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Radiolabeling revisited: metabolic labeling with ³⁵S-methionine inhibits cell cycle progression, proliferation, and survival

Department of Biochemistry and Molecular Biology, The George Washington University, School of Medicine and Health Sciences, Washington, D.C. 20037, USA

¹Correspondence: Department of Biochemistry and Molecular Biology, The George Washington University, School of Medicine and Health Sciences, Ross Hall, Room 526, 2300 Eye St., N.W., Washington, DC 20037, USA. E-mail: bcmvwh{at}gwumc.edu

Metabolic labeling of cells with low-energy beta-emitting radioisotopes such as [³⁵S]methionine is often used to follow the biosynthesis, maturation, and degradation of proteins in vivo. Such techniques have generally been assumed to be relatively nonperturbing to the cell. The results presented here indicate that metabolic labeling of cells with [³⁵S]methionine under standard experimental conditions can inhibit cell progression into mitosis, cause cell cycle arrest, inhibit cell proliferation in both short-term and colony-forming assays, alter cell morphology, and induce apoptosis over the course of several days. These results thus suggest the need for caution in interpretation of studies using such methods, especially if the experiments rely on the normal progression of the cell cycle or are intended to monitor events occurring in a normally proliferating cell.—Hu, V. W., Heikka, D. S. Radiolabeling revisited: metabolic labeling with ³⁵S-methionine inhibits cell cycle progression, proliferation, and survival.

Key Words: metabolic radiolabeling • beta emitters • cell cycle arrest • apoptosis

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