels lacking periadventitial fat resulted in a rapid relaxation. We suggest that perivascular adventitial adipose tissue releases a transferable adventitium-derived relaxing factor that acts by tyrosine kinase-dependent activation of K+ channels in vascular smooth muscle cells.Löhn, M., Dubrovska, G., Lauterbach, B., Luft, F. C., Gollasch, M., Sharma, A. M. Periadventitial fat releases a vascular relaxing factor.
Key Words: fat adventitium ion channels tyrosine phosphorylation
This article has been cited by other articles:
![]() |
G. A. Molnar, C. Lindschau, G. Dubrovska, P. R. Mertens, T. Kirsch, M. Quinkler, M. Gollasch, S. Wresche, F. C. Luft, D. N. Muller, et al. Glucocorticoid-Related Signaling Effects in Vascular Smooth Muscle Cells Hypertension, May 1, 2008; 51(5): 1372 - 1378. [Abstract] [Full Text] [PDF] |
||||
![]() |
B Galvez-Prieto, J Bolbrinker, P Stucchi, A I de las Heras, B Merino, S Arribas, M Ruiz-Gayo, M Huber, M Wehland, R Kreutz, et al. Comparative expression analysis of the renin-angiotensin system components between white and brown perivascular adipose tissue J. Endocrinol., April 1, 2008; 197(1): 55 - 64. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. A. Rosito, J. M. Massaro, U. Hoffmann, F. L. Ruberg, A. A. Mahabadi, R. S. Vasan, C. J. O'Donnell, and C. S. Fox Pericardial Fat, Visceral Abdominal Fat, Cardiovascular Disease Risk Factors, and Vascular Calcification in a Community-Based Sample: The Framingham Heart Study Circulation, February 5, 2008; 117(5): 605 - 613. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Malinowski, M. A. Deja, K. S. Golba, T. Roleder, J. Biernat, and S. Wos Perivascular tissue of internal thoracic artery releases potent nitric oxide and prostacyclin-independent anticontractile factor Eur. J. Cardiothorac. Surg., February 1, 2008; 33(2): 225 - 231. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. A. Auger, P. D'Orleans-Juste, and L. Germain Adventitia contribution to vascular contraction: Hints provided by tissue-engineered substitutes Cardiovasc Res, September 1, 2007; 75(4): 669 - 678. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Thalmann and C. A. Meier Local adipose tissue depots as cardiovascular risk factors Cardiovasc Res, September 1, 2007; 75(4): 690 - 701. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Fesus, G. Dubrovska, K. Gorzelniak, R. Kluge, Y. Huang, F. C. Luft, and M. Gollasch Adiponectin is a novel humoral vasodilator Cardiovasc Res, September 1, 2007; 75(4): 719 - 727. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. Takemori, Y.-J. Gao, L. Ding, C. Lu, L.-Y. Su, W.-S. An, C. Vinson, and R. M.K.W. Lee Elevated Blood Pressure in Transgenic Lipoatrophic Mice and Altered Vascular Function Hypertension, February 1, 2007; 49(2): 365 - 372. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. U. Momin, N. Melikian, A. M. Shah, D. J. Grieve, S. B. Wheatcroft, L. John, A. El Gamel, J. B. Desai, T. Nelson, C. Driver, et al. Leptin is an endothelial-independent vasodilator in humans with coronary artery disease: evidence for tissue specificity of leptin resistance Eur. Heart J., October 1, 2006; 27(19): 2294 - 2299. [Abstract] [Full Text] [PDF] |
||||
![]() |
V. Lamounier-Zepter, M. Ehrhart-Bornstein, P. Karczewski, H. Haase, S. R. Bornstein, and I. Morano Human adipocytes attenuate cardiomyocyte contraction: characterization of an adipocyte-derived negative inotropic activity FASEB J, August 1, 2006; 20(10): 1653 - 1659. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y.-J. Gao, K. Takemori, L.-Y. Su, W.-S. An, C. Lu, A. M. Sharma, and R. M.K.W. Lee Perivascular adipose tissue promotes vasoconstriction: the role of superoxide anion Cardiovasc Res, July 15, 2006; 71(2): 363 - 373. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. Galvez, J. de Castro, D. Herold, G. Dubrovska, S. Arribas, M. C. Gonzalez, I. Aranguez, F. C. Luft, M. P. Ramos, M. Gollasch, et al. Perivascular Adipose Tissue and Mesenteric Vascular Function in Spontaneously Hypertensive Rats Arterioscler. Thromb. Vasc. Biol., June 1, 2006; 26(6): 1297 - 1302. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. Henrichot, C. E. Juge-Aubry, A. Pernin, J.-C. Pache, V. Velebit, J.-M. Dayer, P. Meda, C. Chizzolini, and C. A. Meier Production of Chemokines by Perivascular Adipose Tissue: A Role in the Pathogenesis of Atherosclerosis? Arterioscler. Thromb. Vasc. Biol., December 1, 2005; 25(12): 2594 - 2599. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Engeli Is there a pathophysiological role for perivascular adipocytes? Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H1794 - H1795. [Full Text] [PDF] |
||||
![]() |
C. Barandier, J.-P. Montani, and Z. Yang Mature adipocytes and perivascular adipose tissue stimulate vascular smooth muscle cell proliferation: effects of aging and obesity Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H1807 - H1813. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. D. Rudic, P. McNamara, D. Reilly, T. Grosser, A.-M. Curtis, T. S. Price, S. Panda, J. B. Hogenesch, and G. A. FitzGerald Bioinformatic Analysis of Circadian Gene Oscillation in Mouse Aorta Circulation, October 25, 2005; 112(17): 2716 - 2724. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y.-J. Gao, Z.-h. Zeng, K. Teoh, A. M. Sharma, L. Abouzahr, I. Cybulsky, A. Lamy, L. Semelhago, and R. M.K.W. Lee Perivascular adipose tissue modulates vascular function in the human internal thoracic artery J. Thorac. Cardiovasc. Surg., October 1, 2005; 130(4): 1130 - 1136. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Dietrich, M. Mederos y Schnitzler, M. Gollasch, V. Gross, U. Storch, G. Dubrovska, M. Obst, E. Yildirim, B. Salanova, H. Kalwa, et al. Increased Vascular Smooth Muscle Contractility in TRPC6-/- Mice Mol. Cell. Biol., August 15, 2005; 25(16): 6980 - 6989. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Crowley, S. Gurley, M. Oliverio, A. Pazmino, R Griffiths, P. Flannery, R. Spurney, H-S Kim, O Smithies, T. Le, et al. Is the Kidney Always the Cause of Hypertension?: Distinct Roles for the Kidney and Systemic Tissues in Blood Pressure Regulation by the Renin-Angiotensin System. J Clin Invest 115: 1092-1099, 2005 J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1525 - 1532. [Full Text] [PDF] |
||||
![]() |
K. M. Gauthier, D. V. Baewer, S. Hittner, C. J. Hillard, K. Nithipatikom, D. S. Reddy, J. R. Falck, and W. B. Campbell Endothelium-derived 2-arachidonylglycerol: an intermediate in vasodilatory eicosanoid release in bovine coronary arteries Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1344 - H1351. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. S. Fernandez-Alfonso Regulation of Vascular Tone: The Fat Connection Hypertension, September 1, 2004; 44(3): 255 - 256. [Full Text] [PDF] |
||||
![]() |
S. Verlohren, G. Dubrovska, S.-Y. Tsang, K. Essin, F. C. Luft, Y. Huang, and M. Gollasch Visceral Periadventitial Adipose Tissue Regulates Arterial Tone of Mesenteric Arteries Hypertension, September 1, 2004; 44(3): 271 - 276. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Dubrovska, S. Verlohren, F. C. Luft, and M. Gollasch Mechanisms of ADRF release from rat aortic adventitial adipose tissue Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H1107 - H1113. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |