| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The FASEB Journal, Vol 11, 905-915, Copyright © 1997 by The Federation of American Societies for Experimental Biology
RESEARCH COMMUNICATIONS |
M Drab, H Haller, R Bychkov, B Erdmann, C Lindschau, H Haase, I Morano, FC Luft and AM Wobus
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Vascular smooth muscle cell (VSMC) differentiation is important in understanding vascular disease; however, no in vitro model is available. Totipotent mouse embryonic stem (ES) cells were used to establish such a model. To test whether the ES cell-derived smooth muscle cells expressed VSMC-specific properties, the differentiated cells were characterized by 1) morphological analysis, 2) gene expression, 3) immunostaining for VSMC-specific proteins, 4) expression of characteristic VSMC ion channels, and 5) formation of [Ca2+]i transients in response to VSMC-specific agonists. Treatment of embryonic stem cell-derived embryoid bodies with retinoic acid and dibutyryl-cyclic adenosine monophosphate (db-cAMP) induced differentiation of spontaneously contracting cell clusters in 67% of embryoid bodies compared with 10% of untreated controls. The highest differentiation rate was observed when retinoic acid and db-cAMP were applied to the embryoid bodies between days 7 and 11 in combination with frequent changes of culture medium. Other protocols with retinoic acid and db-cAMP, as well as single or combined treatment with VEGF, ECGF, bFGF, aFGF, fibronectin, matrigel, or hypoxia did not influence the differentiation rate. Single-cell RT-PCR and sequencing of the PCR products identified myosin heavy chain (MHC) splice variants distinguishing between gut and VSMC isoforms. RT-PCR with VSMC-specific MHC primers and immunostaining confirmed the presence of VSMC transcripts and MHC protein. Furthermore, VSMC expressing MHC had typical ion channels and responded to specific agonists with an increased [Ca2+]i. Here we present a retinoic acid + db-cAMP-inducible embryonic stem cell model of in vitro vasculogenesis. ES cell-derived cells expressing VSMC-specific MHC and functional VSMC properties may be a suitable system to study mechanisms of VSMC differentiation.
This article has been cited by other articles:
|
|
 |
|
  Y. Shang, T. Yoshida, B. A. Amendt, J. F. Martin, and G. K. Owens Pitx2 is functionally important in the early stages of vascular smooth muscle cell differentiation J. Cell Biol., May 1, 2008; 181(3): 461 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Kolodziejska, M.H. Noyan-Ashraf, A. Nagy, A. Bacon, J. Frampton, H.-B. Xin, M. I. Kotlikoff, and M. Husain c-Myb-Dependent Smooth Muscle Cell Differentiation Circ. Res., March 14, 2008; 102(5): 554 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Facucho-Oliveira, J. Alderson, E. C. Spikings, S. Egginton, and J. C. St. John Mitochondrial DNA replication during differentiation of murine embryonic stem cells J. Cell Sci., November 15, 2007; 120(22): 4025 - 4034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, J. B. Gilner, V. L. Bautch, D.-Z. Wang, B. J. Wainwright, S. L. Kirby, and C. Patterson Wnt2 Coordinates the Commitment of Mesoderm to Hematopoietic, Endothelial, and Cardiac Lineages in Embryoid Bodies J. Biol. Chem., January 5, 2007; 282(1): 782 - 791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Xiao, L. Zeng, Z. Zhang, Y. Hu, and Q. Xu Stem cell-derived Sca-1+ progenitors differentiate into smooth muscle cells, which is mediated by collagen IV-integrin {alpha}1/beta1/{alpha}v and PDGF receptor pathways Am J Physiol Cell Physiol, January 1, 2007; 292(1): C342 - C352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sinha, B. R. Wamhoff, M. H. Hoofnagle, J. Thomas, R. L. Neppl, T. Deering, B. P. Helmke, D. K. Bowles, A. V. Somlyo, and G. K. Owens Assessment of Contractility of Purified Smooth Muscle Cells Derived from Embryonic Stem Cells Stem Cells, July 1, 2006; 24(7): 1678 - 1688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Pouget, R. Gautier, M.-A. Teillet, and T. Jaffredo Somite-derived cells replace ventral aortic hemangioblasts and provide aortic smooth muscle cells of the trunk Development, March 15, 2006; 133(6): 1013 - 1022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Wobus and K. R. Boheler Embryonic Stem Cells: Prospects for Developmental Biology and Cell Therapy Physiol Rev, April 1, 2005; 85(2): 635 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. de Leon, C. Montanez, P. Gomez, S. L. Morales-Lazaro, V. Tapia-Ramirez, V. Valadez-Graham, F. Recillas-Targa, D. Yaffe, U. Nudel, and B. Cisneros Dystrophin Dp71 Expression Is Down-regulated during Myogenesis: ROLE OF Sp1 AND Sp3 ON THE Dp71 PROMOTER ACTIVITY J. Biol. Chem., February 18, 2005; 280(7): 5290 - 5299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sinha, M. H. Hoofnagle, P. A. Kingston, M. E. McCanna, and G. K. Owens Transforming growth factor-{beta}1 signaling contributes to development of smooth muscle cells from embryonic stem cells Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1560 - C1568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Miano Channeling to Myocardin Circ. Res., August 20, 2004; 95(4): 340 - 342. [Full Text] [PDF]
|
|
|
|
|
|
B.R. Wamhoff, D.K. Bowles, O.G. McDonald, S. Sinha, A.P. Somlyo, A.V. Somlyo, and G.K. Owens L-type Voltage-Gated Ca2+ Channels Modulate Expression of Smooth Muscle Differentiation Marker Genes via a Rho Kinase/Myocardin/SRF-Dependent Mechanism Circ. Res., August 20, 2004; 95(4): 406 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Mann, J. L. Ray, E. S. Moon, K. M. Sass, and M. R. Benson Calcineurin initiates smooth muscle differentiation in neural crest stem cells J. Cell Biol., May 24, 2004; 165(4): 483 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. GENG Molecular Mechanisms for Cardiovascular Stem Cell Apoptosis and Growth in the Hearts with Atherosclerotic Coronary Disease and Ischemic Heart Failure Ann. N.Y. Acad. Sci., December 1, 2003; 1010(1): 687 - 697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. SCHENKER Ethical Aspects of Advanced Reproductive Technologies Ann. N.Y. Acad. Sci., November 1, 2003; 997(1): 11 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Vanderlaan, G. Y. Oudit, and P. H. Backx Electrophysiological Profiling of Cardiomyocytes in Embryonic Bodies Derived From Human Embryonic Stem Cells: Therapeutic Implications Circ. Res., July 11, 2003; 93(1): 1 - 3. [Full Text] [PDF]
|
|
|
|
|
|
M. Ruhnke, H. Ungefroren, G. Zehle, M. Bader, B. Kremer, and F. Fandrich Long-Term Culture and Differentiation of Rat Embryonic Stem Cell-Like Cells into Neuronal, Glial, Endothelial, and Hepatic Lineages Stem Cells, July 1, 2003; 21(4): 428 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ooto, M. Haruta, Y. Honda, H. Kawasaki, Y. Sasai, and M. Takahashi Induction of the Differentiation of Lentoids from Primate Embryonic Stem Cells Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2689 - 2693. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Kumar and G. K. Owens Combinatorial Control of Smooth Muscle-Specific Gene Expression Arterioscler. Thromb. Vasc. Biol., May 1, 2003; 23(5): 737 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gepstein Derivation and Potential Applications of Human Embryonic Stem Cells Circ. Res., November 15, 2002; 91(10): 866 - 876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shiroi, M. Yoshikawa, H. Yokota, H. Fukui, S. Ishizaka, K. Tatsumi, and Y. Takahashi Identification of Insulin-Producing Cells Derived from Embryonic Stem Cells by Zinc-Chelating Dithizone Stem Cells, July 1, 2002; 20(4): 284 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamada, M. Yoshikawa, S. Kanda, Y. Kato, Y. Nakajima, S. Ishizaka, and Y. Tsunoda In Vitro Differentiation of Embryonic Stem Cells into Hepatocyte-Like Cells Identified by Cellular Uptake of Indocyanine Green Stem Cells, March 1, 2002; 20(2): 146 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamada, M. Yoshikawa, M. Takaki, S. Torihashi, Y. Kato, Y. Nakajima, S. Ishizaka, and Y. Tsunoda In Vitro Functional Gut-Like Organ Formation from Mouse Embryonic Stem Cells Stem Cells, January 1, 2002; 20(1): 41 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. MÜLLER, B. K. FLEISCHMANN, S. SELBERT, G. J. JI, E. ENDL, G. MIDDELER, O. J. MÜLLER, P. SCHLENKE, S. FRESE, A. M. WOBUS, et al. Selection of ventricular-like cardiomyocytes from ES cells in vitro FASEB J, December 1, 2000; 14(15): 2540 - 2548. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Gualandris, J. P. Annes, M. Arese, I. Noguera, V. Jurukovski, and D. B. Rifkin The Latent Transforming Growth Factor-beta -binding Protein-1 Promotes In Vitro Differentiation of Embryonic Stem Cells into Endothelium Mol. Biol. Cell, December 1, 2000; 11(12): 4295 - 4308. [Abstract] [Full Text]
|
|
|
|
|
|
H. Ou, J. Haendeler, M. R. Aebly, L. A. Kelly, B. C. Cholewa, G. Koike, A. Kwitek-Black, H. J. Jacob, B. C. Berk, and J. M. Miano Retinoic Acid-Induced Tissue Transglutaminase and Apoptosis in Vascular Smooth Muscle Cells Circ. Res., November 10, 2000; 87(10): 881 - 887. [Abstract] [Full Text]
|
|
|
|
|
|
J. M. Miano and B. C. Berk Retinoids : Versatile Biological Response Modifiers of Vascular Smooth Muscle Phenotype Circ. Res., September 1, 2000; 87(5): 355 - 362. [Full Text]
|
|
|
|
|
|
P. Komalavilas, P. K. Shah, H. Jo, and T. M. Lincoln Activation of Mitogen-activated Protein Kinase Pathways by Cyclic GMP and Cyclic GMP-dependent Protein Kinase in Contractile Vascular Smooth Muscle Cells J. Biol. Chem., November 26, 1999; 274(48): 34301 - 34309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Neuville, Z.-q Yan, A. Gidlof, M. S. Pepper, G. K. Hansson, G. Gabbiani, and A. Sirsjo Retinoic Acid Regulates Arterial Smooth Muscle Cell Proliferation and Phenotypic Features In Vivo and In Vitro Through an RAR{alpha}-Dependent Signaling Pathway Arterioscler. Thromb. Vasc. Biol., June 1, 1999; 19(6): 1430 - 1436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Xu, G Liguori, M. Persico, and E. Adamson Abrogation of the Cripto gene in mouse leads to failure of postgastrulation morphogenesis and lack of differentiation of cardiomyocytes Development, January 2, 1999; 126(3): 483 - 494. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
