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Nucleotide excision repair gene (ERCC1) deficiency causes G₂ arrest in hepatocytes and a reduction in liver binucleation: the role of p53 and p21

FATIMA NÚÑEZ^*, MICHAEL D. CHIPCHASE^*, ALAN R. CLARKE and DAVID W. MELTON^*1

^* Institute of Cell and Molecular Biology, Edinburgh University, King’s Buildings, Edinburgh, Scotland, U.K.; and
Department of Pathology, Edinburgh University, Teviot Place, Edinburgh, Scotland, U.K.

¹Correspondence: Institute of Cell and Molecular Biology, Edinburgh University, Darwin Building, King’s Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, U.K. E-mail: David.Melton{at}ed.ac.uk

A wide range of DNA lesions, both UV and chemically induced, are dealt with by the nucleotide excision repair (NER) pathway. Defects in NER result in human syndromes such as xeroderma pigmentosum (XP), where there is a 1000-fold increased incidence of skin cancer. The ERCC1 protein is essential for NER, but ERCC1 knockout mice are not a model for XP. In the absence of exogenous DNA-damaging agents, these mice are runted and die before weaning, with dramatically accelerated liver polyploidy and elevated levels of p53. Here we present a morphological, immunological, and molecular study to understand the mechanism for the unusual liver pathology in ERCC1-deficient mice. We show that the enlarged ERCC1-deficient hepatocytes are arrested in G₂ and that DNA replication and the normal process of binucleation are both reduced. This is associated with a p53-independent increase in expression of the cyclin-dependent kinase inhibitor p21. The most dramatic feature of the ERCC1-deficient liver phenotype, the accelerated polyploidy, is not rescued by p53 deficiency, but we show that p53 is responsible for the reduced DNA replication and binucleation. We consider that the liver phenotype is a response to unrepaired endogenous DNA damage, which may reflect an additional non-NER-related function for the ERCC1 protein.—Núñez, F., Chipchase, M. D., Clarke, A. R., Melton, D. W. Nucleotide excision repair gene (ERCC1) deficiency causes G₂ arrest in hepatocytes and a reduction in liver binucleation: the role of p53 and p21.

Key Words: DNA repair • cancer • knockout mice • cell cycle arrest

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