| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Istituto Pasteur Fondazione Cenci Bolognetti, Department of Histology and Medical Embryology, University of Rome ‘La Sapienza’, 00161 Rome, Italy;
* Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK;
Molecular Medicine Section, Department of Neuroscience, University of Siena, 53100 Siena, and DIBIT S. Raffaele Scientific Institute, 20132 Milano, Italy; and
Department of Pharmacology, University of Cambridge, Cambridge, CB2 1QJ, UK
1Correspondence: Department of Histology and Medical Embryology, University of Rome ‘La Sapienza’, Via A. Scarpa, 14, 00161 Rome, Italy. E-mail: antonio.filippini{at}uniroma1.it
cADPR, a potent calcium-mobilizing intracellular messenger synthesized by ADP-ribosyl cyclases regulates openings of ryanodine receptors (RyR). Here we report that in the rat testis, a functional cADPR Ca2+ release system is essential for the contractile response of peritubular smooth muscle cells (PSMC) to endothelin (ET). We previously showed that this potent smooth muscle agonist elicits intracellular Ca2+ release in PSMC and seminiferous tubule contraction via activation of ETA and ETB receptors. ETB-R induces the mobilization of a thapsigargin-sensitive but IP3-independent intracellular Ca2+ pool. Stimulation of permeabilized PSMC with cADPR was found to elicit large Ca2+ releases blocked by either a selective antagonist of cADPR or a RyR blocker, but not by heparin. Western blotting and confocal fluorescence microscopy indicated the specific expression of type 2 RyR in perinuclear localization. ET was found to stimulate the activity of ADP-ribosyl cyclase. Microinjection of the selective cADPR antagonist 8NH2-cADPR completely abolished subsequent stimulation of Ca2+ signaling via ETA and ETB receptors. cADPR therefore appears to have an obligatory role for ETA-R and ETB-R-mediated calcium signaling in PSMC. However, ETB-R seem to be coupled exclusively to cADPR whereas ETA-R activation may be linked to IP3 and cADPR signaling pathways.—Barone, F., Genazzani, A. A., Conti, A., Churchill, G. C., Palombi, F., Ziparo, E., Sorrentino, V., Galione, A., Filippini, A. A pivotal role for cADPR-mediated Ca2+ signaling: regulation of endothelin-induced contraction in peritubular smooth muscle cells.
Key Words: ryanodine receptors • calcium signaling • ADP-ribosyl cyclase • seminiferous tubule
This article has been cited by other articles:
|
|
 |
|
  T. L. Thai and W. J. Arendshorst ADP-ribosyl cyclase and ryanodine receptors mediate endothelin ETA and ETB receptor-induced renal vasoconstriction in vivo Am J Physiol Renal Physiol, August 1, 2008; 295(2): F360 - F368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Malavasi, S. Deaglio, A. Funaro, E. Ferrero, A. L. Horenstein, E. Ortolan, T. Vaisitti, and S. Aydin Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology Physiol Rev, July 1, 2008; 88(3): 841 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Jude, M. E. Wylam, T. F. Walseth, and M. S. Kannan Calcium Signaling in Airway Smooth Muscle Proceedings of the ATS, January 1, 2008; 5(1): 15 - 22. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Fellner and W. Arendshorst Endothelin-A and -B receptors, superoxide, and Ca2+ signaling in afferent arterioles Am J Physiol Renal Physiol, January 1, 2007; 292(1): F175 - F184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Deshpande, T. A. White, S. Dogan, T. F. Walseth, R. A. Panettieri, and M. S. Kannan CD38/cyclic ADP-ribose signaling: role in the regulation of calcium homeostasis in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L773 - L788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Fellner and L. Parker Endothelin-1, superoxide and adeninediphosphate ribose cyclase in shark vascular smooth muscle J. Exp. Biol., March 15, 2005; 208(6): 1045 - 1052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Deshpande, T. A. White, A. G. P. Guedes, C. Milla, T. F. Walseth, F. E. Lund, and M. S. Kannan Altered Airway Responsiveness in CD38-Deficient Mice Am. J. Respir. Cell Mol. Biol., February 1, 2005; 32(2): 149 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Laporte, A. Hui, and I. Laher Pharmacological Modulation of Sarcoplasmic Reticulum Function in Smooth Muscle Pharmacol. Rev., December 1, 2004; 56(4): 439 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Fellner and L. A. Parker Endothelin B receptor Ca2+ signaling in shark vascular smooth muscle: participation of inositol trisphosphate and ryanodine receptors J. Exp. Biol., September 1, 2004; 207(19): 3411 - 3417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chiarella, R. Puglisi, V. Sorrentino, C. Boitani, and M. Stefanini Ryanodine receptors are expressed and functionally active in mouse spermatogenic cells and their inhibition interferes with spermatogonial differentiation J. Cell Sci., August 15, 2004; 117(18): 4127 - 4134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Deshpande, S. Dogan, T. F. Walseth, S. M. Miller, Y. Amrani, R. A. Panettieri, and M. S. Kannan Modulation of Calcium Signaling by Interleukin-13 in Human Airway Smooth Muscle: Role of CD38/Cyclic Adenosine Diphosphate Ribose Pathway Am. J. Respir. Cell Mol. Biol., July 1, 2004; 31(1): 36 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Just, A. J. M. Olson, and W. J. Arendshorst Dual constrictor and dilator actions of ETB receptors in the rat renal microcirculation: interactions with ETA receptors Am J Physiol Renal Physiol, April 1, 2004; 286(4): F660 - F668. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
