Figure 1. Hydrogen peroxide-induced apoptosis is exacerbated following cholesterol depletion. Bovine aortic endothelial cells (BAEC) were pretreated with 10 mM methyl-ß-cyclodextrin (CD) for 30 min followed by bolus administration of H₂O₂ (500 µM). Representative fluorescence micrographs showing H₂O₂ induced apoptosis, as detected by TUNEL assay, after 4 h of exposure. Data are reported as mean ± SD; *P < 0.05. Comparisons between H₂O₂-treated cells with and without CD were significant at P < 0.05 (#).