| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The FASEB Journal, Vol 1, 51-54, Copyright © 1987 by The Federation of American Societies for Experimental Biology
RESEARCH COMMUNICATIONS |
MM Cornwell, T Tsuruo, MM Gottesman and I Pastan
The photoaffinity reagent 8-azido-alpha-[32P]ATP was used to label a protein of 170 kDa in membrane vesicle preparations from a highly multidrug-resistant cell line, KB-V1, but not from the drug-sensitive parental cell line KB-3-1. The 170-kDa labeled protein was immunoprecipitated with a monoclonal antibody (MRK-16) to P glycoprotein. Both ATP and GTP inhibited labeling by 8-azido-alpha- [32P]ATP. Labeling of P170 was not inhibited by 5 mM ADP, 5 mM ribose-5- phosphate, or 100 microM vinblastine. These data directly demonstrate that P glycoprotein has a nucleotide-binding site that could supply energy for drug transport.
This article has been cited by other articles:
|
|
 |
|
  D.-W. Shen, X.-J. Liang, M. A. Gawinowicz, and M. M. Gottesman Identification of Cytoskeletal [14C]Carboplatin-Binding Proteins Reveals Reduced Expression and Disorganization of Actin and Filamin in Cisplatin-Resistant Cell Lines Mol. Pharmacol., October 1, 2004; 66(4): 789 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Yang, A. D. Vassil, and W. N. Hait Activation of Phospholipase C Induces the Expression of the Multidrug Resistance (MDR1) Gene through the Raf-MAPK Pathway Mol. Pharmacol., October 1, 2001; 60(4): 674 - 680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gao, H.-R. Cui, D. W. Loe, C. E. Grant, K. C. Almquist, S. P. C. Cole, and R. G. Deeley Comparison of the Functional Characteristics of the Nucleotide Binding Domains of Multidrug Resistance Protein 1 J. Biol. Chem., April 21, 2000; 275(17): 13098 - 13108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Szabo, E. Welker, E. Bakos, M. Muller, I. Roninson, A. Varadi, and B. Sarkadi Drug-stimulated Nucleotide Trapping in the Human Multidrug Transporter MDR1. COOPERATION OF THE NUCLEOTIDE BINDING DOMAINS J. Biol. Chem., April 24, 1998; 273(17): 10132 - 10138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Marbeuf-Gueye, H. J. Broxterman, F. Dubru, W. Priebe, and A. Garnier-Suillerot Kinetics of Anthracycline Efflux from Multidrug Resistance Protein-Expressing Cancer Cells Compared with P-Glycoprotein-Expressing Cancer Cells Mol. Pharmacol., January 1, 1998; 53(1): 141 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kast, V. Canfield, R. Levenson, and P. Gros Transmembrane Organization of Mouse P-glycoprotein Determined by Epitope Insertion and Immunofluorescence J. Biol. Chem., April 19, 1996; 271(16): 9240 - 9248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Borgnia, G. D. Eytan, and Y. G. Assaraf Competition of Hydrophobic Peptides, Cytotoxic Drugs, and Chemosensitizers on a Common P-glycoprotein Pharmacophore as Revealed by Its ATPase Activity J. Biol. Chem., February 9, 1996; 271(6): 3163 - 3171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Müller, Év. Bakos, E. Welker, A.ás Váradi, U. A. Germann, M. M. Gottesman, B. S. Morse, I. B. Roninson, and B.áz. Sarkadi Altered Drug-stimulated ATPase Activity in Mutants of the Human Multidrug Resistance Protein J. Biol. Chem., January 26, 1996; 271(4): 1877 - 1883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Beaudet and P. Gros Functional Dissection of P-glycoprotein Nucleotide-binding Domains in Chimeric and Mutant Proteins J. Biol. Chem., July 21, 1995; 270(29): 17159 - 17170. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
