| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
* University of California, San Diego, and Cancer Center, La Jolla, California 92093-0652, USA;
Erasmus University, 3000 DR Rotterdam, The Netherlands; and
§ Institut für Klinische Biochemie und Pathobiochemie, Medizinische Universitätsklinik, 97080 Würzburg, Germany
1Correspondence: Department of Medicine, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0652. E-mail: rpilz{at}ucsd.edu
Nitric oxide (NO) and cGMP have been implicated in many neuronal functions, including regulation of gene expression, but little is known about the downstream targets of NO/cGMP in the nervous system. We found that type II cGMP-dependent protein kinase (G-kinase), which is widely expressed in the brain, mediated NO- and cGMP-induced activation of the fos promoter in cells of neuronal and glial origin; the enzyme was ineffective in regulating gene expression in fibroblast-like cells. The effect of G-kinase II on gene expression did not require calcium uptake but was synergistically enhanced by calcium. G-kinase II was membrane associated and did not translocate to the nucleus; however, a soluble G-kinase II mutant translocated to the nucleus and regulated gene expression in fibroblast-like cells. Soluble G-kinase I also regulates fos promoter activity, but membrane targeting of G-kinase I prevented the enzyme from translocating to the nucleus and regulating transcription in multiple cell types, including glioma cells; this suggests that cell type-specific factor(s) that mediate the transcriptional effects of extranuclear G-kinase II are not regulated by G-kinase I. Our results suggest that G-kinase I and II control gene expression by different mechanisms and that NO effects on neuronal plasticity may involve G-kinase II regulation of gene expression.—Gudi, T., Hong, G. K.-P., Vaandrager, A. B., Lohmann, S. M., Pilz, R. B. Nitric oxide and cGMP regulate gene expression in neuronal and glial cells by activating type II cGMP-dependent protein kinase.
Key Words: NO/cGMP signaling • nervous system • fos promoter
This article has been cited by other articles:
|
|
 |
|
  K. E. Broderick, T. Zhang, H. Rangaswami, Y. Zeng, X. Zhao, G. R. Boss, and R. B. Pilz Guanosine 3',5'-Cyclic Monophosphate (cGMP)/cGMP-Dependent Protein Kinase Induce Interleukin-6 Transcription in Osteoblasts Mol. Endocrinol., May 1, 2007; 21(5): 1148 - 1162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jurado, F. Rodriguez-Pascual, J. Sanchez-Prieto, F. M. Reimunde, S. Lamas, and M. Torres NMDA induces post-transcriptional regulation of {alpha}2-guanylyl-cyclase-subunit expression in cerebellar granule cells J. Cell Sci., April 15, 2006; 119(8): 1622 - 1631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhao, S. Zhuang, Y. Chen, G. R. Boss, and R. B. Pilz Cyclic GMP-dependent Protein Kinase Regulates CCAAT Enhancer-binding Protein {beta} Functions through Inhibition of Glycogen Synthase Kinase-3 J. Biol. Chem., September 23, 2005; 280(38): 32683 - 32692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhuang, G. T. Nguyen, Y. Chen, T. Gudi, M. Eigenthaler, T. Jarchau, U. Walter, G. R. Boss, and R. B. Pilz Vasodilator-stimulated Phosphoprotein Activation of Serum-response Element-dependent Transcription Occurs Downstream of RhoA and Is Inhibited by cGMP-dependent Protein Kinase Phosphorylation J. Biol. Chem., March 12, 2004; 279(11): 10397 - 10407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Marcet-Palacios, K. Graham, C. Cass, A. D. Befus, I. Mayers, and M. W. Radomski Nitric Oxide and Cyclic GMP Increase the Expression of Matrix Metalloproteinase-9 in Vascular Smooth Muscle J. Pharmacol. Exp. Ther., October 1, 2003; 307(1): 429 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jurado, J. Sanchez-Prieto, and M. Torres Differential expression of NO-sensitive guanylyl cyclase subunits during the development of rat cerebellar granule cells: regulation via N-methyl-D-aspartate receptors J. Cell Sci., August 1, 2003; 116(15): 3165 - 3175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen, S. Zhuang, S. Cassenaer, D. E. Casteel, T. Gudi, G. R. Boss, and R. B. Pilz Synergism between Calcium and Cyclic GMP in Cyclic AMP Response Element-Dependent Transcriptional Regulation Requires Cooperation between CREB and C/EBP-{beta} Mol. Cell. Biol., June 15, 2003; 23(12): 4066 - 4082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gudi, J. C. Chen, D. E. Casteel, T. M. Seasholtz, G. R. Boss, and R. B. Pilz cGMP-dependent Protein Kinase Inhibits Serum-response Element-dependent Transcription by Inhibiting Rho Activation and Functions J. Biol. Chem., September 27, 2002; 277(40): 37382 - 37393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-F. Lu and R. D. Hawkins Ryanodine Receptors Contribute to cGMP-Induced Late-Phase LTP and CREB Phosphorylation in the Hippocampus J Neurophysiol, September 1, 2002; 88(3): 1270 - 1278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Casteel, S. Zhuang, T. Gudi, J. Tang, M. Vuica, S. Desiderio, and R. B. Pilz cGMP-dependent Protein Kinase Ibeta Physically and Functionally Interacts with the Transcriptional Regulator TFII-I J. Biol. Chem., August 23, 2002; 277(35): 32003 - 32014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Leamey, C. L. Ho-Pao, and M. Sur Disruption of Retinogeniculate Pattern Formation by Inhibition of Soluble Guanylyl Cyclase J. Neurosci., June 1, 2001; 21(11): 3871 - 3880. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
