FASEB J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by von Knethen, A.
Right arrow Articles by Brune, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by von Knethen, A.
Right arrow Articles by Brune, B.

The FASEB Journal, Vol 11, 887-895, Copyright © 1997 by The Federation of American Societies for Experimental Biology

RESEARCH COMMUNICATIONS

Cyclooxygenase-2: an essential regulator of NO-mediated apoptosis

A von Knethen and B Brune
University of Erlangen-Nurnberg, Faculty of Medicine, Department of Medicine IV-Experimental Division, Erlangen, Germany.

Lipopolysaccharide/interferon gamma up-regulated inducible nitric oxide synthase and caused nitric oxide generation and concomitant apoptotic cell death in RAW 264.7 macrophages. Exogenously supplied nitric oxide donors such as S-nitrosoglutathione produced equivalent alterations. Preactivation of macrophages with a combination of lipopolysaccharide/interferon gamma under conditions of blocked NO synthase--N(G)-monomethyl-L-arginine addition--or stimulation with a low, nondestructive dose of S-nitrosoglutathione conferred protection against high and thus apoptotic NO concentrations. Here we report that induction of cyclooxygenase-2 during the preactivation period is a critical regulator of macrophage apoptosis. Under resting conditions, macrophages do not express cyclooxygenase-2, whereas lipopolysaccharide/interferon gamma/N(G)-monomethyl-L-arginine prestimulation for 12-15 h caused protein expression. In parallel, preactivation of RAW cells with a low, nontoxic dose of S- nitrosoglutathione promoted protection and cyclooxygenase-2 up- regulation. To prove cyclooxygenase-2 involvement during protection, we stably transfected RAW 264.7 macrophages with a rat cyclooxygenase-2 expression vector. Cyclooxygenase-2 overexpressing macrophages, preactivated with the calcium liberating and thus phopholipase A2- activating agent A23187, revealed protection against exogenously supplied NO. Protection afforded by lipopolysaccharide/interferon gamma/N(G)-monomethyl-L-arginine prestimulation was completely reversed by the addition of the cyclooxygenase-2 selective inhibitor NS-398 or in macrophages stably transfected with an antisense cyclooxygenase-2 expression vector. Our results point to cyclooxygenase-2 induction by lipopolysaccharide/interferon gamma/N(G)-monomethyl-L-arginine or low- dose nitric oxide pretreatment conferring macrophage protection to the apoptotic action of nitric oxide.


This article has been cited by other articles:


Home page
 Circ. Res.Home page
V. Rudolph and B. A. Freeman
Cardiovascular Consequences When Nitric Oxide and Lipid Signaling Converge
Circ. Res., September 11, 2009; 105(6): 511 - 522.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. M. Ndengele, S. Cuzzocrea, E. Esposito, E. Mazzon, R. Di Paola, G. M. Matuschak, and D. Salvemini
Cyclooxygenases 1 and 2 contribute to peroxynitrite-mediated inflammatory pain hypersensitivity
FASEB J, September 1, 2008; 22(9): 3154 - 3164.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Huh, A. Liepins, J. Zielonka, C. Andrekopoulos, B. Kalyanaraman, and A. Sorokin
Cyclooxygenase 2 Rescues LNCaP Prostate Cancer Cells from Sanguinarine-Induced Apoptosis by a Mechanism Involving Inhibition of Nitric Oxide Synthase Activity.
Cancer Res., April 1, 2006; 66(7): 3726 - 3736.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
V. Mollace, C. Muscoli, E. Masini, S. Cuzzocrea, and D. Salvemini
Modulation of Prostaglandin Biosynthesis by Nitric Oxide and Nitric Oxide Donors
Pharmacol. Rev., June 1, 2005; 57(2): 217 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A.-M. Simeone, Y.-J. Li, L. D. Broemeling, M. M. Johnson, M. Tuna, and A. M. Tari
Cyclooxygenase-2 Is Essential for HER2/neu to Suppress N- (4-Hydroxyphenyl)retinamide Apoptotic Effects in Breast Cancer Cells
Cancer Res., February 15, 2004; 64(4): 1224 - 1228.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
H. X Nguyen and J. G Tidball
Expression of a muscle-specific, nitric oxide synthase transgene prevents muscle membrane injury and reduces muscle inflammation during modified muscle use in mice
J. Physiol., July 15, 2003; 550(2): 347 - 356.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-B. Yoon, S.-J. Kim, S.-G. Hwang, S. Chang, S.-S. Kang, and J.-S. Chun
Non-steroidal Anti-inflammatory Drugs Inhibit Nitric Oxide-induced Apoptosis and Dedifferentiation of Articular Chondrocytes Independent of Cyclooxygenase Activity
J. Biol. Chem., April 18, 2003; 278(17): 15319 - 15325.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
N. K. Chanani, D. B. Cowan, K. Takeuchi, D. N. Poutias, L. M. Garcia, P. J. del Nido, and F. X. McGowan Jr
Differential Effects of Amrinone and Milrinone Upon Myocardial Inflammatory Signaling
Circulation, September 24, 2002; 106(12_suppl_1): I-284 - I-289.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. von Knethen and B. Brune
Activation of Peroxisome Proliferator-Activated Receptor {gamma} by Nitric Oxide in Monocytes/Macrophages Down-Regulates p47phox and Attenuates the Respiratory Burst
J. Immunol., September 1, 2002; 169(5): 2619 - 2626.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
R. Bolli, K. Shinmura, X.-L. Tang, E. Kodani, Y.-T. Xuan, Y. Guo, and B. Dawn
Discovery of a new function of cyclooxygenase (COX)-2: COX-2 is a cardioprotective protein that alleviates ischemia/reperfusion injury and mediates the late phase of preconditioning
Cardiovasc Res, August 15, 2002; 55(3): 506 - 519.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. Liversidge, A. Dick, and S. Gordon
Nitric Oxide Mediates Apoptosis Through Formation of Peroxynitrite and Fas/Fas-Ligand Interactions in Experimental Autoimmune Uveitis
Am. J. Pathol., March 1, 2002; 160(3): 905 - 916.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. H. Klimp, H. Hollema, C. Kempinga, A. G. J. van der Zee, E. G. E. de Vries, and T. Daemen
Expression of Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in Human Ovarian Tumors and Tumor-associated Macrophages
Cancer Res., October 1, 2001; 61(19): 7305 - 7309.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
O. Kobayashi, H. Miwa, S. Watanabe, M. Tsujii, R. N. Dubois, and N. Sato
Cyclooxygenase-2 downregulates inducible nitric oxide synthase in rat intestinal epithelial cells
Am J Physiol Gastrointest Liver Physiol, September 1, 2001; 281(3): G688 - G696.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. MANDERSCHEID, U. K. MEßMER, R. FRANZEN, and J. PFEILSCHIFTER
Regulation of Inhibitor of Apoptosis Expression by Nitric Oxide and Cytokines: Relation to Apoptosis Induction in Rat Mesangial Cells and RAW 264.7 Macrophages
J. Am. Soc. Nephrol., June 1, 2001; 12(6): 1151 - 1163.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Perez-Sala, E. Cernuda-Morollon, M. Diaz-Cazorla, F. Rodriguez-Pascual, and S. Lamas
Posttranscriptional regulation of human iNOS by the NO/cGMP pathway
Am J Physiol Renal Physiol, March 1, 2001; 280(3): F466 - F473.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y.-W. E. Chang, R. Jakobi, A. McGinty, M. Foschi, M. J. Dunn, and A. Sorokin
Cyclooxygenase 2 Promotes Cell Survival by Stimulation of Dynein Light Chain Expression and Inhibition of Neuronal Nitric Oxide Synthase Activity
Mol. Cell. Biol., November 15, 2000; 20(22): 8571 - 8579.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
K. Notoya, D. V. Jovanovic, P. Reboul, J. Martel-Pelletier, F. Mineau, and J.-P. Pelletier
The Induction of Cell Death in Human Osteoarthritis Chondrocytes by Nitric Oxide Is Related to the Production of Prostaglandin E2 Via the Induction of Cyclooxygenase-2
J. Immunol., September 15, 2000; 165(6): 3402 - 3410.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H.-F. Cheng, J.-L. Wang, M.-Z. Zhang, James. A. McKanna, and R. C. Harris
Nitric oxide regulates renal cortical cyclooxygenase-2 expression
Am J Physiol Renal Physiol, July 1, 2000; 279(1): F122 - F129.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. M. MEI, N. G. HORD, D. F. WINTERSTEIN, S. P. DONALD, and J. M. PHANG
Expression of prostaglandin endoperoxide H synthase-2 induced by nitric oxide in conditionally immortalized murine colonic epithelial cells
FASEB J, June 1, 2000; 14(9): 1188 - 1201.
[Abstract] [Full Text]

Home page
 BloodHome page
E. Battinelli and J. Loscalzo
Nitric oxide induces apoptosis in megakaryocytic cell lines
Blood, June 1, 2000; 95(11): 3451 - 3459.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
X. Yang, N. Ma, M. J. Szabolcs, J. Zhong, E. Athan, R. R. Sciacca, R. E. Michler, G. D. Anderson, J. F. Wiese, K. M. Leahy, et al.
Upregulation of COX-2 During Cardiac Allograft Rejection
Circulation, February 1, 2000; 101(4): 430 - 438.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
K. B. Sandau, D. Callsen, and B. Brüne
Protection against Nitric Oxide-Induced Apoptosis in Rat Mesangial Cells Demands Mitogen-Activated Protein Kinases and Reduced Glutathione
Mol. Pharmacol., October 1, 1999; 56(4): 744 - 751.
[Abstract] [Full Text]

Home page
 Reproductive SciencesHome page
R. M. Moore, D. W. Lundgren, and J. J. Moore
Cyclooxygenase Inhibitors Decrease Apoptosis Initiated by Actinomycin D, Cycloheximide, and Staurosporine in Amnion-Derived WISH Cells
Reproductive Sciences, September 1, 1999; 6(5): 245 - 251.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
A. von Knethen, D. Callsen, and B. Brune
Superoxide Attenuates Macrophage Apoptosis by NF-{kappa}B and AP-1 Activation That Promotes Cyclooxygenase-2 Expression
J. Immunol., September 1, 1999; 163(5): 2858 - 2866.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Glockzin, A. von Knethen, M. Scheffner, and B. Brune
Activation of the Cell Death Program by Nitric Oxide Involves Inhibition of the Proteasome
J. Biol. Chem., July 9, 1999; 274(28): 19581 - 19586.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. DÍAZ-CAZORLA, D. PÉREZ-SALA, and S. LAMAS
Dual Effect of Nitric Oxide Donors on Cyclooxygenase-2 Expression in Human Mesangial Cells
J. Am. Soc. Nephrol., May 1, 1999; 10(5): 943 - 952.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. R. Adderley and D. J. Fitzgerald
Oxidative Damage of Cardiomyocytes Is Limited by Extracellular Regulated Kinases 1/2-mediated Induction of Cyclooxygenase-2
J. Biol. Chem., February 19, 1999; 274(8): 5038 - 5046.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. von Knethen, D. Callsen, and B. Brüne
NF-kappa B and AP-1 Activation by Nitric Oxide Attenuated Apoptotic Cell Death in RAW 264.7 Macrophages
Mol. Biol. Cell, February 1, 1999; 10(2): 361 - 372.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Ambs, M. O. Ogunfusika, W. G. Merriam, W. P. Bennett, T. R. Billiar, and C. C. Harris
Up-regulation of inducible nitric oxide synthase expression in cancer-prone p53 knockout mice
PNAS, July 21, 1998; 95(15): 8823 - 8828.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Kim, O. Bang, Y. Lee, and S. Kang
Production of inducible nitric oxide is required for monocytic differentiation of U937 cells induced by vitamin E-succinate
J. Cell Sci., January 2, 1998; 111(4): 435 - 441.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. A. Mitchell, H. Liao, J. Chesney, G. Fingerle-Rowson, J. Baugh, J. David, and R. Bucala
Macrophage migration inhibitory factor (MIF) sustains macrophage proinflammatory function by inhibiting p53: Regulatory role in the innate immune response
PNAS, January 8, 2002; 99(1): 345 - 350.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by The Federation of American Societies for Experimental Biology.