| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Research Communications |
dimers stimulate vascular L-type Ca2+ channels via phosphoinositide 3-kinase
a Laboratoire de Physiologie Cellulaire et Pharmacologie Moléculaire, CNRS ESA 5017, Université de Bordeaux II, 33076 Bordeaux, France; and
b Institut für Pharmakologie, Freie Universität Berlin, D-14195 Berlin, Germany
We have previously reported
that, in venous myocytes, Gß
scavengers inhibit angiotensin
AT1A receptor-induced stimulation of L-type
Ca2+ channels (1)
. Here, we demonstrate that intracellular
infusion of purified Gß complexes stimulates the L-type
Ca2+ channel current in a concentration-dependent manner.
Additional intracellular dialysis of GDP-bound inactive
G
o or of a peptide corresponding to the Gß binding
region of the ß-adrenergic receptor kinase completely inhibited the
Gß-induced stimulation of Ca2+ channel currents. The
gating properties of the channel were not affected by intracellular
application of Gß, suggesting that Gß increased the
whole-cell calcium conductance. In addition, both the angiotensin
AT1A receptor- and the Gß-induced stimulation of
L-type Ca2+ channels were blocked by pretreatment of the
cells with wortmannin, at nanomolar concentrations. Correspondingly,
intracellular infusion of an enzymatically active purified recombinant
Gß-sensitive phosphoinositide 3-kinase, PI3K, mimicked
Gß-induced stimulation of Ca2+ channels. Both Gß-
and PI3K-induced stimulations of Ca2+ channel currents
were reduced by protein kinase C inhibitors suggesting that the
Gß/PI3K-activated transduction pathway involves a protein
kinase C. These results indicate for the first time that Gß dimers
stimulate the vascular L-type Ca2+ channels through a
Gß-sensitive PI3K.—Viard, P., Exner, T., Maier, U., Mironneau,
J., Nürnberg, B., Macrez, N. Gß dimers stimulate vascular
L-type Ca2+ channels via phosphoinositide 3-kinase .
Key Words: G-protein • PI3K • protein kinase C • smooth muscle
This article has been cited by other articles:
|
|
 |
|
  Y.-F. Wang and G. I. Hatton Dominant Role of {beta}{gamma} Subunits of G-Proteins in Oxytocin-Evoked Burst Firing J. Neurosci., February 21, 2007; 27(8): 1902 - 1912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Callaghan, J. Zhong, and K. D. Keef Signaling pathway underlying stimulation of L-type Ca2+ channels in rabbit portal vein myocytes by recombinant Gbeta{gamma} subunits Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2541 - H2546. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chansel, M. Ciroldi, S. Vandermeersch, L. F Jackson, A.-M. Gomez, D. Henrion, D. C. Lee, T. M. Coffman, S. Richard, J.-C. Dussaule, et al. Heparin binding EGF is necessary for vasospastic response to endothelin FASEB J, September 1, 2006; 20(11): 1936 - 1938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Eltit, A. A. Garcia, J. Hidalgo, J. L. Liberona, M. Chiong, S. Lavandero, E. Maldonado, and E. Jaimovich Membrane Electrical Activity Elicits Inositol 1,4,5-Trisphosphate-dependent Slow Ca2+ Signals through a Gbeta{gamma}/Phosphatidylinositol 3-Kinase {gamma} Pathway in Skeletal Myotubes J. Biol. Chem., April 28, 2006; 281(17): 12143 - 12154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Vecchione, E. Patrucco, G. Marino, L. Barberis, R. Poulet, A. Aretini, A. Maffei, M. T. Gentile, M. Storto, O. Azzolino, et al. Protection from angiotensin II-mediated vasculotoxic and hypertensive response in mice lacking PI3K{gamma} J. Exp. Med., April 18, 2005; 201(8): 1217 - 1228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.H.S. Arun, C.L. Kaul, and P. Ramarao AT1 receptors and L-type calcium channels: functional coupling in supersensitivity to angiotensin II in diabetic rats Cardiovasc Res, February 1, 2005; 65(2): 374 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Tolloczko, P. Turkewitsch, M. Al-Chalabi, and J. G. Martin LY-294002 [2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one] Affects Calcium Signaling in Airway Smooth Muscle Cells Independently of Phosphoinositide 3-Kinase Inhibition J. Pharmacol. Exp. Ther., November 1, 2004; 311(2): 787 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Le Blanc, C. Mironneau, C. Barbot, M. Henaff, T. Bondeva, R. Wetzker, and N. Macrez Regulation of Vascular L-type Ca2+ Channels by Phosphatidylinositol 3,4,5-Trisphosphate Circ. Res., August 6, 2004; 95(3): 300 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Morel, L. Rakotoarisoa, L. H. Jeyakumar, S. Fleischer, C. Mironneau, and J. Mironneau Decreased Expression of Ryanodine Receptors Alters Calcium-induced Calcium Release Mechanism in mdx Duodenal Myocytes J. Biol. Chem., May 14, 2004; 279(20): 21287 - 21293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Callaghan, S.D. Koh, and K.D. Keef Muscarinic M2 Receptor Stimulation of Cav1.2b Requires Phosphatidylinositol 3-Kinase, Protein Kinase C, and c-Src Circ. Res., March 19, 2004; 94(5): 626 - 633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. McDowell, E. McCall, W. F. Matter, T. B. Estridge, and C. J. Vlahos Phosphoinositide 3-kinase regulates excitation-contraction coupling in neonatal cardiomyocytes Am J Physiol Heart Circ Physiol, February 1, 2004; 286(2): H796 - H805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Northcott and S. W. Watts Low [Mg2+]e Enhances Arterial Spontaneous Tone via Phosphatidylinositol 3-Kinase in DOCA-Salt Hypertension Hypertension, January 1, 2004; 43(1): 125 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Godecke PAF, PIP3 and NO: emerging role in reperfusion injury Cardiovasc Res, November 1, 2003; 60(2): 215 - 216. [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kawanabe, N. Hashimoto, and T. Masaki Effects of nonselective cation channels and PI3K on endothelin-1-induced PYK2 tyrosine phosphorylation in C6 glioma cells Am J Physiol Cell Physiol, September 1, 2003; 285(3): C539 - C545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Morel, N. Fritz, J.-L. Lavie, and J. Mironneau Crucial Role of Type 2 Inositol 1,4,5-Trisphosphate Receptors for Acetylcholine-Induced Ca2+ Oscillations in Vascular Myocytes Arterioscler. Thromb. Vasc. Biol., September 1, 2003; 23(9): 1567 - 1575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kawanabe, N. Hashimoto, and T. Masaki Involvements of Voltage-Independent Ca2+ Channels and Phosphoinositide 3-Kinase in Endothelin-1-Induced PYK2 Tyrosine Phosphorylation Mol. Pharmacol., April 1, 2003; 63(4): 808 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kawanabe, N. Hashimoto, and T. Masaki Role of phosphoinositide 3-kinase in the nonselective cation channel activation by endothelin-1/endothelinB receptor Am J Physiol Cell Physiol, February 1, 2003; 284(2): C506 - C510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Carter and N. L. Kanagy Mechanism of enhanced calcium sensitivity and alpha 2-AR vasoreactivity in chronic NOS inhibition hypertension Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H309 - H316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nassar, S.'e. Akkawi, R. Bar-Shavit, A. Haj-Yehia, K. Bdeir, A.-B. Al-Mehdi, M. Tarshis, and A. A.-R. Higazi Human alpha -defensin regulates smooth muscle cell contraction: a role for low-density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor Blood, December 1, 2002; 100(12): 4026 - 4032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kawanabe, N. Hashimoto, and T. Masaki Effects of Phosphoinositide 3-Kinase on the Endothelin-1-Induced Activation of Voltage-Independent Ca2+ Channels and Mitogenesis in Chinese Hamster Ovary Cells Stably Expressing EndothelinA Receptor Mol. Pharmacol., September 1, 2002; 62(3): 756 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Northcott, M. N. Poy, S. M. Najjar, and S. W. Watts Phosphoinositide 3-Kinase Mediates Enhanced Spontaneous and Agonist-Induced Contraction in Aorta of Deoxycorticosterone Acetate-Salt Hypertensive Rats Circ. Res., August 23, 2002; 91(4): 360 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Poggi, R. Carosio, A. Rubartelli, and M. R. Zocchi {beta}3-Mediated engulfment of apoptotic tumor cells by dendritic cells is dependent on CAMKII: inhibition by HIV-1 Tat J. Leukoc. Biol., March 1, 2002; 71(3): 531 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kimura, K. Sasaki, T. Meguro, and J. H. Zhang Phosphatidylinositol 3-Kinase Inhibitor Failed to Reduce Cerebral Vasospasm in Dog Model of Experimental Subarachnoid Hemorrhage Stroke, February 1, 2002; 33(2): 593 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. D. Keef, J. R. Hume, and J. Zhong Regulation of cardiac and smooth muscle Ca2+ channels (CaV1.2a,b) by protein kinases Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1743 - C1756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. Steinberg PI3King the L-Type Calcium Channel Activation Mechanism Circ. Res., October 12, 2001; 89(8): 641 - 644. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Scholze, T. D. Plant, A. C. Dolphin, and B. Nurnberg Functional Expression and Characterization of a Voltage-Gated CaV1.3 ({{alpha}}1D) Calcium Channel Subunit from an Insulin-Secreting Cell Line Mol. Endocrinol., July 1, 2001; 15(7): 1211 - 1221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Bell, A. J. Butcher, N. S. Berrow, K. M. Page, P. F. Brust, A. Nesterova, K. A. Stauderman, G. R. Seabrook, B. Nurnberg, and A. C. Dolphin Biophysical Properties, Pharmacology, and Modulation of Human, Neuronal L-Type ({alpha}1D, CaV1.3) Voltage-Dependent Calcium Currents J Neurophysiol, February 1, 2001; 85(2): 816 - 827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hernandez-Lopez, T. Tkatch, E. Perez-Garci, E. Galarraga, J. Bargas, H. Hamm, and D. J. Surmeier D2 Dopamine Receptors in Striatal Medium Spiny Neurons Reduce L-Type Ca2+ Currents and Excitability via a Novel PLC{beta}1-IP3-Calcineurin-Signaling Cascade J. Neurosci., December 15, 2000; 20(24): 8987 - 8995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Aoki, A. Y. Zubkov, A. D. Parent, J. H. Zhang, and R. L. Macdonald Mechanism of ATP-Induced [Ca2+]i Mobilization in Rat Basilar Smooth Muscle Cells Editorial Comment Stroke, June 1, 2000; 31(6): 1377 - 1385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Maier, A. Babich, N. Macrez, D. Leopoldt, P. Gierschik, D. Illenberger, and B. Nurnberg Gbeta 5gamma 2 Is a Highly Selective Activator of Phospholipid-dependent Enzymes J. Biol. Chem., April 28, 2000; 275(18): 13746 - 13754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. V. Carman, J.-L. Parent, P. W. Day, A. N. Pronin, P. M. Sternweis, P. B. Wedegaertner, A. G. Gilman, J. L. Benovic, and T. Kozasa Selective Regulation of Galpha q/11 by an RGS Domain in the G Protein-coupled Receptor Kinase, GRK2 J. Biol. Chem., November 26, 1999; 274(48): 34483 - 34492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Maier, A. Babich, and B. Nurnberg Roles of Non-catalytic Subunits in Gbeta gamma -induced Activation of Class I Phosphoinositide 3-Kinase Isoforms beta and gamma J. Biol. Chem., October 8, 1999; 274(41): 29311 - 29317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Meza, R. Bannister, K. Melliti, and B. Adams Biphasic, Opposing Modulation of Cloned Neuronal alpha 1E Ca Channels by Distinct Signaling Pathways Coupled to M2 Muscarinic Acetylcholine Receptors J. Neurosci., August 15, 1999; 19(16): 6806 - 6817. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Kansra, C. Groves, J. C. Gutierrez-Ramos, and R. D. Polakiewicz Phosphatidylinositol 3-Kinase-dependent Extracellular Calcium Influx Is Essential for CX3CR1-mediated Activation of the Mitogen-activated Protein Kinase Cascade J. Biol. Chem., August 17, 2001; 276(34): 31831 - 31838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bacqueville, P. Deleris, C. Mendre, M.-T. Pieraggi, H. Chap, G. Guillon, B. Perret, and M. Breton-Douillon Characterization of a G Protein-activated Phosphoinositide 3-Kinase in Vascular Smooth Muscle Cell Nuclei J. Biol. Chem., June 15, 2001; 276(25): 22170 - 22176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-F. Quignard, J. Mironneau, V. Carricaburu, B. Fournier, A. Babich, B. Nurnberg, C. Mironneau, and N. Macrez Phosphoinositide 3-Kinase gamma Mediates Angiotensin II-induced Stimulation of L-type Calcium Channels in Vascular Myocytes J. Biol. Chem., August 24, 2001; 276(35): 32545 - 32551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Macrez, C. Mironneau, V. Carricaburu, J.-F. Quignard, A. Babich, C. Czupalla, B. Nurnberg, and J. Mironneau Phosphoinositide 3-Kinase Isoforms Selectively Couple Receptors to Vascular L-Type Ca2+ Channels Circ. Res., October 12, 2001; 89(8): 692 - 699. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
