| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
modulate rapid prolactin release from rat pituitary tumor cells through plasma membrane estrogen receptors
* Department of Human Biological Chemistry and Genetics, and
Department of Pharmacology, University of Texas Medical Branch, Galveston, TX 77555, USA; and
Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
1Correspondence: Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555-0645, USA. E-mail: cswatson{at}utmb.edu
Antibodies (Abs) raised against the estrogen receptor-
(ER) were
used to investigate the role of ER proteins located at the plasma
membrane in mediating the rapid, estrogen-stimulated secretion of
prolactin (PRL) from rat pituitary GH3/B6/F10 cells.
Exposure of the cells to 1 nM 17ß-estradiol (E2)
significantly increased PRL release after 3 or 6 min. When ER Abs
that bind specifically to ER but are too large to diffuse into cells
were tested for activity at the cell membrane, Ab R4, targeted to an
ER hinge region sequence, increased PRL release in a time- and
concentration-dependent fashion. Ab H151, directed against a different
hinge region epitope, decreased PRL release and blocked the stimulatory
action of E2. Abs raised against the DNA binding domain
(H226) or the carboxyl terminus (C542) were not biologically active.
When each Ab was examined for recognition of ER on the cell surface
by immunocytochemistry, all except H151 generated immunostaining in
aldehyde-fixed cells. In live cells, however, Ab H151 but not Ab R4
blocked the membrane binding of fluorescently tagged
E2-BSA. Overall, the data indicate that plasma membrane
ER proteins mediate estrogen-stimulated PRL release from
GH3/B6/F10 cells. These results may also convey information
about conformationally sensitive areas of the membrane form of ER
involved in rapid, nongenomic responses to estrogens.—Norfleet,
A. M., Clarke, C. H., Gametchu, B., Watson, C. S.
Antibodies to the estrogen receptor- modulate rapid prolactin
release from rat pituitary tumor cells through plasma membrane estrogen
receptors.
Key Words: nongenomic effects • membrane steroid receptors • immunocytochemistry
This article has been cited by other articles:
|
|
 |
|
  A. Vasconsuelo, L. Milanesi, and R. Boland 17{beta}-Estradiol abrogates apoptosis in murine skeletal muscle cells through estrogen receptors: role of the phosphatidylinositol 3-kinase/Akt pathway J. Endocrinol., February 1, 2008; 196(2): 385 - 397. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Alonso, M. Moreno, P. Ordonez, R. Fernandez, C. Perez, F. Diaz, A. Navarro, J. Tolivia, and C. Gonzalez Chronic Estradiol Treatment Improves Brain Homeostasis during Aging in Female Rats Endocrinology, January 1, 2008; 149(1): 57 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liu, N. Yanagihara, Y. Toyohira, M. Tsutsui, S. Ueno, and Y. Shinohara Dual Effects of Daidzein, a Soy Isoflavone, on Catecholamine Synthesis and Secretion in Cultured Bovine Adrenal Medullary Cells Endocrinology, November 1, 2007; 148(11): 5348 - 5354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Pietras and D. C. Marquez-Garban Membrane-Associated Estrogen Receptor Signaling Pathways in Human Cancers Clin. Cancer Res., August 15, 2007; 13(16): 4672 - 4676. [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Gilad and B. Schwartz Association of estrogen receptor {beta} with plasma-membrane caveola components: implication in control of vitamin D receptor J. Mol. Endocrinol., June 1, 2007; 38(6): 603 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Tasker, S. Di, and R. Malcher-Lopes Rapid Glucocorticoid Signaling via Membrane-Associated Receptors Endocrinology, December 1, 2006; 147(12): 5549 - 5556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Arreguin-Arevalo and T. M. Nett A Nongenomic Action of 17{beta}-Estradiol as the Mechanism Underlying the Acute Suppression of Secretion of Luteinizing Hormone Biol Reprod, July 1, 2005; 73(1): 115 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. N. Bulayeva, A. L. Wozniak, L. L. Lash, and C. S. Watson Mechanisms of membrane estrogen receptor-{alpha}-mediated rapid stimulation of Ca2+ levels and prolactin release in a pituitary cell line Am J Physiol Endocrinol Metab, February 1, 2005; 288(2): E388 - E397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Du, M. Ohmichi, K. Takahashi, J. Kawagoe, C. Ohshima, H. Igarashi, A. Mori-Abe, M. Saitoh, T. Ohta, A. Ohishi, et al. Both estrogen and raloxifene protect against {beta}-amyloid-induced neurotoxicity in estrogen receptor {alpha}-transfected PC12 cells by activation of telomerase activity via Akt cascade J. Endocrinol., December 1, 2004; 183(3): 605 - 615. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Q. CHEN and J. D. YAGER Estrogen's Effects on Mitochondrial Gene Expression: Mechanisms and Potential Contributions to Estrogen Carcinogenesis Ann. N.Y. Acad. Sci., December 1, 2004; 1028(1): 258 - 272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Q. Chen, M. Delannoy, C. Cooke, and J. D. Yager Mitochondrial localization of ER{alpha} and ER{beta} in human MCF7 cells Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E1011 - E1022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mabuchi, M. Ohmichi, A. Kimura, Y. Nishio, E. Arimoto-Ishida, N. Yada-Hashimoto, K. Tasaka, and Y. Murata Estrogen Inhibits Paclitaxel-Induced Apoptosis via the Phosphorylation of Apoptosis Signal-Regulating Kinase 1 in Human Ovarian Cancer Cell Lines Endocrinology, January 1, 2004; 145(1): 49 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Watson and B. Gametchu Proteins of Multiple Classes May Participate in Nongenomic Steroid Actions Experimental Biology and Medicine, December 1, 2003; 228(11): 1272 - 1281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Braun and P. Thomas Androgens Inhibit Estradiol-17{beta} Synthesis in Atlantic Croaker (Micropogonias undulatus) Ovaries by a Nongenomic Mechanism Initiated at the Cell Surface Biol Reprod, November 1, 2003; 69(5): 1642 - 1650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. LOSEL, E. FALKENSTEIN, M. FEURING, A. SCHULTZ, H.-C. TILLMANN, K. ROSSOL-HASEROTH, and M. WEHLING Nongenomic Steroid Action: Controversies, Questions, and Answers Physiol Rev, July 1, 2003; 83(3): 965 - 1016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Boyan, V. L. Sylvia, T. Frambach, C. H. Lohmann, J. Dietl, D. D. Dean, and Z. Schwartz Estrogen-Dependent Rapid Activation of Protein Kinase C in Estrogen Receptor-Positive MCF-7 Breast Cancer Cells and Estrogen Receptor-Negative HCC38 Cells Is Membrane-Mediated and Inhibited by Tamoxifen Endocrinology, May 1, 2003; 144(5): 1812 - 1824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. H. CAMPBELL, N. BULAYEVA, D. B. BROWN, B. GAMETCHU, and C. S. WATSON Regulation of the membrane estrogen receptor-{alpha}: role of cell density, serum, cell passage number, and estradiol FASEB J, December 1, 2002; 16(14): 1917 - 1927. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Ho and J. K. Liao Nonnuclear Actions of Estrogen Arterioscler. Thromb. Vasc. Biol., December 1, 2002; 22(12): 1952 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Marino, F. Acconcia, F. Bresciani, A. Weisz, and A. Trentalance Distinct Nongenomic Signal Transduction Pathways Controlled by 17beta -Estradiol Regulate DNA Synthesis and Cyclin D1 Gene Transcription in HepG2 Cells Mol. Biol. Cell, October 1, 2002; 13(10): 3720 - 3729. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Erben, D. W. Soegiarto, K. Weber, U. Zeitz, M. Lieberherr, R. Gniadecki, G. Moller, J. Adamski, and R. Balling Deletion of Deoxyribonucleic Acid Binding Domain of the Vitamin D Receptor Abrogates Genomic and Nongenomic Functions of Vitamin D Mol. Endocrinol., July 1, 2002; 16(7): 1524 - 1537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tiffoche, C. Vaillant, D. Schausi, and M.-L. Thieulant Novel Intronic Promoter in the Rat ER{alpha} Gene Responsible for the Transient Transcription of a Variant Receptor Endocrinology, September 1, 2001; 142(9): 4106 - 4119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Vegeto, C. Bonincontro, G. Pollio, A. Sala, S. Viappiani, F. Nardi, A. Brusadelli, B. Viviani, P. Ciana, and A. Maggi Estrogen Prevents the Lipopolysaccharide-Induced Inflammatory Response in Microglia J. Neurosci., March 15, 2001; 21(6): 1809 - 1818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Falkenstein, H.-C. Tillmann, M. Christ, M. Feuring, and M. Wehling Multiple Actions of Steroid Hormones---A Focus on Rapid, Nongenomic Effects Pharmacol. Rev., December 1, 2000; 52(4): 513 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nadal, A. B. Ropero, O. Laribi, M. Maillet, E. Fuentes, and B. Soria Nongenomic actions of estrogens and xenoestrogens by binding at a plasma membrane receptor unrelated to estrogen receptor alpha and estrogen receptor beta PNAS, October 10, 2000; 97(21): 11603 - 11608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Singh, B. D. Schultz, J. A. Katzenellenbogen, E. M. Price, R. J. Bridges, and N. A. Bradbury Estrogen Inhibition of Cystic Fibrosis Transmembrane Conductance Regulator-Mediated Chloride Secretion J. Pharmacol. Exp. Ther., October 1, 2000; 295(1): 195 - 204. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
