FASEB J. Uncover Your Biological Pathway
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LA, M.
Right arrow Articles by PERRETTI, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LA, M.
Right arrow Articles by PERRETTI, M.
(The FASEB Journal. 2001;15:2247-2256.)
© 2001 FASEB

Annexin 1 peptides protect against experimental myocardial ischemia-reperfusion: analysis of their mechanism of action

MYLINH LA1, MICHELE D’AMICO*, SILVIO BANDIERA, CLARA DI FILIPPO*, SONIA M. OLIANI{dagger}, FELICITY N. E. GAVINS, RODERICK J. FLOWER and MAURO PERRETTI

The William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, United Kingdom;
* Department of Experimental Medicine, Section of Pharmacology L. Donatelli, Second University of Naples, 80138 Naples, Italy; and
{dagger} Department of Biology, IBILCE-UNESP, Sao José do Rio Preto, SP, Brazil

1Correspondence: Department of Biochemical Pharmacology, The William Harvey Research Institute, Charterhouse Square, London EC1M 6BQ, United Kingdom. E-mail: m.la{at}mds.qmw.ac.uk

Myocardial reperfusion injury is associated with the infiltration of blood-borne polymorphonuclear leukocytes. We have previous described the protection afforded by annexin 1 (ANXA1) in an experimental model of rat myocardial ischemia-reperfusion (IR) injury. We examined the 1) amino acid region of ANXA1 that retained the protective effect in a model of rat heart IR; 2) changes in endogenous ANXA1 in relation to the IR induced damage and after pharmacological modulation; and 3) potential involvement of the formyl peptide receptor (FPR) in the protective action displayed by ANXA1 peptides. Administration of peptide Ac2–26 at 0, 30, and 60 min postreperfusion produced a significant protection against IR injury, and this was associated with reduced myeloperoxidase activity and IL-1ß levels in the infarcted heart. Western blotting and electron microscopy analyses showed that IR heart had increased ANXA1 expression in the injured tissue, associated mainly with the infiltrated leukocytes. Finally, an antagonist to the FPR receptor selectively inhibited the protective action of peptide ANXA1 and its derived peptides against IR injury. Altogether, these data provide further insight into the protective effect of ANXA1 and its mimetics and a rationale for a clinical use for drugs developed from this line of research.—La, M., D’Amico, M., Bandiera, S., Di Filippo, C., Oliani, S. M., Gavins, F. N. E., Flower, R. J., Perretti, M. Annexin 1 peptides protect against experimental myocardial ischemia-reperfusion: analysis of their mechanism of action.


Key Words: lipocortin 1 • receptor • neutrophil • FPR • fMLP




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
T. S. Gastardelo, A. S. Damazo, J. Dalli, R. J. Flower, M. Perretti, and S. M. Oliani
Functional and Ultrastructural Analysis of Annexin A1 and Its Receptor in Extravasating Neutrophils during Acute Inflammation
Am. J. Pathol., January 1, 2009; 174(1): 177 - 183.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. A. Babbin, M. G. Laukoetter, P. Nava, S. Koch, W. Y. Lee, C. T. Capaldo, E. Peatman, E. A. Severson, R. J. Flower, M. Perretti, et al.
Annexin A1 Regulates Intestinal Mucosal Injury, Inflammation, and Repair
J. Immunol., October 1, 2008; 181(7): 5035 - 5044.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. G. Souza, C. T. Fagundes, F. A. Amaral, D. Cisalpino, L. P. Sousa, A. T. Vieira, V. Pinho, J. R. Nicoli, L. Q. Vieira, I. M. Fierro, et al.
The Required Role of Endogenously Produced Lipoxin A4 and Annexin-1 for the Production of IL-10 and Inflammatory Hyporesponsiveness in Mice
J. Immunol., December 15, 2007; 179(12): 8533 - 8543.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
Y. Birnbaum, Y. Ye, Y. Lin, S. Y. Freeberg, S. P. Nishi, J. D. Martinez, M.-H. Huang, B. F. Uretsky, and J. R. Perez-Polo
Augmentation of Myocardial Production of 15-Epi-Lipoxin-A4 by Pioglitazone and Atorvastatin in the Rat
Circulation, August 29, 2006; 114(9): 929 - 935.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. P. G. Hayhoe, A. M. Kamal, E. Solito, R. J. Flower, D. Cooper, and M. Perretti
Annexin 1 and its bioactive peptide inhibit neutrophil-endothelium interactions under flow: indication of distinct receptor involvement
Blood, March 1, 2006; 107(5): 2123 - 2130.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
C. Bandeira-Melo, A. G. C. Bonavita, B. L. Diaz, P. M. R. e Silva, V. F. Carvalho, P. J. Jose, R. J. Flower, M. Perretti, and M. A. Martins
A Novel Effect for Annexin 1-Derived Peptide Ac2-26: Reduction of Allergic Inflammation in the Rat
J. Pharmacol. Exp. Ther., June 1, 2005; 313(3): 1416 - 1422.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Maderna, S. Yona, M. Perretti, and C. Godson
Modulation of Phagocytosis of Apoptotic Neutrophils by Supernatant from Dexamethasone-Treated Macrophages and Annexin-Derived Peptide Ac2-26
J. Immunol., March 15, 2005; 174(6): 3727 - 3733.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
C. D. Filippo, R. Marfella, S. Cuzzocrea, E. Piegari, P. Petronella, D. Giugliano, F. Rossi, and M. D'Amico
Hyperglycemia in Streptozotocin-Induced Diabetic Rat Increases Infarct Size Associated With Low Levels of Myocardial HO-1 During Ischemia/Reperfusion
Diabetes, March 1, 2005; 54(3): 803 - 810.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. C. O. Zanardo, M. Perretti, and J. L. Wallace
Annexin-1 is an endogenous gastroprotective factor against indomethacin-induced damage
Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G481 - G486.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. Perretti and R. J. Flower
Annexin 1 and the biology of the neutrophil
J. Leukoc. Biol., July 1, 2004; 76(1): 25 - 29.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Ernst, C. Lange, A. Wilbers, V. Goebeler, V. Gerke, and U. Rescher
An Annexin 1 N-Terminal Peptide Activates Leukocytes by Triggering Different Members of the Formyl Peptide Receptor Family
J. Immunol., June 15, 2004; 172(12): 7669 - 7676.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F. N. E. Gavins, S. Yona, A. M. Kamal, R. J. Flower, and M. Perretti
Leukocyte antiadhesive actions of annexin 1: ALXR- and FPR-related anti-inflammatory mechanisms
Blood, May 15, 2003; 101(10): 4140 - 4147.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
M. Perretti and F. N. E. Gavins
Annexin 1: An Endogenous Anti-Inflammatory Protein
Physiology, April 1, 2003; 18(2): 60 - 64.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Perretti, F. Ingegnoli, S. K. Wheller, M. C. Blades, E. Solito, and C. Pitzalis
Annexin 1 Modulates Monocyte-Endothelial Cell Interaction In Vitro and Cell Migration In Vivo in the Human SCID Mouse Transplantation Model
J. Immunol., August 15, 2002; 169(4): 2085 - 2092.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by The Federation of American Societies for Experimental Biology.