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RESEARCH COMMUNICATION |
a Institut J. Monod, Université Paris VII-CNRS, 2 place Jussieu, 75251 Paris Cedex 05, France
The yeast plasma membrane, uracil permease, undergoes ubiquitin-dependent endocytosis and subsequent degradation in the vacuole via a process that does not involve the proteasome. Cell-surface ubiquitination of this protein is mediated by the ubiquitin-protein ligase Npi1p/Rsp5p and involves Lys63-linked ubiquitin chains. This report describes the intracellular fate of a mutant form of uracil permease carrying a three amino acid insertion in a cytoplasmic loop. Most of this protein is not deployed beyond the ER, and is degraded by the 26S proteasome. Mutant permease degradation is almost unaffected in cells with impaired Npi1p/Rsp5p, but is dependent on the Ubc6p and Ubc7p ubiquitin-conjugating enzymes, suggesting that proteolysis of the protein requires its prior ubiquitination. Overproduction of a derivative of ubiquitin with a modified Lys48 strongly impairs mutant permease degradation. This suggests that, like other proteasome substrates, mutant permease might be polyubiquitinated with Lys48-linked ubiquitin chains. These findings provide an example of a yeast plasma membrane protein that is routed to the `ER degradation' pathway, and highlight the versatility of the ubiquitin system.—Galan, J.-M., Cantegrit, B., Garnier, C., Namy, O., Haguenauer-Tsapis, R. `ER degradation' of a mutant yeast plasma membrane protein by the ubiquitin-proteasome pathway. FASEB J. 12, 315–323 (1998)
Key Words: proteasome • ubiquitin • uracil permease
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