HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lubec, B. Articles by Lubec, G. Search for Related Content PubMed PubMed Citation Articles by Lubec, B. Articles by Lubec, G.

Aromatic hydroxylation in animal models of diabetes mellitus

^a Department of Neonatology and Intensive Care, University of Vienna, A 1090 Vienna, Austria
^b Institute of Animal Breeding, University of Vienna, A 1090 Vienna, Austria
^c Department of Pediatrics*, University of Vienna, A 1090 Vienna, Austria

Although the involvement of oxidative stress is well documented in the diabetic state, the individual active oxygen species generated have not been demonstrated in animal models of diabetes currently used. Since streptozotocin-induced diabetes mellitus in animals still serves as an animal model of diabetes mellitus, but streptozotocin induces diabetes and generates oxidative stress per se, we decided to study whether aromatic hydroxylation reflecting hydroxyl radical attack was found in three animal models of diabetes mellitus without streptozotocin induction or in streptozotocin-induced diabetes only. For this purpose, we compared lipid peroxidation, aromatic hydroxylation of phenylalanine, glycoxidation in genetically determined diabetic mouse strains db/db and kk, and the diabetic BB rat to these parameters in the streptozotocin-treated rat. Kidney malondialdehyde concentrations, reflecting lipid peroxidation, pentosidine, and N-caboxymethyllysine concentrations, reflecting glycoxidation, were significantly elevated in all diabetic groups as compared to their nondiabetic mates. Aromatic hydroxylation was significantly elevated in the streptozotocin-induced diabetic state exclusively. We conclude that biochemical, pathophysiological, and treatment studies in the streptozotocin model of diabetes mellitus may be confounded by the presence of products, reactions, and tissue damage generated by aromatic hydroxylation reflecting hydroxyl radical attack. We suggest it is not the diabetic state but streptozotocin that generates the hydroxyl radical, as reflected by aromatic hydroxylation in this model.—Lubec, B., Hermon, M., Hoeger, H., Lubec, G. Aromatic hydroxylation in animal models of diabetes mellitus. FASEB J. 12, 1581–1587 (1998)

Key Words: hydroxyl radical • streptozotocin • db/db mouse • BB rat • KK mouse • o-tyrosine • glycoxidation • pentosidine • carboxymethyllysine • malondialdehyde • lipid peroxidation

This article has been cited by other articles:

				M. T. Barati, M. L. Merchant, A. B. Kain, A. W. Jevans, K. R. McLeish, and J. B. Klein Proteomic analysis defines altered cellular redox pathways and advanced glycation end-product metabolism in glomeruli of db/db diabetic mice Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1157 - F1165. [Abstract] [Full Text] [PDF]

				D. Z. Levine, M. Iacovitti, S. J. Robertson, and G. A. Mokhtar Modulation of single-nephron GFR in the db/db mouse model of type 2 diabetes mellitus Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R975 - R981. [Abstract] [Full Text] [PDF]

				M. D. Breyer, E. Bottinger, F. C. Brosius III, T. M. Coffman, R. C. Harris, C. W. Heilig, K. Sharma, and for the AMDCC Mouse Models of Diabetic Nephropathy J. Am. Soc. Nephrol., January 1, 2005; 16(1): 27 - 45. [Abstract] [Full Text] [PDF]

				M.-C. Desco, M. Asensi, R. Marquez, J. Martinez-Valls, M. Vento, F. V. Pallardo, J. Sastre, and J. Vina Xanthine Oxidase Is Involved in Free Radical Production in Type 1 Diabetes: Protection by Allopurinol Diabetes, April 1, 2002; 51(4): 1118 - 1124. [Abstract] [Full Text] [PDF]