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Research Communications |
a School of Pharmaceutical Sciences, University of Nottingham;
b Department of Human Anatomy and Cell Biology, Queen's Medical Center, Nottingham; and Laboratories of
c Cell Signaling and Molecular Recognition, The Babraham Institute, Cambridge CB2 4AT, U.K.
Transport of antigens through the follicle-associated epithelium (FAE)
of Peyer's patch (PP) is the critical first step in the induction of
mucosal immune responses. We have previously described that short-term
exposure to Streptococcus pneumoniae R36a induced dramatic
morphological alterations of the FAE in rabbit PP. These results
prompted us to investigate whether the pneumococci-induced
modifications were accompanied by enhanced ability of the FAE to
transport antigens. We addressed this problem by evaluating the ability
of the FAE to bind, internalize, and transport fluorescent polystyrene
microparticles, highly specific to rabbit M cells, after exposure to
S. pneumoniae. Quantitative study revealed a marked
increase in the number of microspheres in PP tissues exposed to
S. pneumoniae compared to tissues exposed to either
phosphate-buffered saline or Escherichia coli DH5
as
controls. No sign of bacterially induced damage to the epithelial
barrier was observed. Further confocal microscopy analysis of the FAE
surface showed that a significant increase in the number of cells that
showed both morphological and functional features of M cells took place
within pneumococci-treated PP tissues. These data provide the first
direct evidence that the FAE-specific antigen sampling function may be
manipulated to improve antigen and drug delivery to the intestinal
immune system.—Meynell, H. M., Thomas, N. W., James, P. S., Holland, J., Taussig, M. J., Nicoletti, C. Up-regulation of
microspheres transport across the follicle-associated epithelium of
Peyer's patch by exposure to Streptococcus pneumoniae
R36a.
Key Words: M cell • enterocyte • mucosal immunity • FAE • antigen delivery
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