| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departamento de Estructura y Función de Proteínas, Instituto Reina Sofía de Investigaciones Nefrológicas, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, 28006 Madrid, Spain; and
* Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de Córdoba, 14071 Córdoba, Spain
1Correspondence: Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006, Madrid, Spain. E-mail: pklatt@cib.csic.es (P.K.) or slamas{at}cib.csic.es (S.L.).
Redox control of the transcription factor c-Jun maps to a single cysteine in its DNA binding domain. However, the nature of the oxidized state of this cysteine and, thus, the potential molecular mechanisms accounting for the redox regulation of c-Jun DNA binding remain unclear. To address this issue, we have analyzed the purified recombinant c-Jun DNA binding domain for redox-dependent thiol modifications and concomitant changes in DNA binding activity. We show that changes in the ratio of reduced to oxidized glutathione provide the potential to oxidize c-Jun sulfhydryls by mechanisms that include both protein disulfide formation and S-glutathiolation. We provide evidence that S-glutathiolation, which is specifically targeted to the cysteine residue located in the DNA binding site of the protein, may account for the reversible redox regulation of c-Jun DNA binding. Furthermore, based on a molecular model of the S-glutathiolated protein, we discuss the structural elements facilitating S-glutathiolation and how this modification interferes with DNA binding. Given the structural similarities between the positively charged cysteine-containing DNA binding motif of c-Jun and the DNA binding site of related oxidant-sensitive transcriptional activators, the unprecedented phenomenon of redox-triggered S-thiolation of a transcription factor described in this report suggests a novel role for protein thiolation in the redox control of transcription.—Klatt, P., Molina, E. P., de Lacoba, M. G., Padilla, C. A., Martínez-Galisteo, E., Bárcena, J. A., Lamas, S. Redox regulation of c-Jun DNA binding by reversible S-glutathiolation.
Key Words: glutathione • redox regulation • S • thiolation • transcription factor
This article has been cited by other articles:
|
|
 |
|
  S. W. Aesif, V. Anathy, M. Havermans, A. S. Guala, K. Ckless, D. J. Taatjes, and Y. M.W. Janssen-Heininger In Situ Analysis of Protein S-Glutathionylation in Lung Tissue Using Glutaredoxin-1-Catalyzed Cysteine Derivatization Am. J. Pathol., July 1, 2009; 175(1): 36 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Wilcox, L. Zhou, J. K. Jordon, Y. Huang, Y. Yu, R. L. Redler, X. Chen, M. Caplow, and N. V. Dokholyan Modifications of Superoxide Dismutase (SOD1) in Human Erythrocytes: A POSSIBLE ROLE IN AMYOTROPHIC LATERAL SCLEROSIS J. Biol. Chem., May 15, 2009; 284(20): 13940 - 13947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-J. He, K. E. Mulford, and J. S. Fassler Oxidative Stress Function of the Saccharomyces cerevisiae Skn7 Receiver Domain Eukaryot. Cell, May 1, 2009; 8(5): 768 - 778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lee, Y. J. Lee, H. Choi, E. H. Ko, and J.-w. Kim Reactive Oxygen Species Facilitate Adipocyte Differentiation by Accelerating Mitotic Clonal Expansion J. Biol. Chem., April 17, 2009; 284(16): 10601 - 10609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-H. Gao, M. Bedhomme, D. Veyel, M. Zaffagnini, and S. D. Lemaire Methods for Analysis of Protein Glutathionylation and their Application to Photosynthetic Organisms Mol Plant, March 1, 2009; 2(2): 218 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xie, S. Kole, P. Precht, M. J. Pazin, and M. Bernier S-Glutathionylation Impairs Signal Transducer and Activator of Transcription 3 Activation and Signaling Endocrinology, March 1, 2009; 150(3): 1122 - 1131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Hochgrafe, J. Mostertz, D.-C. Pother, D. Becher, J. D. Helmann, and M. Hecker S-Cysteinylation Is a General Mechanism for Thiol Protection of Bacillus subtilis Proteins after Oxidative Stress J. Biol. Chem., September 7, 2007; 282(36): 25981 - 25985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Koike, T. Hisada, M. Utsugi, T. Ishizuka, Y. Shimizu, A. Ono, Y. Murata, J. Hamuro, M. Mori, and K. Dobashi Glutathione Redox Regulates Airway Hyperresponsiveness and Airway Inflammation in Mice Am. J. Respir. Cell Mol. Biol., September 1, 2007; 37(3): 322 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Martinez-Ruiz and S. Lamas Signalling by NO-induced protein S-nitrosylation and S-glutathionylation: Convergences and divergences Cardiovasc Res, July 15, 2007; 75(2): 220 - 228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Qanungo, D. W. Starke, H. V. Pai, J. J. Mieyal, and A.-L. Nieminen Glutathione Supplementation Potentiates Hypoxic Apoptosis by S-Glutathionylation of p65-NF{kappa}B J. Biol. Chem., June 22, 2007; 282(25): 18427 - 18436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Baker, T. Landowski, R. Dorr, W. R. Tate, J. M.C. Gard, B. E. Tavenner, T. Dragovich, A. Coon, and G. Powis The Antitumor Agent Imexon Activates Antioxidant Gene Expression: Evidence for an Oxidative Stress Response Clin. Cancer Res., June 1, 2007; 13(11): 3388 - 3394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Berndt, C. H. Lillig, and A. Holmgren Thiol-based mechanisms of the thioredoxin and glutaredoxin systems: implications for diseases in the cardiovascular system Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1227 - H1236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Sykes, A. L. Mowbray, and H. Jo Reversible Glutathiolation of Caspase-3 by Glutaredoxin as a Novel Redox Signaling Mechanism in Tumor Necrosis Factor-{alpha}-Induced Cell Death Circ. Res., February 2, 2007; 100(2): 152 - 154. [Full Text] [PDF]
|
|
|
|
 |
|
 N. L. Reynaert, A. van der Vliet, A. S. Guala, T. McGovern, M. Hristova, C. Pantano, N. H. Heintz, J. Heim, Y.-S. Ho, D. E. Matthews, et al. Dynamic redox control of NF-{kappa}B through glutaredoxin-regulated S-glutathionylation of inhibitory {kappa}B kinase beta PNAS, August 29, 2006; 103(35): 13086 - 13091. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Rahman and I. M. Adcock Oxidative stress and redox regulation of lung inflammation in COPD. Eur. Respir. J., July 1, 2006; 28(1): 219 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. C. Chen and O. Ogut Decline of contractility during ischemia-reperfusion injury: actin glutathionylation and its effect on allosteric interaction with tropomyosin Am J Physiol Cell Physiol, March 1, 2006; 290(3): C719 - C727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Cao, F. Kambe, X. Lu, N. Kobayashi, S. Ohmori, and H. Seo Glutathionylation of Two Cysteine Residues in Paired Domain Regulates DNA Binding Activity of Pax-8 J. Biol. Chem., July 8, 2005; 280(27): 25901 - 25906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Xing, M. G. Rosso, and S. Zachgo ROXY1, a member of the plant glutaredoxin family, is required for petal development in Arabidopsis thaliana Development, April 1, 2005; 132(7): 1555 - 1565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. S. Kil and J.-W. Park Regulation of Mitochondrial NADP+-dependent Isocitrate Dehydrogenase Activity by Glutathionylation J. Biol. Chem., March 18, 2005; 280(11): 10846 - 10854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Stocker and J. F. Keaney Jr. Role of Oxidative Modifications in Atherosclerosis Physiol Rev, October 1, 2004; 84(4): 1381 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Lillig, M. E. Lonn, M. Enoksson, A. P. Fernandes, and A. Holmgren Short interfering RNA-mediated silencing of glutaredoxin 2 increases the sensitivity of HeLa cells toward doxorubicin and phenylarsine oxide PNAS, September 7, 2004; 101(36): 13227 - 13232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Forman, J. M. Fukuto, and M. Torres Redox signaling: thiol chemistry defines which reactive oxygen and nitrogen species can act as second messengers Am J Physiol Cell Physiol, August 1, 2004; 287(2): C246 - C256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Conour, W. V. Graham, and H. R. Gaskins A combined in vitro/bioinformatic investigation of redox regulatory mechanisms governing cell cycle progression Physiol Genomics, July 8, 2004; 18(2): 196 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Johansson, C. H. Lillig, and A. Holmgren Human Mitochondrial Glutaredoxin Reduces S-Glutathionylated Proteins with High Affinity Accepting Electrons from Either Glutathione or Thioredoxin Reductase J. Biol. Chem., February 27, 2004; 279(9): 7537 - 7543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Perez-Sala, E. Cernuda-Morollon, and F. J. Canada Molecular Basis for the Direct Inhibition of AP-1 DNA Binding by 15-Deoxy-{Delta}12,14-prostaglandin J2 J. Biol. Chem., December 19, 2003; 278(51): 51251 - 51260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Aracena, G. Sanchez, P. Donoso, S. L. Hamilton, and C. Hidalgo S-Glutathionylation Decreases Mg2+ Inhibition and S-Nitrosylation Enhances Ca2+ Activation of RyR1 Channels J. Biol. Chem., October 31, 2003; 278(44): 42927 - 42935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pastore, G. Tozzi, L. M. Gaeta, E. Bertini, V. Serafini, S. D. Cesare, V. Bonetto, F. Casoni, R. Carrozzo, G. Federici, et al. Actin Glutathionylation Increases in Fibroblasts of Patients with Friedreich's Ataxia: A POTENTIAL ROLE IN THE PATHOGENESIS OF THE DISEASE J. Biol. Chem., October 24, 2003; 278(43): 42588 - 42595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Lillig, A. Potamitou, J.-D. Schwenn, A. Vlamis-Gardikas, and A. Holmgren Redox Regulation of 3'-Phosphoadenylylsulfate Reductase from Escherichia coli by Glutathione and Glutaredoxins J. Biol. Chem., June 13, 2003; 278(25): 22325 - 22330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Borges, T. Geddes, J. T. Watson, and D. M. Kuhn Dopamine Biosynthesis Is Regulated by S-Glutathionylation. POTENTIAL MECHANISM OF TYROSINE HYDROXYLASE INHIBITION DURING OXIDATIVE STRESS J. Biol. Chem., December 6, 2002; 277(50): 48295 - 48302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. A. Comhair and S. C. Erzurum Antioxidant responses to oxidant-mediated lung diseases Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L246 - L255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fuangthong and J. D. Helmann The OhrR repressor senses organic hydroperoxides by reversible formation of a cysteine-sulfenic acid derivative PNAS, May 14, 2002; 99(10): 6690 - 6695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Shenton, G. Perrone, K. A. Quinn, I. W. Dawes, and C. M. Grant Regulation of Protein S-Thiolation by Glutaredoxin 5 in the Yeast Saccharomyces cerevisiae J. Biol. Chem., May 3, 2002; 277(19): 16853 - 16859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fratelli, H. Demol, M. Puype, S. Casagrande, I. Eberini, M. Salmona, V. Bonetto, M. Mengozzi, F. Duffieux, E. Miclet, et al. Identification by redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes PNAS, March 19, 2002; 99(6): 3505 - 3510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Koren, I. Hadari-Naor, E. Zuck, C. Rotem, U. A. Liberman, and A. Ravid Vitamin D Is a Prooxidant in Breast Cancer Cells Cancer Res., February 1, 2001; 61(4): 1439 - 1444. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. A. A. COMHAIR, P. R. BHATHENA, C. FARVER, F. B. J. M. THUNNISSEN, and S. C. ERZURUM Extracellular glutathione peroxidase induction in asthmatic lungs: evidence for redox regulation of expression in human airway epithelial cells FASEB J, January 1, 2001; 15(1): 70 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. J. Thannickal and B. L. Fanburg Reactive oxygen species in cell signaling Am J Physiol Lung Cell Mol Physiol, December 1, 2000; 279(6): L1005 - L1028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. E. MARSHALL, K. MERCHANT, and J. S. STAMLER Nitrosation and oxidation in the regulation of gene expression FASEB J, October 1, 2000; 14(13): 1889 - 1900. [Abstract] [Full Text]
|
|
|
|
|
|
P. Wiesel, L. C. Foster, A. Pellacani, M. D. Layne, C.-M. Hsieh, G. S. Huggins, P. Strauss, S.-F. Yet, and M. A. Perrella Thioredoxin Facilitates the Induction of Heme Oxygenase-1 in Response to Inflammatory Mediators J. Biol. Chem., August 4, 2000; 275(32): 24840 - 24846. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
