HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by LINSKENS, M. H. K. Articles by HEIKOOP, J. C. Search for Related Content PubMed PubMed Citation Articles by LINSKENS, M. H. K. Articles by HEIKOOP, J. C.

Random mutagenesis and screening of complex glycoproteins: expression of human gonadotropins in Dictyostelium discoideum

^a Cell Engineering Facility GBB, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands; and

^b N.V. Organon, P.O. Box 20, 5340 BH Oss, The Netherlands

The soil amoeba Dictyostelium discoideum is a host cell that provides simple genetics in combination with complex protein synthesis. We show that the complex human heterodimeric gonadotropins can be produced and secreted by this organism. Furthermore, both follicle stimulation hormone and choriogonadotropin produced by D. dictyostelium bind to their human receptors and elicit a biological response comparable to the wild-type hormones. We also show that structure-function analysis using random mutagenesis and screening of recombinant glycoprotein hormones is feasible. Thus, expression of gonadotropins in D. dictyostelium opens the way to the engineering of potential new therapeutic analogues. Linskens, M. H. K., Grootenhuis, P. D. J., Blaauw, M., Huisman-de Winkel, B., van Ravestein, A., van Haastert, P. J. M., and Heikoop, J. C. Random mutagenesis and screening of complex glycoproteins: expression of human gonadotropins in Dictyostelium discoideum.

Key Words: heterologous protein expression • follicle-stimulating hormone • infertility

This article has been cited by other articles:

				R. Yoshino, T. Morio, Y. Yamada, H. Kuwayama, M. Sameshima, Y. Tanaka, H. Sesaki, and M. Iijima Regulation of Ammonia Homeostasis by the Ammonium Transporter AmtA in Dictyostelium discoideum Eukaryot. Cell, December 1, 2007; 6(12): 2419 - 2428. [Abstract] [Full Text] [PDF]

				J. C. Knol, R. Engel, M. Blaauw, A. J. W. G. Visser, and P. J. M. van Haastert The Phosducin-Like Protein PhLP1 Is Essential for G{beta}{gamma} Dimer Formation in Dictyostelium discoideum Mol. Cell. Biol., September 15, 2005; 25(18): 8393 - 8400. [Abstract] [Full Text] [PDF]

				L. Bosgraaf, A. Waijer, R. Engel, A. J. W. G. Visser, D. Wessels, D. Soll, and P. J. M. van Haastert RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium J. Cell Sci., May 1, 2005; 118(9): 1899 - 1910. [Abstract] [Full Text] [PDF]

				D. M. Veltman, J. Roelofs, R. Engel, A. J.W.G. Visser, and P. J.M. Van Haastert Activation of Soluble Guanylyl Cyclase at the Leading Edge during Dictyostelium Chemotaxis Mol. Biol. Cell, February 1, 2005; 16(2): 976 - 983. [Abstract] [Full Text] [PDF]

				E. B. Vervoort, A. v. Ravestein, N. N. M. E. v. Peij, J. C. Heikoop, P. J. M. v. Haastert, G. F. Verheijden, and M. H. K. Linskens Optimizing heterologous expression in Dictyostelium: importance of 5' codon adaptation Nucleic Acids Res., May 15, 2000; 28(10): 2069 - 2074. [Abstract] [Full Text] [PDF]