| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institute of Public Health, University of Copenhagen, Denmark
1Correspondence: Institute of Public Health, c/o Department of Pharmacology, The Panum Institute, Bldg. 18.5, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. E-mail: s.loft{at}pubhealth.ku.dk
Estrogenic chemicals are suspected of affecting cancer risk and male reproduction, possibly involving oxidative DNA damage. In this study, formation of 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxodG), was measured in testicular cells from rats after 17 
-ethinylestradiol (EE) exposure in vivo and in vitro after incubation with EE with or without an antiestrogen. In vivo, preadult (30–35 days) and adult (110–120 days) Wistar rats received 0, 2.8, or 56 mg EE/kg body weight as intraperitoneal injections (n=6). After 1 or 4 h, the 8-oxodG/106 dG ratio was measured in the liver, kidneys, and testes. Testes DNA analysis revealed an age-related effect (adult animals had a higher ratio than the young animals) and a concentration effect in preadult rats (increased EE-concentration caused increased ratio), but no time effect. No differences were found in the liver or kidneys. In vitro, testicular cells were isolated and incubated with EE concentrations ranging from 0.1 to 1000 nM. The results indicated an increase in 8-oxodG/106 dG from 0 to 10 nM estrogen. At 1000 nM, the level was close to control level. Coincubation of 10 nM EE (maximum damage) with an estrogen antagonist, ICI 182.780, abolished the effect at 10 nM, indicating that the damaging effect is estrogen receptor mediated.—Wellejus, A., Loft, S. Receptor-mediated ethinylestradiol-induced oxidative DNA damage in rat testicular cells.
Key Words: 7,8-dihydro-8-oxo-2'-deoxyguanosine • glutathione • 17-ethinylestradiol
This article has been cited by other articles:
|
|
 |
|
  A. Wellejus, A. Olsen, A. Tjonneland, B. L. Thomsen, K. Overvad, and S. Loft Urinary Hydroxyestrogens and Breast Cancer Risk among Postmenopausal Women: A Prospective Study Cancer Epidemiol. Biomarkers Prev., September 1, 2005; 14(9): 2137 - 2142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Risom, M. Dybdahl, J. Bornholdt, U. Vogel, H. Wallin, P. Moller, and S. Loft Oxidative DNA damage and defence gene expression in the mouse lung after short-term exposure to diesel exhaust particles by inhalation Carcinogenesis, November 1, 2003; 24(11): 1847 - 1852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dybdahl, L. Risom, P. Moller, H. Autrup, H. Wallin, U. Vogel, J. Bornholdt, B. Daneshvar, L. O. Dragsted, A. Weimann, et al. DNA adduct formation and oxidative stress in colon and liver of Big Blue(R) rats after dietary exposure to diesel particles Carcinogenesis, November 1, 2003; 24(11): 1759 - 1766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Pais, I. Leav, K.-M. Lau, Z. Jiang, and S.-M. Ho Estrogen Receptor-{beta} Expression in Human Testicular Germ Cell Tumors Clin. Cancer Res., October 1, 2003; 9(12): 4475 - 4482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Moller, H. Wallin, U. Vogel, H. Autrup, L. Risom, M. T. Hald, B. Daneshvar, L. O. Dragsted, H. E. Poulsen, and S. Loft Mutagenicity of 2-amino-3-methylimidazo[4,5-f]quinoline in colon and liver of Big Blue rats: role of DNA adducts, strand breaks, DNA repair and oxidative stress Carcinogenesis, August 1, 2002; 23(8): 1379 - 1385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. O. Dragsted, B. Daneshvar, U. Vogel, H. N. Autrup, H. Wallin, L. Risom, P. Moller, A. M. Molck, M. Hansen, H. E. Poulsen, et al. A Sucrose-rich Diet Induces Mutations in the Rat Colon Cancer Res., August 1, 2002; 62(15): 4339 - 4345. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
