| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
,¶1
,§,1
* Departments of Nutritional Sciences,
Pathology and Laboratory Medicine,
Medicine, and the
§ Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, Wisconsin 53705, USA;
¶ Pathology and Laboratory Medicine Service and
Geriatric Research, Education and Clinical Center, William S. Middleton VA Hospital, Madison, Wisconsin 53705, USA;
Obesity Research Center, St. Luke’s/Roosevelt Hospital, Columbia University College of Physicians & Surgeons, New York, New York; and
** Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
1Correspondence: T.D.O., Pathology and Laboratory Medicine Service, Room A35, William S. Middleton Memorial Veterans Hospital, 2500 Overlook Terr., Madison, WI 53705, USA. E-mail: toberley{at}facstaff.wisc.edu; R.W., Department of Medicine, William S. Middleton Memorial Veterans Hospital (GRECC 4D), 2500 Overlook Terr., Madison, WI 53705, USA. E-mail: rhweindr{at}facstaff.wisc.edu
In laboratory rodents, caloric restriction (CR) retards several
age-dependent physiological and biochemical changes in skeletal muscle,
including increased steady-state levels of oxidative damage to lipids,
DNA, and proteins. We used immunogold electron microscopic (EM)
techniques with antibodies raised against 4-hydroxy-2-nonenal (HNE)
-modified proteins, dinitrophenol, and nitrotyrosine to quantify and
localize the age-dependent accrual of oxidative damage in rhesus monkey
vastus lateralis skeletal muscle. Using immunogold EM analysis of
muscle from rhesus monkeys ranging in age from 2 to 34 years old, a
fourfold maximal increase in levels of HNE-modified proteins was
observed. Likewise, carbonyl levels increased 
twofold with
aging. Comparing 17- to 23-year-old normally fed to age-matched monkeys
subjected to CR for 10 years, levels of HNE-modified proteins,
carbonyls, and nitrotyrosine in skeletal muscle from the CR group were
significantly less than control group values. Oxidative damage largely
localized to myofibrils, with lesser labeling in other subcellular
compartments. Accumulation of lipid peroxidation-derived aldehydes,
such as malondialdehyde and 4-hydroxy-2-alkenals, and protein carbonyls
were measured biochemically and confirmed the morphological data. Our
study is the first to quantify morphologically and localize the
age-dependent accrual of oxidative damage in mammalian skeletal muscle
and to demonstrate that oxidative damage in primates is lowered by
CR.—Zainal, T. A., Oberley, T. D., Allison, D. B.,
Szweda, L. I., Weindruch, R. Caloric restriction of rhesus monkeys
lowers oxidative damage in skeletal muscle.
Key Words: aging • free radicals • immunogold • lipid peroxidation • reactive oxygen species • sarcopenia
This article has been cited by other articles:
|
|
 |
|
  R. M. Anderson, D. Shanmuganayagam, and R. Weindruch Caloric Restriction and Aging: Studies in Mice and Monkeys Toxicol Pathol, January 1, 2009; 37(1): 47 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-n. Chen, D. A. Ferrington, and L. V. Thompson Carbonic anhydrase III and four-and-a-half LIM protein 1 are preferentially oxidized with muscle unloading J Appl Physiol, November 1, 2008; 105(5): 1554 - 1561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Huffman, D. R. Moellering, W. E. Grizzle, C. R. Stockard, M. S. Johnson, and T. R. Nagy Effect of exercise and calorie restriction on biomarkers of aging in mice Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1618 - R1627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. B Ershler Influence of the Oldest Tumor Host on Tumor Biology Am. Assoc. Cancer Res. Educ. Book, April 12, 2008; 2008(1): 191 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Heydari, A. Unnikrishnan, L. V. Lucente, and A. Richardson Caloric restriction and genomic stability Nucleic Acids Res., December 3, 2007; 35(22): 7485 - 7496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Fontana and S. Klein Aging, Adiposity, and Calorie Restriction JAMA, March 7, 2007; 297(9): 986 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-H. Hyun, S. S. Emerson, D.-G. Jo, M. P. Mattson, and R. de Cabo Calorie restriction up-regulates the plasma membrane redox system in brain cells and suppresses oxidative stress during aging PNAS, December 26, 2006; 103(52): 19908 - 19912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zangarelli, E. Chanseaume, B. Morio, C. Brugere, L. Mosoni, P. Rousset, C. Giraudet, V. Patrac, P. Gachon, Y. Boirie, et al. Synergistic effects of caloric restriction with maintained protein intake on skeletal muscle performance in 21-month-old rats: a mitochondria-mediated pathway FASEB J, December 1, 2006; 20(14): 2439 - 2450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-H. Lee, H. Bartsch, J. Nair, D.-H. Yoo, Y.-C. Hong, S.-H. Cho, and D. Kang Effect of short-term fasting on urinary excretion of primary lipid peroxidation products and on markers of oxidative DNA damage in healthy women Carcinogenesis, July 1, 2006; 27(7): 1398 - 1403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. K. Heilbronn, L. de Jonge, M. I. Frisard, J. P. DeLany, D. E. Larson-Meyer, J. Rood, T. Nguyen, C. K. Martin, J. Volaufova, M. M. Most, et al. Effect of 6-Month Calorie Restriction on Biomarkers of Longevity, Metabolic Adaptation, and Oxidative Stress in Overweight Individuals: A Randomized Controlled Trial JAMA, April 5, 2006; 295(13): 1539 - 1548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Keenan, C.-M. Hoe, L. Mixson, C. L. Mccoy, J. B. Coleman, B. A. Mattson, G. A. Ballam, L. A. Gumprecht, and K. A. Soper Diabesity: A Polygenic Model of Dietary-Induced Obesity from Ad Libitum Overfeeding of Sprague-Dawley Rats and Its Modulation by Moderate and Marked Dietary Restriction Toxicol Pathol, October 1, 2005; 33(6): 650 - 674. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Gredilla and G. Barja Minireview: The Role of Oxidative Stress in Relation to Caloric Restriction and Longevity Endocrinology, September 1, 2005; 146(9): 3713 - 3717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Bevilacqua, J. J. Ramsey, K. Hagopian, R. Weindruch, and M.-E. Harper Long-term caloric restriction increases UCP3 content but decreases proton leak and reactive oxygen species production in rat skeletal muscle mitochondria Am J Physiol Endocrinol Metab, September 1, 2005; 289(3): E429 - E438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Chaiswing, M. P. Cole, W. Ittarat, L. I. Szweda, D. K. St. Clair, and T. D. Oberley Manganese superoxide dismutase and inducible nitric oxide synthase modify early oxidative events in acute Adriamycin-induced mitochondrial toxicity Mol. Cancer Ther., July 1, 2005; 4(7): 1056 - 1064. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. da Costa, C. McGillivray, Q. Bai, J. D. Wood, G. Evans, and K.-C. Chang Restriction of Dietary Energy and Protein Induces Molecular Changes in Young Porcine Skeletal Muscles J. Nutr., September 1, 2004; 134(9): 2191 - 2199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chaiswing, M. P. Cole, D. K. St. Clair, W. Ittarat, L. I. Szweda, and T. D. Oberley Oxidative Damage Precedes Nitrative Damage in Adriamycin-Induced Cardiac Mitochondrial Injury Toxicol Pathol, August 1, 2004; 32(5): 536 - 547. [Abstract] [PDF]
|
|
|
|
|
|
L. Bevilacqua, J. J. Ramsey, K. Hagopian, R. Weindruch, and M.-E. Harper Effects of short- and medium-term calorie restriction on muscle mitochondrial proton leak and reactive oxygen species production Am J Physiol Endocrinol Metab, June 1, 2004; 286(5): E852 - E861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Payne, S. L. Dodd, and C. Leeuwenburgh Life-long calorie restriction in Fischer 344 rats attenuates age-related loss in skeletal muscle-specific force and reduces extracellular space J Appl Physiol, December 1, 2003; 95(6): 2554 - 2562. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. R. Sell, M. A. Lane, M. E. Obrenovich, J. A. Mattison, A. Handy, D. K. Ingram, R. G. Cutler, G. S. Roth, and V. M. Monnier The Effect of Caloric Restriction on Glycation and Glycoxidation in Skin Collagen of Nonhuman Primates J Gerontol A Biol Sci Med Sci, June 1, 2003; 58(6): B508 - B516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Drew, S. Phaneuf, A. Dirks, C. Selman, R. Gredilla, A. Lezza, G. Barja, and C. Leeuwenburgh Effects of aging and caloric restriction on mitochondrial energy production in gastrocnemius muscle and heart Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2003; 284(2): R474 - R480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. S. Roth, M. A. Lane, D. K. Ingram, J. A. Mattison, D. Elahi, J. D. Tobin, D. Muller, and E. J. Metter Biomarkers of Caloric Restriction May Predict Longevity in Humans Science, August 2, 2002; 297(5582): 811 - 811. [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Anderson, K. J. Bitterman, J. G. Wood, O. Medvedik, H. Cohen, S. S. Lin, J. K. Manchester, J. I. Gordon, and D. A. Sinclair Manipulation of a Nuclear NAD+ Salvage Pathway Delays Aging without Altering Steady-state NAD+ Levels J. Biol. Chem., May 17, 2002; 277(21): 18881 - 18890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Droge Free Radicals in the Physiological Control of Cell Function Physiol Rev, January 1, 2002; 82(1): 47 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kayo, D. B. Allison, R. Weindruch, and T. A. Prolla Influences of aging and caloric restriction on the transcriptional profile of skeletal muscle from rhesus monkeys PNAS, April 12, 2001; (2001) 81061898. [Abstract] [Full Text]
|
|
|
|
|
|
R. Weindruch, T. Kayo, C.-K. Lee, and T. A. Prolla Microarray Profiling of Gene Expression in Aging and Its Alteration by Caloric Restriction in Mice J. Nutr., March 1, 2001; 131(3): 918S - 923. [Abstract] [Full Text]
|
|
|
|
|
|
T. Kayo, D. B. Allison, R. Weindruch, and T. A. Prolla Influences of aging and caloric restriction on the transcriptional profile of skeletal muscle from rhesus monkeys PNAS, April 24, 2001; 98(9): 5093 - 5098. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
