| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* Department of Dermatology, Boston University School of Medicine, Boston, MA; and
Department of Dermatology, Columbia University, New York, NY, USA
1Correspondence: Department of Dermatology, Boston University School of Medicine, 609 Albany St., Boston, MA 02118, USA. E-mail: bgilchre{at}bu.edu
Hair graying, an age-associated process of unknown etiology, is characterized by a reduced number and activity of hair follicle (HF) melanocytes. Stem cell factor (SCF) and its receptor c-kit are important for melanocyte survival during development, and mutations in these genes result in unpigmented hairs. Here we show that during cyclic HF regeneration in C57BL/6 mice, proliferating, differentiating, and melanin-producing melanocytes express c-kit, whereas presumptive melanocyte precursors do not. SCF overexpression in HF epithelium significantly increases the number and proliferative activity of melanocytes. During the induced hair cycle in C57BL/6 mice, administration of anti-c-kit antibody dose-dependently decreases hair pigmentation and leads to partially depigmented (gray) or fully depigmented (white) hairs, associated with significant decreases in melanocyte proliferation and differentiation, as determined by immunostaining and confocal microscopy. However, in the next hair cycle, the previously treated animals grow fully pigmented hairs with the normal number and distribution of melanocytes. This suggests that melanocyte stem cells are not dependent on SCF/c-kit and when appropriately stimulated can generate melanogenically active melanocytes. Therefore, the blockade of c-kit signaling offers a fully reversible model for hair depigmentation, which might be used for the studies of hair pigmentation disorders.—Botchkareva, N. V., Khlgatian, M., Longley, B. J., Botchkarev, V. A., and Gilchrest, B. A. SCF/c-kit signaling is required for cyclic regeneration of the hair pigmentation unit.
Key Words: melanocyte • stem cell • hair follicle • hair cycle
This article has been cited by other articles:
|
|
 |
|
  T. Inoue-Narita, K. Hamada, T. Sasaki, S. Hatakeyama, S. Fujita, K. Kawahara, M. Sasaki, H. Kishimoto, S. Eguchi, I. Kojima, et al. Pten Deficiency in Melanocytes Results in Resistance to Hair Graying and Susceptibility to Carcinogen-Induced Melanomagenesis Cancer Res., July 15, 2008; 68(14): 5760 - 5768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A Randall, T. J Jenner, N. A Hibberts, I. O De Oliveira, and T. Vafaee Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles J. Endocrinol., April 1, 2008; 197(1): 11 - 23. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M.J. Peters, S. Liotiri, E. Bodo, E. Hagen, T. Biro, P. C. Arck, and R. Paus Probing the Effects of Stress Mediators on the Human Hair Follicle: Substance P Holds Central Position Am. J. Pathol., December 1, 2007; 171(6): 1872 - 1886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Widakowich, G. de Castro Jr., E. de Azambuja, P. Dinh, and A. Awada Review: Side Effects of Approved Molecular Targeted Therapies in Solid Cancers Oncologist, December 1, 2007; 12(12): 1443 - 1455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lee, J. M. Basak, S. Demehri, and R. Kopan Bi-compartmental communication contributes to the opposite proliferative behavior of Notch1-deficient hair follicle and epidermal keratinocytes Development, August 1, 2007; 134(15): 2795 - 2806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Lai, T. Kuzel, and M. E. Lacouture Hand-Foot and Stump Syndrome to Sorafenib J. Clin. Oncol., January 20, 2007; 25(3): 341 - 343. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Routhouska, A. C. Gilliam, and P. Mirmirani Hair Depigmentation During Chemotherapy With a Class III/V Receptor Tyrosine Kinase Inhibitor. Arch Dermatol, November 1, 2006; 142(11): 1477 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Ohnemus, M. Uenalan, J. Inzunza, J.-A. Gustafsson, and R. Paus The Hair Follicle as an Estrogen Target and Source Endocr. Rev., October 1, 2006; 27(6): 677 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Arad, N. Konnikov, D. A. Goukassian, and B. A. Gilchrest T-oligos augment UV-induced protective responses in human skin FASEB J, September 1, 2006; 20(11): 1895 - 1897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Faivre, C. Delbaldo, K. Vera, C. Robert, S. Lozahic, N. Lassau, C. Bello, S. Deprimo, N. Brega, G. Massimini, et al. Safety, Pharmacokinetic, and Antitumor Activity of SU11248, a Novel Oral Multitarget Tyrosine Kinase Inhibitor, in Patients With Cancer J. Clin. Oncol., January 1, 2006; 24(1): 25 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Osawa, G. Egawa, S.-S. Mak, M. Moriyama, R. Freter, S. Yonetani, F. Beermann, and S.-I. Nishikawa Molecular characterization of melanocyte stem cells in their niche Development, December 15, 2005; 132(24): 5589 - 5599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Sharov, M. Fessing, R. Atoyan, T. Y. Sharova, C. Haskell-Luevano, L. Weiner, K. Funa, J. L. Brissette, B. A. Gilchrest, and V. A. Botchkarev Bone morphogenetic protein (BMP) signaling controls hair pigmentation by means of cross-talk with the melanocortin receptor-1 pathway PNAS, January 4, 2005; 102(1): 93 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Slominski, D. J. Tobin, S. Shibahara, and J. Wortsman Melanin Pigmentation in Mammalian Skin and Its Hormonal Regulation Physiol Rev, October 1, 2004; 84(4): 1155 - 1228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Guyonneau, F. Murisier, A. Rossier, A. Moulin, and F. Beermann Melanocytes and Pigmentation Are Affected in Dopachrome Tautomerase Knockout Mice Mol. Cell. Biol., April 15, 2004; 24(8): 3396 - 3403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Yu, M. Becker-Hapak, E. L. Snyder, M. Vooijs, C. Denicourt, and S. F. Dowdy Distinct and nonoverlapping roles for pRB and cyclin D:cyclin-dependent kinases 4/6 activity in melanocyte survival PNAS, December 9, 2003; 100(25): 14881 - 14886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Moss, G. C. Toner, J. M. Cherrington, D. B. Mendel, and A. D. Laird Hair Depigmentation Is a Biological Readout for Pharmacological Inhibition of KIT in Mice and Humans J. Pharmacol. Exp. Ther., November 1, 2003; 307(2): 476 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bandyopadhyay, N. A. Okan, E. Bales, L. Nascimento, P. A. Cole, and E. E. Medrano Down-Regulation of p300/CBP Histone Acetyltransferase Activates a Senescence Checkpoint in Human Melanocytes Cancer Res., November 1, 2002; 62(21): 6231 - 6239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. J. Peters, D. J. Tobin, N. Botchkareva, M. Maurer, and R. Paus Migration of Melanoblasts into the Developing Murine Hair Follicle Is Accompanied by Transient c-Kit Expression J. Histochem. Cytochem., June 1, 2002; 50(6): 751 - 766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Tobin, K. Foitzik, T. Reinheckel, L. Mecklenburg, V. A. Botchkarev, C. Peters, and R. Paus The Lysosomal Protease Cathepsin L Is an Important Regulator of Keratinocyte and Melanocyte Differentiation During Hair Follicle Morphogenesis and Cycling Am. J. Pathol., May 1, 2002; 160(5): 1807 - 1821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. BOTCHKAREV, N. V. BOTCHKAREVA, M. NAKAMURA, O. HUBER, K. FUNA, R. LAUSTER, R. PAUS, and B. A. GILCHREST Noggin is required for induction of the hair follicle growth phase in postnatal skin FASEB J, October 1, 2001; 15(12): 2205 - 2214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Botchkarev, E. A. Komarova, F. Siebenhaar, N. V. Botchkareva, A. A. Sharov, P. G. Komarov, M. Maurer, A. V. Gudkov, and B. A. Gilchrest p53 Involvement in the Control of Murine Hair Follicle Regression Am. J. Pathol., June 1, 2001; 158(6): 1913 - 1919. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
