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1
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Department of Medicine B, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany;
Central Ultrastructure Research Unit, Interdisciplinary Centre of Clinical Research, c/o Gerhard Domagk Institute of Pathology, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany;
Experimental Cardiology, Max Planck Institute for Clinical Research, D-61231 Bad Nauheim, Germany;
Gerhard Domagk Institute of Pathology, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany;
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Franz Volhard Clinic, Medical Faculty of the Charite, Humboldt University of Berlin, D-13125 Berlin, Germany; and
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Institute for Arteriosclerosis Research, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany
1Correspondence: Department of Medicine B and Central Ultrastructure Research Unit, Interdisciplinary Centre of Clinical Research, c/o Gerhard Domagk Institute of Pathology, Westfälische Wilhelms-Universität Münster, Domagkstr. 17, D-48149 Münster, Germany. E-mail: buchwalo{at}uni-muenster.de
The concept of endothelium-derived relaxing factor (EDRF) put forward in 1980 by Furchgott and Zawadzki implies that nitric oxide (NO) produced by NO synthase (NOS) in the endothelium diffuses to the underlying vascular smooth muscle, where it modulates vascular tone as well as vascular smooth muscle cell (VSMC) proliferation by increasing cGMP formation with subsequent activation of cGMP-dependent protein kinase. According to this concept, VSMC do not express NOS by themselves. This attractive, simple scheme is now under considerable debate. To address this issue, we designed this study with the use of a novel supersensitive immunocytochemical technique of signal amplification with tyramide and electron microscopic immunogold labeling complemented with Western blotting, as in our recent studies demonstrating NOS in the myocardial and skeletal muscles. We provide the first evidence that, in contrast to the currently accepted view, VSMC in various blood vessels express all three NOS isoforms depending on the blood vessel type. These findings suggest an alternative mechanism by which local NOS expression may modulate vascular functions in an endothelium-independent manner.—Buchwalow, I. B., Podzuweit, T., Böcker, W., Samoilova, V. E., Thomas S., Wellner, M., Baba, H. A., Robenek, H., Schnekenburger, J., Lerch, M. M. Vascular smooth muscle and nitric oxide synthase.
Key Words: vascular smooth muscle cells • NOS • VSMC
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