Induction of the stress response with prostaglandin A₁ increases I-B gene expression

^a Division of Critical Care Medicine, Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA

I-B is an intracellular protein that functions as a primary inhibitor of the proinflammatory transcription factor NF-B. Induction of the stress response with heat shock was previously demonstrated to induce I-B gene expression. Because the stress response can also be induced by nonthermal stimuli, we determined whether induction of the stress response with prostaglandin A₁ (PGA₁) would induce I-B gene expression. Treatment of human bronchial epithelium (BEAS-2B cells) with PGA₁ induced nuclear translocation of heat shock factor 1, thus confirming that PGA₁ induces the stress response in BEAS-2B cells. Induction of the stress response with PGA₁ increased I-B mRNA expression in a time-dependent manner and increased I-B peptide expression. Transient transfection assays involving a human I-B promoter-luciferase reporter construct demonstrated that induction of the stress response with PGA₁ activated the I-B promoter. Induction of the stress response with PGA₁ and concomitant induction of I-B were associated with inhibition of TNF--mediated secretion of interleukin 8 and with inhibition of TNF--mediated nuclear translocation and activation of NF-B. These data demonstrate that induction of the stress response, by a nonthermal stimulus, increases I-B gene expression by a mechanism involving activation of the I-B promoter. Coupled with previous data demonstrating heat shock-mediated induction of I-B gene expression, these data suggest that I-B may be considered to be one of the stress proteins. The functional consequences of stress response-mediated I-B gene expression may involve attenuation of cellular proinflammatory responses.—Thomas, S. C., Ryan, M. A., Shanley, T. P., Wong, H. R. Induction of the stress response with prostaglandin A₁ increases I-B gene expression. FASEB J. 12, 1371–1378 (1998)

Key Words: PGA₁ • NF-B • interleukin 8 • inflammation

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