| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
RESEARCH COMMUNICATION |
a Department of Neurochemistry and Neurotoxicology, Arrhenius Laboratories, Stockholm University, S-10691 Stockholm, Sweden
b Estonian Biocentre, EE-2400, Tartu, Estonia
c Institute of Biochemistry, Medical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
Transportan is a 27 amino acid-long peptide containing 12 functional amino acids from the amino terminus of the neuropeptide galanin and mastoparan in the carboxyl terminus, connected via a lysine. Transportan is a cell-penetrating peptide as judged by indirect immunofluorescence using N
13-biotinyl-transportan. The internalization of biotinyl-transportan is energy independent and takes place efficiently at 37°, 4°, and 0°C. Cellular uptake of transportan is probably not mediated by endocytosis, since it cannot be blocked by treating the cells with phenylarsine oxide or hyperosmolar sucrose solution and is nonsaturable. The kinetics of internalization was studied with the aid of the 125I-labeled peptide. At 37°C, the maximal intracellular concentration is reached in about 20 min. The internalized transportan is protected from trypsin. The cell-penetrating ability of transportan is not restricted by cell type, but seems to be a general feature of this peptide. In Bowes' melanoma cells, transportan first localizes in the outer membrane and cytoplasmatic membrane structures. This is followed by a redistribution into the nuclear membrane and uptake into the nuclei where transportan concentrates in distinct substructures, probably the nucleoli.—Pooga, M., Hällbrink, M., Zorko, M., Langel, Ü. Cell penetration of transportan FASEB J. 12, 67–77 (1998)
Key Words: peptide synthesis • Bowes' melanoma cells • endocytosis • GTPase activity
This article has been cited by other articles:
|
|
 |
|
  C. I. Lopez, S. A. Belmonte, G. A. De Blas, and L. S. Mayorga Membrane-permeant Rab3A triggers acrosomal exocytosis in living human sperm FASEB J, December 1, 2007; 21(14): 4121 - 4130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Veldhoen, S. D. Laufer, A. Trampe, and T. Restle Cellular delivery of small interfering RNA by a non-covalently attached cell-penetrating peptide: quantitative analysis of uptake and biological effect Nucleic Acids Res., December 2, 2006; 34(22): 6561 - 6573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zielinski, K. Kilk, T. Peritz, T. Kannanayakal, K. Y. Miyashiro, E. Eiriksdottir, J. Jochems, U. Langel, and J. Eberwine In vivo identification of ribonucleoprotein-RNA interactions PNAS, January 31, 2006; 103(5): 1557 - 1562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sato, D. S. Hutchinson, T. Bengtsson, A. Floren, U. Langel, T. Horinouchi, B. A. Evans, and R. J. Summers Functional Domains of the Mouse {beta}3-Adrenoceptor Associated with Differential G Protein Coupling J. Pharmacol. Exp. Ther., December 1, 2005; 315(3): 1354 - 1361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tripathi, B. Chaubey, S. Ganguly, D. Harris, R. A Casale, and V. N. Pandey Anti-HIV-1 activity of anti-TAR polyamide nucleic acid conjugated with various membrane transducing peptides Nucleic Acids Res., August 2, 2005; 33(13): 4345 - 4356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Marinova, V. Vukojevic, S. Surcheva, T. Yakovleva, G. Cebers, N. Pasikova, I. Usynin, L. Hugonin, W. Fang, M. Hallberg, et al. Translocation of Dynorphin Neuropeptides across the Plasma Membrane: A PUTATIVE MECHANISM OF SIGNAL TRANSMISSION J. Biol. Chem., July 15, 2005; 280(28): 26360 - 26370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bauer, U. Bauder-Wuest, W. Mier, U. Haberkorn, and M. Eisenhut 131I-Labeled Peptides as Caspase Substrates for Apoptosis Imaging J. Nucl. Med., June 1, 2005; 46(6): 1066 - 1074. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ziegler and J. Seelig Interaction of the Protein Transduction Domain of HIV-1 TAT with Heparan Sulfate: Binding Mechanism and Thermodynamic Parameters Biophys. J., January 1, 2004; 86(1): 254 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. B. Potocky, A. K. Menon, and S. H. Gellman Cytoplasmic and Nuclear Delivery of a TAT-derived Peptide and a {beta}-Peptide after Endocytic Uptake into HeLa Cells J. Biol. Chem., December 12, 2003; 278(50): 50188 - 50194. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Drin, S. Cottin, E. Blanc, A. R. Rees, and J. Temsamani Studies on the Internalization Mechanism of Cationic Cell-penetrating Peptides J. Biol. Chem., August 15, 2003; 278(33): 31192 - 31201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kaushik, A. Basu, P. Palumbo, R. L. Myers, and V. N. Pandey Anti-TAR Polyamide Nucleotide Analog Conjugated with a Membrane-Permeating Peptide Inhibits Human Immunodeficiency Virus Type 1 Production J. Virol., March 19, 2002; 76(8): 3881 - 3891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Beletskii, Y.-K. Hong, J. Pehrson, M. Egholm, and W. M. Strauss PNA interference mapping demonstrates functional domains in the noncoding RNA Xist PNAS, July 31, 2001; 98(16): 9215 - 9220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Muratovska, R. N. Lightowlers, R. W. Taylor, D. M. Turnbull, R. A. J. Smith, J. A. Wilce, S. W. Martin, and M. P. Murphy Targeting peptide nucleic acid (PNA) oligomers to mitochondria within cells by conjugation to lipophilic cations: implications for mitochondrial DNA replication, expression and disease Nucleic Acids Res., May 1, 2001; 29(9): 1852 - 1863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Bultmann, J. S. Busse, and C. R. Brandt Modified FGF4 Signal Peptide Inhibits Entry of Herpes Simplex Virus Type 1 J. Virol., March 15, 2001; 75(6): 2634 - 2645. [Abstract] [Full Text]
|
|
|
|
|
|
P. A. Wender, D. J. Mitchell, K. Pattabiraman, E. T. Pelkey, L. Steinman, and J. B. Rothbard The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake: Peptoid molecular transporters PNAS, November 21, 2000; 97(24): 13003 - 13008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sei, Q.-e. Yang, D. O'Neill, K. Yoshimura, K. Nagashima, and H. Mitsuya Identification of a Key Target Sequence To Block Human Immunodeficiency Virus Type 1 Replication within the gag-pol Transframe Domain J. Virol., May 15, 2000; 74(10): 4621 - 4633. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. POOGA, M. LINDGREN, M. HALLBRINK, E. BRAKENHIELM, and U. LANGEL Galanin-Based Peptides, Galparan and Transportan, with Receptor-Dependent and Independent Activities Ann. N.Y. Acad. Sci., December 21, 1998; 863(1): 450 - 453. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
