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Research Communications |
Department of Biotechnology and Biosciences, University of Milano-Bicocca, 20126 Milano, Italy; Department of General Physiology and Biochemistry, Laboratory of Electrophysiology, University of Milano, 20133 Milano, Italy;
* Department of Molecular Recognition and Structural Biology, Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca, Morelos 62271, Mexico; and
Institute of General Pathology, University of Florence, I-50134 Firenze, Italy
2Correspondence: Dipartimento di Fisiologia e Biochimica Generali, Via Celoria 26, 20133 Milano, Italy. E-mail: enzo.wanke{at}unimi.it
Toxins isolated from a variety of venoms are tools for probing the physiological function and structure of ion channels. The ether-a-go-go-related genes (erg) codify for the K+ channels (ERG), which are crucial in neurons and are impaired in human long-QT syndrome and Drosophila `seizure' mutants. We have isolated a peptide from the scorpion Centruroides noxius Hoffmann that has no sequence homologies with other toxins, and demonstrate that it specifically inhibits (IC50=16±1 nM) only ERG channels of different species and distinct histogenesis. These results open up the possibility of investigating ERG channel structure–function relationships and novel pharmacological tools with potential therapeutic efficacy.—Gurrola, G. B., Rosati, B., Rocchetti, M., Pimienta, G., Zaza, A., Arcangeli, A., Olivotto, M., Possani, L. D., Wanke, E. A toxin to nervous, cardiac, and endocrine ERG K+ channels isolated from Centruroides noxius scorpion venom.
Key Words: eag gene family • erg gene • ergtoxin • scorpion toxin • action potential
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