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(The FASEB Journal. 1999;13:103-113.)
© 1999 FASEB

Research Communications

Acute hyperglycemia regulates transcription and posttranscriptional stability of PKCßII mRNA in vascular smooth muscle cells

Niketa A. Patela, Charles E. Chalfanta, Mayumi Yamamotoa, James E. Watsonb, Duane C. Eichlera and Denise R. Coopera,b,1

a Departments of Biochemistry and Molecular Biology, College of Medicine, University of South FloridaUSA
b The James A. Haley Veterans Hospital, Tampa, Florida 33612, USA

Acute hyperglycemia may contribute to the progression of atherosclerosis by regulating protein kinase C (PKC) isozymes and by accelerating vascular smooth muscle cell (VSMC) proliferation. We investigated acute glucose regulation of PKCß gene expression in A10 cells, a rat aortic smooth muscle cell line. Western blot analysis showed that PKCßII protein levels decreased with high glucose (25 mM) compared to normal glucose (5.5 mM), whereas PKCßI levels were unaltered. PKCß mRNA levels were depleted by 60–75% in hyperglycemic conditions. To elucidate whether high glucose regulated PKCß expression via the common promoter for PKCßI and PKCßII, deletion constructs of the PKCß promoter ligated to CAT as reporter gene were transfected into A10 cells. Construct D (-411 to +179CAT) showed quenching in high glucose (25 mM) and suggested the involvement of a carbohydrate response element in the 5' promoter region of the PKCß gene. In actinomycin D-treated A10 cells, a 60% decrease in PKCß mRNA with high glucose treatment indicated that posttranscriptional destabilization by glucose was also occurring. We have demonstrated that glucose-induced posttranscriptional destabilization of PKCßII message is mediated via a nuclease activity present in the cytosol. The specificity of glucose to posttranscriptionally destabilize PKCßII mRNA, but not the PKCßI mRNA, was confirmed in both A10 cells and primary cultures from human aorta.—Patel, N. A., Chalfant, C. E., Yamamoto, M., Watson, J. E., Eichler, D. C., Cooper, D. R. Acute hyperglycemia regulates transcription and posttranscriptional stability of PKCßII mRNA in vascular smooth muscle cells. FASEB J. 13, 103–113 (1999)


Key Words: A10 cells • human AoSMC • acute hyperglycemia • posttranscriptional regulation • glucose




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