Figure 2. Regulation of apelin expression by TNF±. in 3T3F442A adipocytes. A) Dose response effect of 8 h TNF ± treatment on apelin mRNA levels in 10-day-differentiated 3T3F442A adipocytes. Results are mean values ± SEM from 3 separate experiments. B) Apelin mRNA levels in 10-day-differentiated 3T3F442A adipocytes after 24 h serum deprivation and incubated 8 h or not with 10 ng/ml TNF± in the absence (–) or presence (+; added 1 h before TNF± treatment) of LY294002 (10 µM), PD098059 (50 µM), GF109203X (10 µM), or SP 600125, (20 µM). Results are mean values ± SEM from 4 separate experiments, *P < 0.05 when compared with controls; P < 0.05 when compared with TNF±-stimulated cells.