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Entamoeba histolytica disturbs the tight junction complex in human enteric T84 cell layers

Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, Ghent University Hospital, B-9000 Gent; and
^* Department of Anatomy, Embryology and Histology, Ghent University, B-9000 Gent, Belgium

¹Correspondence: Laboratory of Experimental Cancerology, Department of Radiotherapy and Nuclear Medicine, Ghent University Hospital, De Pintelaan 185, B-9000 Gent, Belgium. E-mail: marc.mareel{at}rug.ac.be

Entamoeba (E.) histolytica trophozoites initiate amebiasis through invasion into the enteric mucosa. It was our aim to understand the molecular interactions between amebic trophozoites and enterocytes during the early steps of invasion. Trophozoites of E. histolytica strain HM1:IMSS were seeded on the apical side of enteric T84 cell layers, which were established on filters in two-compartment culture chambers. Cocultures were analyzed for paracellular permeability by measurement of transepithelial electrical resistance (TER) and for the tight junction proteins ZO-1, ZO-2, occludin, and cingulin by immunocytochemistry and immunoprecipitation. On direct contact with the apical side of the enteric cells, trophozoites caused an increase in paracellular permeability as evidenced by a decrease of TER associated with an increase in [³H]mannitol flux. Immunoprecipitation of cocultures revealed dephosphorylation of ZO-2, loss of ZO-1 from ZO-2, and degradation of ZO-1 but less so of ZO-2 and none of occludin or E-cadherin. In conclusion, trophozoite-associated increase in paracellular permeability of enteric cell layers is ascribed to disturbance of the molecular organization of tight junction proteins.—Leroy, A., Lauwaet, T., De Bruyne, G., Cornelissen, M., Mareel, M. Entamoeba histolytica disturbs the tight junction complex in human enteric T84 cell layers.

Key Words: invasion • transepithelial electrical resistance • paracellular permeability • ZO proteins • amebiasis

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