| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Institut de Génétique et de Biologie Moléculaire et Cellulaire (CNRS/INSERM/ULP), BP 163, F-67404 ILLKIRCH Cedex, France
1Correspondence: IGBMC, 1 rue Laurent Fries, B.P. 163, 67404 ILLKIRCH, France. E-mail: kedinger{at}igbmc.u-strasbg.fr
Mouse embryonic stem (ES) cells remain pluripotent in vitro when grown in the presence of leukemia inhibitory factor (LIF). LIF withdrawal results in progressive ES cell differentiation. Here we show that during this differentiation process, part of the cells undergo apoptosis concomitant with an activation of the p38 MAP kinase. To gain insight into events mediated by LIF in ES cells, the expression of potential candidate genes was analyzed in the absence or presence of this cytokine by using a semiquantitative RT-PCR assay. We focused on early response genes and on a new type of cytokine repressors (the Socs proteins), some of which exhibit anti-apoptotic properties. We found that expression of c-Fos, c-Jun, and JunB was induced upon LIF treatment whereas that of JunD, the tyrosine phosphatase ESP, and the components of the LIF receptor remained unaffected. Expression of Socs-3, but not Socs-1 or Socs-2, was stimulated in the presence of LIF. Finally, uncontrolled overexpression of Socs-1 and Socs-3 led to repression of LIF-dependent transcription and severely reduced cell viability, suggesting that the disturbance of a well balanced Socs protein content has adverse effects on cell survival.—Duval, D., Reinhardt, B., Kedinger, C., Boeuf, H. Role of suppressors of cytokine signaling (Socs) in leukemia inhibitory factor (LIF) -dependent embryonic stem cell survival.
Key Words: pluripotency • apoptosis • early response genes • transcription
This article has been cited by other articles:
|
|
 |
|
  K. A. Baltgalvis, F. G. Berger, M. M. O. Pena, J. M. Davis, S. J. Muga, and J. A. Carson Interleukin-6 and cachexia in ApcMin/+ mice Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R393 - R401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, H. Xie, X. Sun, S. Tranguch, H. Zhang, X. Jia, D. Wang, S. K. Das, B. Desvergne, W. Wahli, et al. Stage-specific Integration of Maternal and Embryonic Peroxisome Proliferator-activated Receptor {delta} Signaling Is Critical to Pregnancy Success J. Biol. Chem., December 28, 2007; 282(52): 37770 - 37782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Aksoy, C. Sakabedoyan, P.-Y. Bourillot, A. B. Malashicheva, J. Mancip, K. Knoblauch, M. Afanassieff, and P. Savatier Self-Renewal of Murine Embryonic Stem Cells Is Supported by the Serine/Threonine Kinases Pim-1 and Pim-3 Stem Cells, December 1, 2007; 25(12): 2996 - 3004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Magni, E. Dozio, M. Ruscica, H. Watanobe, A. Cariboni, R. Zaninetti, M. Motta, and R. Maggi Leukemia Inhibitory Factor Induces the Chemomigration of Immortalized Gonadotropin-Releasing Hormone Neurons through the Independent Activation of the Janus Kinase/Signal Transducer and Activator of Transcription 3, Mitogen-Activated Protein Kinase/Extracellularly Regulated Kinase 1/2, and Phosphatidylinositol 3-Kinase/Akt Signaling Pathways Mol. Endocrinol., May 1, 2007; 21(5): 1163 - 1174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, S. K. Dey, and M. Maccarrone Jekyll and Hyde: Two Faces of Cannabinoid Signaling in Male and Female Fertility Endocr. Rev., August 1, 2006; 27(5): 427 - 448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bashamboo, A. H. Taylor, K. Samuel, J.-J. Panthier, A. D. Whetton, and L. M. Forrester The survival of differentiating embryonic stem cells is dependent on the SCF-KIT pathway J. Cell Sci., August 1, 2006; 119(15): 3039 - 3046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Emery, H. S. Cate, M. Marriott, T. Merson, M. D. Binder, C. Snell, P. Y. Soo, S. Murray, B. Croker, J.-G. Zhang, et al. Suppressor of cytokine signaling 3 limits protection of leukemia inhibitory factor receptor signaling against central demyelination PNAS, May 16, 2006; 103(20): 7859 - 7864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pritsker, N. R. Ford, H. T. Jenq, and I. R. Lemischka Genomewide gain-of-function genetic screen identifies functionally active genes in mouse embryonic stem cells PNAS, May 2, 2006; 103(18): 6946 - 6951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Aouadi, F. Bost, L. Caron, K. Laurent, Y. Le Marchand Brustel, and B. Binetruy p38 Mitogen-Activated Protein Kinase Activity Commits Embryonic Stem Cells to Either Neurogenesis or Cardiomyogenesis Stem Cells, May 1, 2006; 24(5): 1399 - 1406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Forrai, K. Boyle, A. H. Hart, L. Hartley, S. Rakar, T. A. Willson, K. M. Simpson, A. W. Roberts, W. S. Alexander, A. K. Voss, et al. Absence of Suppressor of Cytokine Signalling 3 Reduces Self-Renewal and Promotes Differentiation in Murine Embryonic Stem Cells Stem Cells, March 1, 2006; 24(3): 604 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Skottman, A.-M. Stromberg, E. Matilainen, J. Inzunza, O. Hovatta, and R. Lahesmaa Unique Gene Expression Signature by Human Embryonic Stem Cells Cultured Under Serum-Free Conditions Correlates with Their Enhanced and Prolonged Growth in an Undifferentiated Stage Stem Cells, January 1, 2006; 24(1): 151 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sekkai, G. Gruel, M. Herry, V. Moucadel, S. N. Constantinescu, O. Albagli, D. T.-L. Roux, W. Vainchenker, and A. Bennaceur-Griscelli Microarray Analysis of LIF/Stat3 Transcriptional Targets in Embryonic Stem Cells Stem Cells, October 1, 2005; 23(10): 1634 - 1642. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J Kimber Leukaemia inhibitory factor in implantation and uterine biology Reproduction, August 1, 2005; 130(2): 131 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Terra, I. Louis, R. Le Blanc, S. Ouellet, J. C. Zuniga-Pflucker, and C. Perreault T-cell generation by lymph node resident progenitor cells Blood, July 1, 2005; 106(1): 193 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Choi, H.-J. Lee, S. Jee, S. Jin, S. K. Koo, S. S. Paik, S. C. Jung, S.-Y. Hwang, K. S. Lee, and B. Oh In Vitro Differentiation of Mouse Embryonic Stem Cells: Enrichment of Endodermal Cells in the Embryoid Body Stem Cells, June 1, 2005; 23(6): 817 - 827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, J. McClintick, L. Zhong, H. J. Edenberg, M. C. Yoder, and R. J. Chan Murine embryonic stem cell differentiation is promoted by SOCS-3 and inhibited by the zinc finger transcription factor Klf4 Blood, January 15, 2005; 105(2): 635 - 637. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gomez-Guerrero, O. Lopez-Franco, G. Sanjuan, P. Hernandez-Vargas, Y. Suzuki, G. Ortiz-Munoz, J. Blanco, and J. Egido Suppressors of Cytokine Signaling Regulate Fc Receptor Signaling and Cell Activation during Immune Renal Injury J. Immunol., June 1, 2004; 172(11): 6969 - 6977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Fedorcsak and R. Storeng Effects of Leptin and Leukemia Inhibitory Factor on Preimplantation Development and STAT3 Signaling of Mouse Embryos In Vitro Biol Reprod, November 1, 2003; 69(5): 1531 - 1538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Kritikou, A. Sharkey, K. Abell, P. J. Came, E. Anderson, R. W. E. Clarkson, and C. J. Watson A dual, non-redundant, role for LIF as a regulator of development and STAT3-mediated cell death in mammary gland Development, August 1, 2003; 130(15): 3459 - 3468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Laszlo and N. M. Nathanson Src Family Kinase-independent Signal Transduction and Gene Induction by Leukemia Inhibitory Factor J. Biol. Chem., July 18, 2003; 278(30): 27750 - 27757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ambrosino, G. Mace, S. Galban, C. Fritsch, K. Vintersten, E. Black, M. Gorospe, and A. R. Nebreda Negative Feedback Regulation of MKK6 mRNA Stability by p38{alpha} Mitogen-Activated Protein Kinase Mol. Cell. Biol., January 1, 2003; 23(1): 370 - 381. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. E. Spangenburg and F. W. Booth Multiple signaling pathways mediate LIF-induced skeletal muscle satellite cell proliferation Am J Physiol Cell Physiol, July 1, 2002; 283(1): C204 - C211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Boeuf, K. Merienne, S. Jacquot, D. Duval, M. Zeniou, C. Hauss, B. Reinhardt, Y. Huss-Garcia, A. Dierich, D. A. Frank, et al. The Ribosomal S6 Kinases, cAMP-responsive Element-binding, and STAT3 Proteins Are Regulated by Different Leukemia Inhibitory Factor Signaling Pathways in Mouse Embryonic Stem Cells J. Biol. Chem., November 30, 2001; 276(49): 46204 - 46211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Paradis and R. Gendron LIF transduces contradictory signals on capillary outgrowth through induction of stat3 and (P41/43)MAP kinase J. Cell Sci., January 12, 2000; 113(23): 4331 - 4339. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
