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Department of Biology, The University of York, Heslington, York YO10 5YW, United Kingdom
1Correspondence: Bone and Joint Biology Research Group, Department of Biology, The University of York, Heslington, York YO10 5YW, U.K. E-mail: tsg1{at}york.ac.uk
Regulation of bone formation is important in the pathogenesis of many
conditions such as osteoporosis, fracture healing, and loosening of
orthopedic implants. We have recently identified a novel rat cDNA
(best5) by differential display PCR that is regulated
during osteoblast differentiation and bone formation in
vitro and in vivo. Expression of
best5 mRNA is induced in cultures of osteoblasts by both
interferon-
(IFN-) or IFN-
. Whereas IFN- induced a rapid,
transient induction of best5 expression peaking at 4–6
h poststimulation, IFN- elicited a more prolonged induction of
best5 expression, which remained elevated 48 h
poststimulation. A polyclonal antibody generated to a peptide derived
from the best5 coding region recognized a 27 kDa protein
on Western blot analysis of osteoblast lysates. We localized BEST5
protein in osteoblast progenitor cells and mature osteoblasts in
sections of rat tibiae and in sections of bones loaded in
vivo to induce adaptive bone formation. Best5
may therefore be a fundamental intermediate in the response of
osteoblasts to stimuli that modulate proliferation/differentiation,
such as interferons or mechanical loading. These findings highlight the
close interactions between the immune system and bone cells and may
open new therapeutic avenues in modulating bone mass—Grewal, T. S., Genever, P. G., Brabbs, A. C., Birch, M., Skerry, T. M. Best5: a novel interferon-inducible gene expressed
during bone formation.
Key Words: osteoblasts • differentiation • cytokines • osteoporosis • mechanical loading
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