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(The FASEB Journal. 2000;14:904-912.)
© 2000 FASEB

Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb

MAURIZIO FANCIULLI*1,2, TIZIANA BRUNO*, MONICA DI PADOVA*, ROBERTA DE ANGELIS*,{dagger}, SIMONA IEZZI*, CARLA IACOBINI*, ARISTIDE FLORIDI*,{dagger} and CLAUDIO PASSANANTI{ddagger}1,2

* Cell Metabolism and Pharmacokinetics Laboratory, Regina Elena Cancer Institute, 00158 Rome, Italy;
{dagger} Department of Experimental Medicine, University of L’Aquila, 67100 L’Aquila, Italy; and
{ddagger} Istituto di Tecnologie Biomediche, CNR, 00137 Rome, Italy

1Correspondence: Cell Metabolism and Pharmacokinetics Laboratory, Regina Elena Cancer Institute, Via Delle Messi d’oro 156, 00158 Rome, Italy. E-mail: fanciulli{at}crs.ifo.it; Istituto di Tecnologie Biomediche, CNR, Viale Marx 43, 00137 Rome, Italy. E-mail: passananti{at}itbm.rm.cnr.it

hRPB11 is a core subunit of RNA polymerase II (pol II) specifically down-regulated on doxorubicin (dox) treatment. Levels of this protein profoundly affect cell differentiation, cell proliferation, and tumorigenicity in vivo. Here we describe Che-1, a novel human protein that interacts with hRPB11. Che-1 possesses a domain of high homology with Escherichia coli RNA polymerase {varsigma}-factor 70 and SV40 large T antigen. In addition, we report that Che-1 interacts with the retinoblastoma susceptibility gene (Rb) by two distinct domains. Functionally, we demonstrate that Che-1 represses the growth suppression function of Rb, counteracting the inhibitory action of Rb on the trans-activation function of E2F1. These results identify a novel protein that binds Rb and the core of pol II, and suggest that Che-1 may be part of transcription regulatory complex.—Fanciulli, M., Bruno, T, Di Padova, M., De Angelis, R., Iezzi, S., Iacobini, C, Floridi, A., Passananti, C. Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb.


Key Words: RNA polymerase II • hRPB11 • Che-1 • Retinoblastoma susceptibility gene




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