FASEB J. Thermo Fisher Scientific
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ZAHLER, S.
Right arrow Articles by BECKER, B. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ZAHLER, S.
Right arrow Articles by BECKER, B. F.
(The FASEB Journal. 2000;14:555-564.)
© 2000 FASEB

Endothelial preconditioning by transient oxidative stress reduces inflammatory responses of cultured endothelial cells to TNF-{alpha}

STEFAN ZAHLER1, CHRISTIAN KUPATT and BERNHARD F. BECKER

Department of Physiology, Ludwig-Maximilians-University, Munich, Germany

1Correspondence: Institute of Physiology, Schillerstr. 44, 80336 Munich, Germany. E-mail: s.zahler{at}lrz.uni-muenchen.de

Brief episodes of ischemia can render an organ resistant to subsequent severe ischemia. This ‘ischemic preconditioning’ is ascribed to various mechanisms, including oxidative stress. We investigated whether preconditioning exists on an endothelial level. Human umbilical vein endothelial cells (HUVECs) were transiently confronted with oxidative stress (1 mM H2O2, 5 min). Adhesion molecules ICAM-1 and E-selectin and release of cytokines IL-6 and IL-8 to subsequent stimulation with TNF-{alpha} (2.5 ng/ml, 4 h) were measured (flow cytometry and immunoassay), as were nuclear translocation of the transcription factor NF{kappa}B (Western blotting, confocal microscopy) and redox status of HUVECs (quantification of glutathione by HPLC). TNF-{alpha} elevated IL-6 in the cell supernatant from 8.8 ± 1 to 41 ± 3 pg/ml and IL-8 from 0.5 ± 0.03 to 3 ± 0.2 ng/ml. ICAM-1 was increased threefold and E-selectin rose eightfold. Oxidative stress (decrease of glutathione by 50%) reduced post-TNF-{alpha} levels of IL-6 to 14 ± 3 and IL-8 to 1 ± 0.2; the rise of ICAM-1 was completely blocked and E-selectin was only doubled. The anti-inflammatory effects of preconditioning via oxidative stress were paralleled by reduction of the translocation of NF{kappa}B on stimulation with TNF-{alpha}, and antagonized by the intracellular radical scavenger N-acetylcysteine. ‘Anti-inflammatory preconditioning’ of endothelial cells by oxidative stress may account for the inhibitory effects of preconditioning on leukocyte adhesion in vivo.—Zahler, S., Kupatt, C., Becker, B. F. Endothelial preconditioning by transient oxidative stress reduces inflammatory responses of cultured endothelial cells to TNF-{alpha}.


Key Words: cytokine • adhesion molecule • NF{kappa}B • glutathione




This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
T. P. Obrenovitch
Molecular Physiology of Preconditioning-Induced Brain Tolerance to Ischemia
Physiol Rev, January 1, 2008; 88(1): 211 - 247.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Zhang, T. S. Park, and J. M. Gidday
Hypoxic preconditioning protects human brain endothelium from ischemic apoptosis by Akt-dependent survivin activation
Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H2573 - H2581.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
O. Postea, F. Krotz, A. Henger, C. Keller, and N. Weiss
Stereospecific and Redox-Sensitive Increase in Monocyte Adhesion to Endothelial Cells by Homocysteine
Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 508 - 513.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. A. Zager, A. C. M. Johnson, S. Y. Hanson, and S. Lund
Ischemic proximal tubular injury primes mice to endotoxin-induced TNF-{alpha} generation and systemic release
Am J Physiol Renal Physiol, August 1, 2005; 289(2): F289 - F297.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. C. Schafer, D. N. Sehrt, M. Kamler, H. Jakob, and H.-A. Lehr
Paradoxical attenuation of leukocyte rolling in response to ischemia- reperfusion and extracorporeal blood circulation in inflamed tissue
Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H330 - H335.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
C. Carrasco-Martin, S. Alonso-Orgaz, J. C De la Pinta, M. Marques, C. Macaya, A. Barrientos, M. M Gonzalez, A. Garcia-Mendez, P. J. Mateos-Caceres, J. C Porres, et al.
Endothelial hypoxic preconditioning in rat hypoxic isolated aortic segments
Exp Physiol, July 1, 2005; 90(4): 557 - 569.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Tang, Q. Hao, S. A. Rutherford, B. Low, and Z. J. Zhao
Inactivation of Src Family Tyrosine Kinases by Reactive Oxygen Species in Vivo
J. Biol. Chem., June 24, 2005; 280(25): 23918 - 23925.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
R. Furst, C. Brueckl, W. M. Kuebler, S. Zahler, F. Krotz, A. Gorlach, A. M. Vollmar, and A. K. Kiemer
Atrial Natriuretic Peptide Induces Mitogen-Activated Protein Kinase Phosphatase-1 in Human Endothelial Cells via Rac1 and NAD(P)H Oxidase/Nox2-Activation
Circ. Res., January 7, 2005; 96(1): 43 - 53.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. P. Sorescu, M. Sykes, D. Weiss, M. O. Platt, A. Saha, J. Hwang, N. Boyd, Y. C. Boo, J. D. Vega, W. R. Taylor, et al.
Bone Morphogenic Protein 4 Produced in Endothelial Cells by Oscillatory Shear Stress Stimulates an Inflammatory Response
J. Biol. Chem., August 15, 2003; 278(33): 31128 - 31135.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Z.-Q. Zhao, J. S. Corvera, M. E. Halkos, F. Kerendi, N.-P. Wang, R. A. Guyton, and J. Vinten-Johansen
Inhibition of myocardial injury by ischemic postconditioning during reperfusion: comparison with ischemic preconditioning
Am J Physiol Heart Circ Physiol, August 1, 2003; 285(2): H579 - H588.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
H.-Y. Sohn, F. Krotz, S. Zahler, T. Gloe, M. Keller, K. Theisen, T. M Schiele, V. Klauss, and U. Pohl
Crucial role of local peroxynitrite formation in neutrophil-induced endothelial cell activation
Cardiovasc Res, March 1, 2003; 57(3): 804 - 815.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Z.-Q. Zhao and J. Vinten-Johansen
Myocardial apoptosis and ischemic preconditioning
Cardiovasc Res, August 15, 2002; 55(3): 438 - 455.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Ch. Beck, R. Oberacker, S. Kapper, D. von Zabern, J. Schulte, K. van Ackern, F. J. van der Woude, and B. A. Yard
Modulation of Chemokine Production in Lung Microvascular Endothelial Cells by Dopamine Is Mediated via an Oxidative Mechanism
Am. J. Respir. Cell Mol. Biol., November 1, 2001; 25(5): 636 - 643.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
G. Valen, Z.-q. Yan, and G.o. K. Hansson
Nuclear factor kappa-B and the heart
J. Am. Coll. Cardiol., August 1, 2001; 38(2): 307 - 314.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
I. Jaspers, W. Zhang, A. Fraser, J. M. Samet, and W. Reed
Hydrogen Peroxide Has Opposing Effects on IKK Activity and Ikappa Balpha Breakdown in Airway Epithelial Cells
Am. J. Respir. Cell Mol. Biol., June 1, 2001; 24(6): 769 - 777.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by The Federation of American Societies for Experimental Biology.