FASEB J. Mp Biomedicals
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
17/8/881
02-0615fjev1    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Linn, T.
Right arrow Articles by Bretzel, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Linn, T.
Right arrow Articles by Bretzel, R. G.
The FASEB Journal Express Article doi:10.1096/fj.02-0615fje
Published online March 28, 2003

Angiogenic capacity of endothelial cells in islets of Langerhans

T. Linn, K. Schneider, H. P. Hammes, K. T. Preissner, H. Brandhorst, E. Morgenstern, F. Kiefer, and R. G. Bretzel

E-mail contact: thomas.linn{at}innere.med.uni-giessen.de

Transplantation of pancreatic islets reconstitutes glucose homeostasis in diabetes mellitus. Before transplantation, islets are disrupted from the surrounding blood vessels by the isolation procedure, with the grafted tissue being subject to ischemic damage. The survival of transplanted islets is assumed to depend on effective revascularization. Perfusion studies suggest that newly formed microvessels supplying the graft with nutrients are exclusively rebuilt by the host. It is generally not known whether isolated islets contain endothelial cells (EC), which potentially participate in the revascularization process. Therefore, we tried to detect immature EC in isolated islets by transformation with polyoma middle T antigen. Endothelioma cells were generated, implicating the presence of de-differentiated EC within isolated islets. When embedded in a fibrin gel, the islets developed cellular cords consisting of EC, whereas FGF-2 and VEGF stimulated the formation of cord-like structures. Furthermore, we studied the presence of donor EC in islet grafts by using transgenic mice with an EC lineage-specific promotor-LacZ reporter construct (Tie-2LacZ). Following islet transplantation, Tie-2LacZ-positive EC of both donor and recipient were identified in the vicinity of or within the graft up to 3 wk after transplantation. In conclusion, EC and/or their progenitors with angiogenic capacity reside within isolated islets of different species, and their proliferative potential can be stimulated by various inducers. These graft-related endothelia persist after islet transplantation and are integrated within newly formed microvessels.

Key words: islet transplantation · vascularization · Tie-2 LacZ




This article has been cited by other articles:


Home page
 EndocrinologyHome page
M. Johansson, J. Olerud, L. Jansson, and P.-O. Carlsson
Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets
Endocrinology, April 1, 2009; 150(4): 1646 - 1653.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J. Olerud, M. Johansson, J. Lawler, N. Welsh, and P.-O. Carlsson
Improved Vascular Engraftment and Graft Function After Inhibition of the Angiostatic Factor Thrombospondin-1 in Mouse Pancreatic Islets
Diabetes, July 1, 2008; 57(7): 1870 - 1877.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
D. Su, N. Zhang, J. He, S. Qu, S. Slusher, R. Bottino, S. Bertera, J. Bromberg, and H. H. Dong
Angiopoietin-1 Production in Islets Improves Islet Engraftment and Protects Islets From Cytokine-Induced Apoptosis
Diabetes, September 1, 2007; 56(9): 2274 - 2283.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. Brissova, A. Shostak, M. Shiota, P. O. Wiebe, G. Poffenberger, J. Kantz, Z. Chen, C. Carr, W. G. Jerome, J. Chen, et al.
Pancreatic Islet Production of Vascular Endothelial Growth Factor-A Is Essential for Islet Vascularization, Revascularization, and Function
Diabetes, November 1, 2006; 55(11): 2974 - 2985.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
N. Zhang, D. Su, S. Qu, T. Tse, R. Bottino, A.N. Balamurugan, J. Xu, J. S. Bromberg, and H. H. Dong
Sirolimus Is Associated With Reduced Islet Engraftment and Impaired {beta}-Cell Function
Diabetes, September 1, 2006; 55(9): 2429 - 2436.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
D. Nyqvist, M. Kohler, H. Wahlstedt, and P.-O. Berggren
Donor Islet Endothelial Cells Participate in Formation of Functional Vessels Within Pancreatic Islet Grafts
Diabetes, August 1, 2005; 54(8): 2287 - 2293.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. Bottino, A.N. Balamurugan, H. Tse, C. Thirunavukkarasu, X. Ge, J. Profozich, M. Milton, A. Ziegenfuss, M. Trucco, and J. D. Piganelli
Response of Human Islets to Isolation Stress and the Effect of Antioxidant Treatment
Diabetes, October 1, 2004; 53(10): 2559 - 2568.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. M. Harlan
Gene-Altered Islets for Transplant: Giant Leap or Small Step?
Endocrinology, February 1, 2004; 145(2): 463 - 466.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2003 by The Federation of American Societies for Experimental Biology.