| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison. 6153 Medical Sciences Center, Madison, WI 53706, USA
1Correspondence: University of Wisconsin-Madison, Department of Pathology and Laboratory Medicine, 6153 Medical Sciences Center, 1300 University Ave., Madison, WI 53706, USA. E-mail: afriedl{at}facstaff.wisc.edu
Fibroblast growth factors (FGFs) require heparan sulfate proteoglycans (HSPGs) as cofactors for signaling. The heparan sulfate chains (HS) mediate stable high affinity binding of FGFs to their receptor tyrosine kinases (FR) and may specifically regulate FGF activity. A novel in situ binding assay was developed to examine the ability of HSPGs to promote FGF/FR binding using a soluble FR fusion construct (FR1-AP). This fusion protein probe forms a dimer in solution, simulating the dimerization or oligomerization that is thought to occur at the cell surface physiologically. In frozen sections of human skin, FGF-2 binds to keratinocytes and basement membranes of epidermis and dermal blood vessels. In contrast, in skin preincubated with FGF-2, FR1-AP binds avidly to FGF-2 immobilized on keratinocyte cell surfaces, but fails to bind to basement membranes at the dermo-epidermal junction or dermal microvessels despite the fact that these structures bind large amounts of FGF-2. Apparently, basement membrane and cell surface HSPGs differ in their ability to mediate the assembly of a FGF/FR signaling complex presumably due to structural differences of the heparan sulfate chains.—Chang, Z., Meyer, K.,, Rapraeger, A. C., Friedl, A. Differential ability of heparan sulfate proteoglycans to assemble the fibroblast growth factor receptor complex in situ.
Key Words: skin • basement membrane • FGF • cell signaling
This article has been cited by other articles:
|
|
 |
|
  A. Kerever, J. Schnack, D. Vellinga, N. Ichikawa, C. Moon, E. Arikawa-Hirasawa, J. T. Efird, and F. Mercier Novel Extracellular Matrix Structures in the Neural Stem Cell Niche Capture the Neurogenic Factor Fibroblast Growth Factor 2 from the Extracellular Milieu Stem Cells, September 1, 2007; 25(9): 2146 - 2157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. N. Chiu, M.-K. Chung, R. Koistinen, H. Koistinen, M. Seppala, P.-C. Ho, E. H. Y. Ng, K.-F. Lee, and W. S. B. Yeung Cumulus Oophorus-associated Glycodelin-C Displaces Sperm-bound Glycodelin-A and -F and Stimulates Spermatozoa-Zona Pellucida Binding J. Biol. Chem., February 23, 2007; 282(8): 5378 - 5388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Melrose, P. Roughley, S. Knox, S. Smith, M. Lord, and J. Whitelock The Structure, Location, and Function of Perlecan, a Prominent Pericellular Proteoglycan of Fetal, Postnatal, and Mature Hyaline Cartilages J. Biol. Chem., December 1, 2006; 281(48): 36905 - 36914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Dupree, S. R. Pollack, E. M. Levine, and C. T. Laurencin Fibroblast Growth Factor 2 Induced Proliferation in Osteoblasts and Bone Marrow Stromal Cells: A Whole Cell Model Biophys. J., October 15, 2006; 91(8): 3097 - 3112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Jastrebova, M. Vanwildemeersch, A. C. Rapraeger, G. Gimenez-Gallego, U. Lindahl, and D. Spillmann Heparan Sulfate-related Oligosaccharides in Ternary Complex Formation with Fibroblast Growth Factors 1 and 2 and Their Receptors J. Biol. Chem., September 15, 2006; 281(37): 26884 - 26892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Duchesne, B. Tissot, T. R. Rudd, A. Dell, and D. G. Fernig N-Glycosylation of Fibroblast Growth Factor Receptor 1 Regulates Ligand and Heparan Sulfate Co-receptor Binding J. Biol. Chem., September 15, 2006; 281(37): 27178 - 27189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. Culp, L. R. Budgeon, M. P. Marinkovich, G. Meneguzzi, and N. D. Christensen Keratinocyte-secreted laminin 5 can function as a transient receptor for human papillomaviruses by binding virions and transferring them to adjacent cells. J. Virol., September 1, 2006; 80(18): 8940 - 8950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Narita, J. Staub, J. Chien, K. Meyer, M. Bauer, A. Friedl, S. Ramakrishnan, and V. Shridhar HSulf-1 Inhibits Angiogenesis and Tumorigenesis In vivo. Cancer Res., June 15, 2006; 66(12): 6025 - 6032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Dai, Y. Yang, V. MacLeod, X. Yue, A. C. Rapraeger, Z. Shriver, G. Venkataraman, R. Sasisekharan, and R. D. Sanderson HSulf-1 and HSulf-2 Are Potent Inhibitors of Myeloma Tumor Growth in Vivo J. Biol. Chem., December 2, 2005; 280(48): 40066 - 40073. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Pan, M. S. Nelson, M. Reyes, L. Koodie, J. J. Brazil, E. J. Stephenson, R. C. Zhao, C. Peters, S. B. Selleck, S. E. Stringer, et al. Functional abnormalities of heparan sulfate in mucopolysaccharidosis-I are associated with defective biologic activity of FGF-2 on human multipotent progenitor cells Blood, September 15, 2005; 106(6): 1956 - 1964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Whitworth, A. C. Backen, A. R. Clamp, G. Wilson, R. McVey, A. Friedl, A. C. Rapraeger, G. David, A. McGown, R. J. Slade, et al. Regulation of Fibroblast Growth Factor-2 Activity by Human Ovarian Cancer Tumor Endothelium Clin. Cancer Res., June 15, 2005; 11(12): 4282 - 4288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Melrose, S. Smith, and J. Whitelock Perlecan Immunolocalizes to Perichondrial Vessels and Canals in Human Fetal Cartilaginous Primordia in Early Vascular and Matrix Remodeling Events Associated with Diarthrodial Joint Development J. Histochem. Cytochem., November 1, 2004; 52(11): 1405 - 1413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Schmahl, Y. Kim, J. S. Colvin, D. M. Ornitz, and B. Capel Fgf9 induces proliferation and nuclear localization of FGFR2 in Sertoli precursors during male sex determination Development, August 1, 2004; 131(15): 3627 - 3636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. ZCHARIA, S. METZGER, T. CHAJEK-SHAUL, H. AINGORN, M. ELKIN, Y. FRIEDMANN, T. WEINSTEIN, J.-P. LI, U. LINDAHL, and I. VLODAVSKY Transgenic expression of mammalian heparanase uncovers physiological functions of heparan sulfate in tissue morphogenesis, vascularization, and feeding behavior FASEB J, February 1, 2004; 18(2): 252 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Allen and A. C. Rapraeger Spatial and temporal expression of heparan sulfate in mouse development regulates FGF and FGF receptor assembly J. Cell Biol., November 10, 2003; 163(3): 637 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Melrose, S. Smith, P. Ghosh, and J. Whitelock Perlecan, the Multidomain Heparan Sulfate Proteoglycan of Basement Membranes, Is also a Prominent Component of the Cartilaginous Primordia in the Developing Human Fetal Spine J. Histochem. Cytochem., October 1, 2003; 51(10): 1331 - 1341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Goodman, C. S. Branda, M. K. Robinson, R. D. Burdine, and M. J. Stern Alternative splicing affecting a novel domain in the C. elegans EGL-15 FGF receptor confers functional specificity Development, August 15, 2003; 130(16): 3757 - 3766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Qiao, K. Meyer, C. Mundhenke, S. A. Drew, and A. Friedl Heparan Sulfate Proteoglycans as Regulators of Fibroblast Growth Factor-2 Signaling in Brain Endothelial Cells. SPECIFIC ROLE FOR GLYPICAN-1 IN GLIOMA ANGIOGENESIS J. Biol. Chem., April 25, 2003; 278(18): 16045 - 16053. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Raman, G. Venkataraman, S. Ernst, V. Sasisekharan, and R. Sasisekharan Structural specificity of heparin binding in the fibroblast growth factor family of proteins PNAS, March 4, 2003; 100(5): 2357 - 2362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Powell, D. G. Fernig, and J. E. Turnbull Fibroblast Growth Factor Receptors 1 and 2 Interact Differently with Heparin/Heparan Sulfate. IMPLICATIONS FOR DYNAMIC ASSEMBLY OF A TERNARY SIGNALING COMPLEX J. Biol. Chem., August 2, 2002; 277(32): 28554 - 28563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Engbring, M. P. Hoffman, A. J. Karmand, and H. K. Kleinman The B16F10 Cell Receptor for a Metastasis-promoting Site on Laminin-1 Is a Heparan Sulfate/Chondroitin Sulfate-containing Proteoglycan Cancer Res., June 1, 2002; 62(12): 3549 - 3554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-V. Ta, D. Baraniak, J. Julian, J. Korostoff, D.D. Carson, and M.C. Farach-Carson Heparan Sulfate Interacting Protein (HIP/L29) Negatively Regulates Growth Responses to Basic Fibroblast Growth Factor in Gingival Fibroblasts J. Dent. Res., April 1, 2002; 81(4): 247 - 252. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Mundhenke, K. Meyer, S. Drew, and A. Friedl Heparan Sulfate Proteoglycans as Regulators of Fibroblast Growth Factor-2 Receptor Binding in Breast Carcinomas Am. J. Pathol., January 1, 2002; 160(1): 185 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kreuger, M. Salmivirta, L. Sturiale, G. Gimenez-Gallego, and U. Lindahl Sequence Analysis of Heparan Sulfate Epitopes with Graded Affinities for Fibroblast Growth Factors 1 and 2 J. Biol. Chem., August 10, 2001; 276(33): 30744 - 30752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Allen, M. S. Filla, and A. C. Rapraeger Role of heparan sulfate as a tissue-specific regulator of FGF-4 and FGF receptor recognition J. Cell Biol., November 26, 2001; 155(5): 845 - 858. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
