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signal transduction in rat neonatal cardiac myocytes: definition of pathways generating from the TNF- receptor
,
1,2
* Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, USA;
II Faculty of Medicine, University "La Sapienza", 00161, Rome, Italy;
I.R.C.C.S. Neuromed, Pozzilli (IS), Italy;
** Department of Medicine, University of Naples, 80131, Naples, Italy;
# Department of Radiation Oncology, Massey Cancer Center, Medical College of Virginia, Richmond, Virginia, USA;
¶ Centro di Endocrinologia ed Endocrinologia Sperimentale, C.N.R., 80131, Naples, Italy; and
Biotechnology Department, College of Science and Technology, Sbarro Institute for Cancer Research, Temple University, Philadelphia, PA 19122
1Correspondence: Kimmel Cancer Center, 233 S. 10th St., Rm. 1006, Philadelphia PA 19107, USA. E-mail: gianluigi.condorelli{at}mail.tju.edu
Cardiomyocyte hypertrophy and apoptosis have been implicated in the loss of contractile function during heart failure (HF). Moreover, patients with HF have been shown to exhibit increased levels of tumor necrosis factor 
(TNF-) in the myocardium. However, the multiple signal transduction pathways generating from the TNF- receptor in cardiomyocytes and leading preferentially to apoptosis or hypertrophy are still unknown. Here we demonstrate in neonatal rat cardiomyocytes that 1) TNF- induces phosphorylation of AKT, activation of NF-
B, and the phosphorylation of JUN kinase; 2) blocking AKT activity prevents NF-B activation, suggesting a role for AKT in regulating NF-B function; 3) AKT and JUN are both critical for the hypertrophic effects of TNF-, since dominant-negative mutants of these genes are capable of inhibiting TNF--induced ANF-promoter up-regulation and increase in cardiomyocyte cell size, and 4) blocking NF-B, AKT, or JUN alone or in combination does not sensitize cardiomyocytes to the proapoptotic effects of TNF-, in contrast to other cell types, suggesting a cardiac-specific pathway regulating the anti-apoptotic events induced by TNF-. Altogether, the data presented evidence the role of AKT and JUN in TNF--induced cardiomyocyte hypertrophy and apoptosis.—Condorelli, G., Morisco, C., Latronico, M., Claudio, P. P., Dent, P., Tsichlis, P., Condorelli, G., Frati, G., Drusco, A., Croce, C. M., Napoli, C. TNF- signal transduction in rat neonatal cardiac myocytes: definition of pathways generating from the TNF- receptor.
Key Words: cardiomyocyte apoptosis • hypertrophy • AKT • heart failure • tumor necrosis factor
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