| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Fondation pour Recherches Médicales, University of Geneva, 1211 Geneva, Switzerland
b Department of Nutrition, University of Montreal and the CHUM, Centre de Recherche L. C. Simard and Institut du Cancer, Montreal, QC H2L 4M1, Canada
To link glucose signaling to its long-term pleiotropic effects in the pancreatic ß-cell, we have investigated whether glucose regulates immediate-early response genes (IEGs) coding for transcription factors implicated in cell proliferation and differentiation. Glucose causes a coordinated transcriptional activation of the IEGs c-fos, c-jun, JunB, zif-268, and nur-77 in the pancreatic ß-cell line INS-1. This activation is entirely dependent on the presence of the cell-permeant cAMP analog chlorophenylthio-cAMP, which has only a modest effect by itself. The accumulation of c-fos, JunB, and nur-77 mRNA occurs at physiological concentrations of glucose (3 to 11 mM), requires a 1–2 h period, and is mimicked by other nutrient stimuli including mannose, leucine plus glutamine, and pyruvate. Glucose is synergistic with the glucoincretin peptides GLP-1 and PACAP-38, whereas these neurohormonal agents have no effect at low (3 mM) glucose. Mechanistically, the synergy between glucose and the glucoincretins is not based on cAMP alone as glucose does not further increase intracellular cAMP in response to GLP-1 and PACAP-38. A role for Ca2+ signaling is inferred, since the L-type Ca2+ channel blocker nifedipine markedly reduces the induction of c-fos and nur-77 by glucose and GLP-1. The induction of IEGs by glucose and chlorophenylthio-cAMP or GLP-1 and the inhibitory effect of nifedipine are also observed in the ßHC9 cell line. The results indicate that GLP-1 and PACAP-38 act as competence factors for the action of glucose on c-fos, JunB, and nur-77. It is suggested that the synergistic effect of glucose and glucoincretins on IEG expression plays an important role in the adaptive processes of the ß-cell to hyperglycemia.—Susini, S., Roche, E., Prentki, M., Schlegel, W. Glucose and glucoincretin peptides synergize to induce c-fos, c-jun, junB, zif-268, and nur-77 gene expression in pancreatic ß(INS-1) cells. FASEB J. 12, 1173–1182 (1998)
Key Words: glucagon-like peptide-1 • pituitary adenylate cyclase-activating polypeptide • immediate-early response genes • proto-oncogenes • intracellular Ca2+
This article has been cited by other articles:
|
|
 |
|
  J.-H. Kang, S.-Y. Chang, H.-J. Jang, D.-B. Kim, G. R. Ryu, S. H. Ko, I.-K. Jeong, Y.-H. Jo, and M.-J. Kim Exendin-4 inhibits interleukin-1{beta}-induced iNOS expression at the protein level, but not at the transcriptional and posttranscriptional levels, in RINm5F {beta}-cells J. Endocrinol., July 1, 2009; 202(1): 65 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Liu, A. E Dear, L. B Knudsen, and R. W Simpson A long-acting glucagon-like peptide-1 analogue attenuates induction of plasminogen activator inhibitor type-1 and vascular adhesion molecules J. Endocrinol., April 1, 2009; 201(1): 59 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Pearen, S. A. Myers, S. Raichur, J. G. Ryall, G. S. Lynch, and G. E. O. Muscat The Orphan Nuclear Receptor, NOR-1, a Target of {beta}-Adrenergic Signaling, Regulates Gene Expression that Controls Oxidative Metabolism in Skeletal Muscle Endocrinology, June 1, 2008; 149(6): 2853 - 2865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Liu, Y. Hu, R. W Simpson, and A. E Dear Glucagon-like peptide-1 attenuates tumour necrosis factor-{alpha}-mediated induction of plasmogen activator inhibitor-1 expression J. Endocrinol., January 1, 2008; 196(1): 57 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Glauser and W. Schlegel Sequential actions of ERK1/2 on the AP-1 transcription factor allow temporal integration of metabolic signals in pancreatic {beta} cells FASEB J, October 1, 2007; 21(12): 3240 - 3249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Eto, V. Kaur, and M. K. Thomas Regulation of Pancreas Duodenum Homeobox-1 Expression by Early Growth Response-1 J. Biol. Chem., March 2, 2007; 282(9): 5973 - 5983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Kang, M.-J. Kim, H.-I. Jang, K.-H. Koh, K.-S. Yum, D.-J. Rhie, S. H. Yoon, S. J. Hahn, M.-S. Kim, and Y.-H. Jo Proximal cyclic AMP response element is essential for exendin-4 induction of rat EGR-1 gene Am J Physiol Endocrinol Metab, January 1, 2007; 292(1): E215 - E222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Glauser and W. Schlegel Mechanisms of transcriptional regulation underlying temporal integration of signals Nucleic Acids Res., October 6, 2006; (2006) gkl654v3. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ravnskjaer, M. Boergesen, L. T Dalgaard, and S. Mandrup Glucose-induced repression of PPAR{alpha} gene expression in pancreatic {beta}-cells involves PP2A activation and AMPK inactivation. J. Mol. Endocrinol., April 1, 2006; 36(2): 289 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M-J Kim, J-H Kang, Y G Park, G R Ryu, S H Ko, I-K Jeong, K-H Koh, D-J Rhie, S H Yoon, S J Hahn, et al. Exendin-4 induction of cyclin D1 expression in INS-1 {beta}-cells: involvement of cAMP-responsive element. J. Endocrinol., March 1, 2006; 188(3): 623 - 633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Leung-Theung-Long, E. Roulet, P. Clerc, C. Escrieut, S. Marchal-Victorion, B. Ritz-Laser, J. Philippe, L. Pradayrol, C. Seva, D. Fourmy, et al. Essential Interaction of Egr-1 at an Islet-specific Response Element for Basal and Gastrin-dependent Glucagon Gene Transactivation in Pancreatic {alpha}-Cells J. Biol. Chem., March 4, 2005; 280(9): 7976 - 7984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. List and J. F. Habener Glucagon-like peptide 1 agonists and the development and growth of pancreatic {beta}-cells Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E875 - E881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ohsugi, C. Cras-Meneur, Y. Zhou, W. Warren, E. Bernal-Mizrachi, and M. A. Permutt Glucose and Insulin Treatment of Insulinoma Cells Results in Transcriptional Regulation of a Common Set of Genes Diabetes, June 1, 2004; 53(6): 1496 - 1508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Soria, I. Quesada, A. B. Ropero, J. A. Pertusa, F. Martin, and A. Nadal Novel Players in Pancreatic Islet Signaling: From Membrane Receptors to Nuclear Channels Diabetes, February 1, 2004; 53(90001): S86 - 91. [Abstract] [Full Text]
|
|
|
|
|
|
J. A. Ehses, V. R. Casilla, T. Doty, J. A. Pospisilik, K. D. Winter, H.-U. Demuth, R. A. Pederson, and C. H. S. McIntosh Glucose-Dependent Insulinotropic Polypeptide Promotes {beta}-(INS-1) Cell Survival via Cyclic Adenosine Monophosphate-Mediated Caspase-3 Inhibition and Regulation of p38 Mitogen-Activated Protein Kinase Endocrinology, October 1, 2003; 144(10): 4433 - 4445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. R. Merriman-Smith, A. Krushinsky, J. Kistler, and P. J. Donaldson Expression Patterns for Glucose Transporters GLUT1 and GLUT3 in the Normal Rat Lens and in Models of Diabetic Cataract Invest. Ophthalmol. Vis. Sci., August 1, 2003; 44(8): 3458 - 3466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. S. Jhala, G. Canettieri, R. A. Screaton, R. N. Kulkarni, S. Krajewski, J. Reed, J. Walker, X. Lin, M. White, and M. Montminy cAMP promotes pancreatic {beta}-cell survival via CREB-mediated induction of IRS2 Genes & Dev., July 1, 2003; 17(13): 1575 - 1580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Conarello, Z. Li, J. Ronan, R. S. Roy, L. Zhu, G. Jiang, F. Liu, J. Woods, E. Zycband, D. E. Moller, et al. Mice lacking dipeptidyl peptidase IV are protected against obesity and insulin resistance PNAS, May 27, 2003; 100(11): 6825 - 6830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Drucker Glucagon-Like Peptides: Regulators of Cell Proliferation, Differentiation, and Apoptosis Mol. Endocrinol., February 1, 2003; 17(2): 161 - 171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Buteau, S. Foisy, E. Joly, and M. Prentki Glucagon-Like Peptide 1 Induces Pancreatic {beta}-Cell Proliferation Via Transactivation of the Epidermal Growth Factor Receptor Diabetes, January 1, 2003; 52(1): 124 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. E. MacDonald, A. M. F. Salapatek, and M. B. Wheeler Glucagon-Like Peptide-1 Receptor Activation Antagonizes Voltage-Dependent Repolarizing K+ Currents in {beta}-Cells: A Possible Glucose-Dependent Insulinotropic Mechanism Diabetes, December 1, 2002; 51(90003): S443 - 447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-p. Huang, K. Chu, E. Nemoz-Gaillard, D. Elberg, and M.-J. Tsai Neogenesis of {beta}-Cells in Adult BETA2/NeuroD-Deficient Mice Mol. Endocrinol., March 1, 2002; 16(3): 541 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Napolitano, I. Bucci, C. Giuliani, C. Massafra, C. Di Petta, E. Devangelio, D. S. Singer, F. Monaco, and L. D. Kohn High Glucose Levels Increase Major Histocompatibility Complex Class I Gene Expression in Thyroid Cells and Amplify Interferon-{gamma} Action Endocrinology, March 1, 2002; 143(3): 1008 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Lawrence, H. S. Bhatt, and R. A. Easom NFAT Regulates Insulin Gene Promoter Activity in Response to Synergistic Pathways Induced by Glucose and Glucagon-Like Peptide-1 Diabetes, March 1, 2002; 51(3): 691 - 698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bernal-Mizrachi, W. Wen, S. Srinivasan, A. Klenk, D. Cohen, and M. A. Permutt Activation of Elk-1, an Ets transcription factor, by glucose and EGF treatment of insulinoma cells Am J Physiol Endocrinol Metab, December 1, 2001; 281(6): E1286 - E1299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-Q. Ding, E. Holicky, and L. J. Miller Glucose and forskolin regulate IAPP gene expression through different signal transduction pathways Am J Physiol Endocrinol Metab, November 1, 2001; 281(5): E938 - E945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Trumper, K. Trumper, H. Trusheim, R. Arnold, B. Goke, and D. Horsch Glucose-Dependent Insulinotropic Polypeptide Is a Growth Factor for {beta} (INS-1) Cells by Pleiotropic Signaling Mol. Endocrinol., September 1, 2001; 15(9): 1559 - 1570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Song, M. Srinivasan, R. Aalinkeel, and M. S. Patel Use of a cDNA Array for the Identification of Genes Induced in Islets of Suckling Rats by a High-Carbohydrate Nutritional Intervention Diabetes, September 1, 2001; 50(9): 2053 - 2060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Kemp and J. F. Habener Insulinotropic Hormone Glucagon-Like Peptide 1 (GLP-1) Activation of Insulin Gene Promoter Inhibited by p38 Mitogen-Activated Protein Kinase Endocrinology, March 1, 2001; 142(3): 1179 - 1187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Lee and S. G. Laychock Regulation of Inositol Trisphosphate Receptor Isoform Expression in Glucose-Desensitized Rat Pancreatic Islets: Role of Cyclic Adenosine 3',5'-Monophosphate and Calcium Endocrinology, April 1, 2000; 141(4): 1394 - 1402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Mommsen Glucagon-like Peptide-1 in Fishes: The Liver and Beyond Integr. Comp. Biol., April 1, 2000; 40(2): 259 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. SUSINI, G. VAN HAASTEREN, S. LI, M. PRENTKI, and W. SCHLEGEL Essentiality of intron control in the induction of c-fos by glucose and glucoincretin peptides in INS-1 {beta}-cells FASEB J, January 1, 2000; 14(1): 128 - 136. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. J. Kieffer and J. Francis Habener The Glucagon-Like Peptides Endocr. Rev., December 1, 1999; 20(6): 876 - 913. [Abstract] [Full Text]
|
|
|
|
|
|
B. Yusta, R. Somwar, F. Wang, D. Munroe, S. Grinstein, A. Klip, and D. J. Drucker Identification of Glucagon-like Peptide-2 (GLP-2)-activated Signaling Pathways in Baby Hamster Kidney Fibroblasts Expressing the Rat GLP-2 Receptor J. Biol. Chem., October 22, 1999; 274(43): 30459 - 30467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. BERNARD, M.-F. BERTHAULT, C. SAULNIER, and A. KTORZA Neogenesis vs. apoptosis as main components of pancreatic ß cell mass changes in glucose-infused normal and mildly diabetic adult rats FASEB J, July 1, 1999; 13(10): 1195 - 1205. [Abstract] [Full Text]
|
|
|
|
|
|
J.-C. Jonas, A. Sharma, W. Hasenkamp, H. Ilkova, G. Patane, R. Laybutt, S. Bonner-Weir, and G. C. Weir Chronic Hyperglycemia Triggers Loss of Pancreatic beta Cell Differentiation in an Animal Model of Diabetes J. Biol. Chem., May 14, 1999; 274(20): 14112 - 14121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bernal-Mizrachi, B. Wice, H. Inoue, and M. A. Permutt Activation of Serum Response Factor in the Depolarization Induction of Egr-1 Transcription in Pancreatic Islet beta -Cells J. Biol. Chem., August 11, 2000; 275(33): 25681 - 25689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Roduit, J. Morin, F. Masse, L. Segall, E. Roche, C. B. Newgard, F. Assimacopoulos-Jeannet, and M. Prentki Glucose Down-regulates the Expression of the Peroxisome Proliferator-activated Receptor-alpha Gene in the Pancreatic beta -Cell J. Biol. Chem., November 10, 2000; 275(46): 35799 - 35806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fatma, D. P. Singh, T. Shinohara, and L. T. Chylack Jr. Transcriptional Regulation of the Antioxidant Protein 2 Gene, a Thiol-specific Antioxidant, by Lens Epithelium-derived Growth Factor to Protect Cells from Oxidative Stress J. Biol. Chem., December 21, 2001; 276(52): 48899 - 48907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Jonas, D. R. Laybutt, G. M. Steil, N. Trivedi, J. G. Pertusa, M. Van de Casteele, G. C. Weir, and J.-C. Henquin High Glucose Stimulates Early Response Gene c-Myc Expression in Rat Pancreatic beta Cells J. Biol. Chem., September 14, 2001; 276(38): 35375 - 35381. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
