HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mergler, S. Articles by Strauss, O. Search for Related Content PubMed PubMed Citation Articles by Mergler, S. Articles by Strauss, O.

Altered regulation of L-type channels by protein kinase C and protein tyrosine kinases as a pathophysiologic effect in retinal degeneration¹

^a Institut für Klinische Physiologie, Universitätsklinikum Benjamin-Franklin der Freien Universität Berlin, 12200 Berlin, Germany

The effect of protein tyrosine kinases (PTK) on L-type calcium channels in cultured retinal pigmented epithelium (RPE) from rats with retinal dystrophy was investigated. Barium currents through Bay K 8644 (10^-6 M) sensitive L-type channels were measured using the patch-clamp technique. The current density of L-type currents is twice as high and the inactivation time constants are much slower than in cells from nondystrophic control rats. Application of the PTK blockers genistein, lavendustin A, and herbimycin A (all 5x10^-6 M) led to an increase of L-type currents. Intracellular application of pp60c-src (30 U/ml) via the patch pipette led to a transient decrease of L-type currents. The protein kinase A (PKA) and PKG blocker H9 (10^-6 M) showed no effect on L-type currents. However, the protein kinase C blocker chelerythrine (10^-5 M) reduced these currents. Up-regulation of PKC by 10^-6 M 4ß-phorbol-12 myristate-13 acetate (PMA) led to a decrease of L-type currents. Additional application of genistein led to a further decrease of these currents. However, intracellular application of pp60^c-src in PMA-treated cells led to a transient increase of L-type currents. Investigating the calcium response to bFGF application showed that RPE cells from RCS rats used different pathways than control RPE cells to increase cytosolic free calcium. This different pathway does not involve the activation of L-type channels. The present study with RPE cells from rats with retinal dystrophy shows a changed integration of PTK and PKC in channel regulation. Considering the altered response to bFGF in RCS-RPE cells, this disturbed regulation of L-type channels by tyrosine kinases is involved in the etiology of retinal degeneration in RCS rats.—Mergler, S., Steinhausen, K., Wiederholt, M., Strauss, O. Altered regulation of L-type channels by protein kinase C and protein tyrosine kinases as a pathophysiologic effect in retinal degeneration. FASEB J. 12, 1125–1134 (1998)

Key Words: retinal pigment epithelium • bFGF • calcium channels • retinal pigment epithelium • RCS rats

This article has been cited by other articles:

				O. Strauss The Retinal Pigment Epithelium in Visual Function Physiol Rev, July 1, 2005; 85(3): 845 - 881. [Abstract] [Full Text] [PDF]

				X. Jin, N. Morsy, J. Winston, P. J. Pasricha, K. Garrett, and H. I. Akbarali Modulation of TRPV1 by nonreceptor tyrosine kinase, c-Src kinase Am J Physiol Cell Physiol, August 1, 2004; 287(2): C558 - C563. [Abstract] [Full Text] [PDF]

				M. J. Davis, X. Wu, T. R. Nurkiewicz, J. Kawasaki, P. Gui, M. A. Hill, and E. Wilson Regulation of ion channels by protein tyrosine phosphorylation Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H1835 - H1862. [Abstract] [Full Text] [PDF]

				R. ROSENTHAL, H. THIEME, and O. STRAUSS Fibroblast growth factor receptor 2 (FGFR2) in brain neurons and retinal pigment epithelial cells act via stimulation of neuroendocrine L-type channels (Cav1.3) FASEB J, April 1, 2001; 15(6): 970 - 977. [Abstract] [Full Text] [PDF]