| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,1
* Research Center, Maisonneuve-Rosemont Hospital and Department of Medicine, University of Montréal, Montréal, Québec H1T 2M4 Canada;
Perinatal Research Centre, and
Laboratory of Mass Spectrometry, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2S2 Canada
2Correspondence: Research Center, Maisonneuve-Rosemont Hospital, University of Montréal, 5415 boulevard de l’Assomption, Montréal, Québec H1T 2M4 Canada. E-mail: janos.g.filep{at}umontreal.ca
We recently reported that matrix metalloproteinase 2 (MMP-2, gelatinase A) cleaves big endothelin 1 (ET-1), yielding the vasoactive peptide ET-1[1–32]. We tested whether ET-1[1–32] could affect the adhesion of human neutrophils to coronary artery endothelial cells (HCAEC). ET-1[1–32] rapidly down-regulated the expression of L-selectin and up-regulated expression of CD11b/CD18 on the neutrophil surface, with EC50 values of 1–3 nM. These actions of ET-1[1–32] were mediated via ETA receptors and did not require conversion of ET-1[1–32] into ET-1 by neutrophil proteases, as revealed by liquid chromatography and mass spectroscopy. Moreover, ET-1[1–32] evoked release of neutrophil gelatinase B, which cleaved big ET-1 to yield ET-1[1–32], thus revealing a positive feedback loop for ET-1[1–32] generation. Up-regulation of CD11b/CD18 expression and gelatinase release was tightly associated with activation of extracellular signal-regulated kinase (Erk). Stimulation of Erk activity was due to activation of Ras, Raf-1, and MEK (MAPK kinase). ET-1[1–32] also produced slight increases in the expression of ICAM-1 and E-selectin on HCAEC, and markedly enhanced ß2 integrin-dependent adhesion of neutrophils to activated HCAEC. These results are the first indication that gelatinolytic MMPs via cleavage of big ET-1 to yield ET-1[1–32] activate neutrophils and promote leukocyte-endothelial cell adhesion and, consequently, neutrophil trafficking into inflamed tissues.—Fernandez-Patron, C., Zouki, C., Whittal, R., Chan, J. S. D., Davidge, S. T., Filep, J. G. Matrix metalloproteinases regulate neutrophil-endothelial cell adhesion through generation of endothelin- 1[1–32].
Key Words: gelatinases • ETA receptor • L-selectin • CD11b/CD18 • leukocyte trafficking • endothelium • inflammation
This article has been cited by other articles:
|
|
 |
|
  M. Lin, P. Jackson, A. M. Tester, E. Diaconu, C. M. Overall, J. E. Blalock, and E. Pearlman Matrix Metalloproteinase-8 Facilitates Neutrophil Migration through the Corneal Stromal Matrix by Collagen Degradation and Production of the Chemotactic Peptide Pro-Gly-Pro Am. J. Pathol., July 1, 2008; 173(1): 144 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Reichel, M. Rehberg, P. Bihari, C. M. Moser, S. Linder, A. Khandoga, and F. Krombach Gelatinases mediate neutrophil recruitment in vivo: evidence for stimulus specificity and a critical role in collagen IV remodeling J. Leukoc. Biol., April 1, 2008; 83(4): 864 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Cui, J. Chen, A. Zacharek, Y. Li, C. Roberts, A. Kapke, S. Savant-Bhonsale, and M. Chopp Nitric Oxide Donor Upregulation of Stromal Cell-Derived Factor-1/Chemokine (CXC Motif) Receptor 4 Enhances Bone Marrow Stromal Cell Migration into Ischemic Brain After Stroke Stem Cells, November 1, 2007; 25(11): 2777 - 2785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Greenlee, Z. Werb, and F. Kheradmand Matrix Metalloproteinases in Lung: Multiple, Multifarious, and Multifaceted Physiol Rev, January 1, 2007; 87(1): 69 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Alfonso-Jaume, M. R. Bergman, R. Mahimkar, S. Cheng, Z. Q. Jin, J. S. Karliner, and D. H. Lovett Cardiac ischemia-reperfusion injury induces matrix metalloproteinase-2 expression through the AP-1 components FosB and JunB Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1838 - H1846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Stefanidakis and E. Koivunen Cell-surface association between matrix metalloproteinases and integrins: role of the complexes in leukocyte migration and cancer progression Blood, September 1, 2006; 108(5): 1441 - 1450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Malik, K. Day, I. Perrault, D.S. Charnock-Jones, and S. K. Smith Reduced levels of VEGF-A and MMP-2 and MMP-9 activity and increased TNF-{alpha} in menstrual endometrium and effluent in women with menorrhagia Hum. Reprod., August 1, 2006; 21(8): 2158 - 2166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Khandoga, J. S. Kessler, M. Hanschen, A. G. Khandoga, D. Burggraf, C. Reichel, G. F. Hamann, G. Enders, and F. Krombach Matrix metalloproteinase-9 promotes neutrophil and T cell recruitment and migration in the postischemic liver J. Leukoc. Biol., June 1, 2006; 79(6): 1295 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Tee, J.-L. Vigne, and R. N. Taylor All-Trans Retinoic Acid Inhibits Vascular Endothelial Growth Factor Expression in a Cell Model of Neutrophil Activation Endocrinology, March 1, 2006; 147(3): 1264 - 1270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Souza-Costa, T. Zerbini, A. C. Palei, R. F. Gerlach, and J. E. Tanus-Santos L-arginine Attenuates Acute Pulmonary Embolism-Induced Increases in Lung Matrix Metalloproteinase-2 and Matrix Metalloproteinase-9 Chest, November 1, 2005; 128(5): 3705 - 3710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Thomson, P. Kumarathasan, P. Goegan, R. A. Aubin, and R. Vincent Differential Regulation of the Lung Endothelin System by Urban Particulate Matter and Ozone Toxicol. Sci., November 1, 2005; 88(1): 103 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C.T. Palei, R. A.G. Zaneti, G. M. Fortuna, R. F. Gerlach, and J. E. Tanus-Santos Hemodynamic Benefits of Matrix Metalloproteinase-9 Inhibition by Doxycycline During Experimental Acute Pulmonary Embolism Angiology, September 1, 2005; 56(5): 611 - 617. [Abstract] [PDF]
|
|
|
|
 |
|
 J. S. Ikonomidis, J. R. Barbour, Z. Amani, R. E. Stroud, A. R. Herron, D. M. McClister Jr, S. E. Camens, M. L. Lindsey, R. Mukherjee, and F. G. Spinale Effects of Deletion of the Matrix Metalloproteinase 9 Gene on Development of Murine Thoracic Aortic Aneurysms Circulation, August 30, 2005; 112(9_suppl): I-242 - I-248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Gidday, Y. G. Gasche, J.-C. Copin, A. R. Shah, R. S. Perez, S. D. Shapiro, P. H. Chan, and T. S. Park Leukocyte-derived matrix metalloproteinase-9 mediates blood-brain barrier breakdown and is proinflammatory after transient focal cerebral ischemia Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H558 - H568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. A. Arenas, Y. Xu, P. Lopez-Jaramillo, and S. T. Davidge Angiotensin II-induced MMP-2 release from endothelial cells is mediated by TNF-{alpha} Am J Physiol Cell Physiol, April 1, 2004; 286(4): C779 - C784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.L. Wright, S.G. Farmer, and A. Churg A neutrophil elastase inhibitor reduces cigarette smoke-induced remodelling of lung vessels Eur. Respir. J., July 1, 2003; 22(1): 77 - 81. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
