Visual sensitivity and interphotoreceptor retinoid binding protein in the mouse: regulation by vitamin A

^a Medical College of Georgia, Department of Ophthalmology, Augusta, Georgia 30912, USA
^b Saint Louis University, Department of Biology, St. Louis, Missouri 63103, USA
^c University of Missouri School of Medicine, Mason Eye Institute, Columbia, Missouri 65212, USA
^d Medical College of Georgia, Department of Cellular Biology and Anatomy, Augusta, Georgia 30912, USA

Interphotoreceptor retinoid binding protein (IRBP) is a retinoid and fatty acid binding glycoprotein secreted by rod and cone photoreceptors in all vertebrates. IRBP is believed to serve as a carrier for retinoids in the bleaching and regeneration cycle of rhodopsin. IRBP protein has been found to be decreased in vitamin A-deprived rats; it is rapidly recovered after retinol repletion. To understand the mechanism for this recovery, we determined whether vitamin A affects transcription and translation of the IRBP gene. Wild-type and transgenic mice harboring the IRBP promoter-CAT reporter fusion gene were maintained on a retinol-deficient diet supplemented with retinoic acid (-A) or on a control diet (+A) for up to 60 wk postweaning. Some of the -A mice were given retinol repletion for 7 days (-A+A). Electroretinography analysis revealed alterations in waveform and a 2 log unit decrease in b-wave sensitivity in the -A mice over a broad range of stimulus wavelengths. Retinol repletion effected a full recovery. Immunochemistry showed a significant decrease in the immunogold-labeled IRBP between the retinal pigment epithelium and the outer segments of the -A mice compared with +A and -A+A mice. Northern blots showed no differences in the amounts of IRBP or CAT mRNA between these three treatment groups. These results suggest that the regulation of IRBP by retinol is not transcriptional.—Liou, G. I., Matragoon, S., Chen, D.-M., Gao, C.-L., Zhang, L., Fei, Y., Katz, M. L., Stark, W. S. Visual sensitivity and interphotoreceptor retinoid binding protein in the mouse: Regulation by vitamin A. FASEB J. 12, 129–138 (1998)

Key Words: vitamin A (retinol) deficiency and repletion • IRBP • photoreceptor • electroretinography

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